LOCUS       sequence-343203-       10041 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression vector with Shield-1 stabilizable hSOX17 fused to 
            TagBFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Wu HJ, Landshammer A, Stamenova EK, Bolondi A, Kretzmer H, Meissner 
            A, Michor F
  TITLE     Topological isolation of developmental regulators in mammalian 
            genomes.
  JOURNAL   Nat Commun. 2021 Aug 12;12(1):4897. doi: 10.1038/s41467-021-24951-7.
  PUBMED    34385432
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-08-12"; volume: "12(1):4897. doi"; pages: " 
            10.1038/s41467-021-24951-7"
COMMENT     Sequence Label: PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA
FEATURES             Location/Qualifiers
     source          1..10041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          658..1673
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1597..1619
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1681..1700
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1766..1775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1772..1774,1775..2095)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             2096..2161
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2168..2188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3158..3175
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3164..3181
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3497..4192
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             4193..4240
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4295..4312)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4301..4525
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4610..4727
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4773..4806
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   4977..5039
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(5063..5081)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5102..5118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5102..5118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5115..5137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5126..5142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5150..5156,5157..5174,5175..5180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5195..5216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5333..5350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5504..6092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5584..5603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6263..7054,7055..7123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6886..6905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7124..7228)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7254..7709)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7391..7412)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7603..7622
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7836..7858
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7850..7867
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7851..7867
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7874..7893
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7874..7892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7911..7945
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(8427..9452)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8427..9452)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9471..9970)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9519..9538)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9912..9935
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    9993..10026
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgccr gggggggggg gggggggggg gggggggggg
      541 ggggsgrggs ggrgrggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     1141 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     1201 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     1261 cgcctcgggc cggggrgggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcctc gacggtatcg
     1741 ataagctatc acaagtttgt acattgccac catgggagtg caggtggaaa ccatctcccc
     1801 aggtgacggg cgcaccttcc ccaagcgcgg ccagacctgt gtggtgcact acaccgggat
     1861 gcttgaagat ggaaagaaag tcgattcctc ccgggacaga aacaagccct ttaagtttat
     1921 gctaggcaag caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg
     1981 tcagagagcc aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg
     2041 catcatccca ccacatgcca ctctcgtctt cgatgtggag cttctaaaac cggaagacta
     2101 taaggaccac gacggagact acaaggatca tgatattgat tacaaagacg atgacgataa
     2161 gatggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccagcagccc
     2221 ggatgcggga tacgccagtg acgaccagag ccagacccag agcgcgctgc ccgcggtgat
     2281 ggccgggctg ggcccctgcc cctgggccga gtcgctgagc cccatcgggg acatgaaggt
     2341 gaagggcgag gcgccggcga acagcggagc accggccggg gccgcgggcc gagccaaggg
     2401 cgagtcccgt atccggcggc cgatgaacgc tttcatggtg tgggctaagg acgagcgcaa
     2461 gcggctggcg cagcagaatc cagacctgca caacgccgag ttgagcaaga tgctgggcaa
     2521 gtcgtggaag gcgctgacgc tggcggagaa gcggcccttc gtggaggagg cagagcggct
     2581 gcgcgtgcag cacatgcagg accaccccaa ctacaagtac cggccgcggc ggcgcaagca
     2641 ggtgaagcgg ctgaagcggg tggagggcgg cttcctgcac ggcctggctg agccgcaggc
     2701 ggccgcgctg ggccccgagg gcggccgcgt ggccatggac ggcctgggcc tccagttccc
     2761 cgagcagggc ttccccgccg gcccgccgct gctgcctccg cacatgggcg gccactaccg
     2821 cgactgccag agtctgggcg cgcctccgct cgacggctac ccgttgccca cgcccgacac
     2881 gtccccgctg gacggcgtgg accccgaccc ggctttcttc gccgccccga tgcccgggga
     2941 ctgcccggcg gccggcacct acagctacgc gcaggtctcg gactacgctg gccccccgga
     3001 gcctcccgcc ggtcccatgc acccccgact cggcccagag cccgcgggtc cctcgattcc
     3061 gggcctcctg gcgccaccca gcgcccttca cgtgtactac ggcgcgatgg gctcgcccgg
     3121 ggcgggcggc gggcgcggct tccagatgca gccgcaacac cagcaccagc accagcacca
     3181 gcaccacccc ccgggccccg gacagccgtc gccccctccg gaggcactgc cctgccggga
     3241 cggcacggac cccagtcagc ccgccgagct cctcggggag gtggaccgca cggaatttga
     3301 acagtatctg cacttcgtgt gcaagcctga gatgggcctc ccctaccagg ggcatgactc
     3361 cggtgtgaat ctccccgaca gccacggggc catttcctcg gtggtgtccg acgccagctc
     3421 cgcggtatat tactgcaact atcctgacgt gggtggcggc ggatctggtg gcggtggctc
     3481 gggcggtggt gggtcgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     3541 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     3601 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     3661 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     3721 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     3781 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     3841 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     3901 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     3961 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac
     4021 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     4081 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     4141 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta ataaaaggcc
     4201 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag taataataag atccacgcgt
     4261 ctcgagttaa cggtacctct agagaattcg ctagcctcga ctgtgccttc tagttgccag
     4321 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4381 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4441 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4501 gctggggatg cggtgggctc tatggcttct gaggcggtta attaaaataa aactagagcg
     4561 ggcccggtac taaccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
     4621 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     4681 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
     4741 ctgccatttg tctgcagagc ggccgcgtcg agataacttc gtataatgta tgctatacga
     4801 agttatgttt tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa
     4861 ataaacataa ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa
     4921 aacttaatat ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg
     4981 cgtcaatttt acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt
     5041 taaagagctc cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
     5101 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5161 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5221 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5281 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5341 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5401 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5461 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5521 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5581 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5641 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5701 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5761 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5821 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5881 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5941 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6001 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6061 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6121 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6181 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6241 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6301 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6361 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6421 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6481 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6541 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6601 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6661 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6721 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6781 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6841 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6901 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6961 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7021 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     7261 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     7321 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     7381 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     7441 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     7501 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     7561 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     7621 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     7681 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     7741 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     7801 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     7861 ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccttaa ccctagaaag
     7921 atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc
     7981 gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg
     8041 tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat
     8101 gacgcatgat tatcttttac gtgactttta agatttaact catacgataa ttatattgtt
     8161 atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt atagatatcc
     8221 aactatgaaa cattatcata aagcaatagt aaagagcctt cagtaaagag caggcattta
     8281 tctaatccca ccccaccccc acccccgtag ctccaatcct tccattcaaa atgtaggtac
     8341 tctgttctca cccttcttaa caaagtatga caggaaaaac ttccatgtgg acatctttat
     8401 tgtttaatag atcatcaatt tctcgactat tcctttgccc tcggacgagt gctggggcgt
     8461 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     8521 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     8581 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     8641 agaccaatgc ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc
     8701 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     8761 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     8821 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     8881 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     8941 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     9001 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     9061 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
     9121 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     9181 atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc
     9241 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     9301 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     9361 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
     9421 acagacgtcg cggtgagttc aggctttttc atggtggcgg ctggatcggt cgaaaggccc
     9481 ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc
     9541 gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc
     9601 ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag ctgctattgg
     9661 ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga
     9721 ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg
     9781 gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga
     9841 gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag
     9901 cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc
     9961 tcccctaccc ggtagaattc atcgatgata tcataacttc gtataatgta tgctatacga
    10021 agttatgcgg ccctcgaggt c
//
