LOCUS       sequence-343245-        6575 bp    DNA     circular SYN 14-APR-2025
DEFINITION  AAV transfer plasmid for neuronal expression of soma-targeted 
            (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence 
            attaching the ribosomal tag to the jGCaMP8s protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: AAV-hSyn-RiboL1-jGCaMP8s
FEATURES             Location/Qualifiers
     source          1..6575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        183..630
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             667..684
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     966..987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1093..1114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1354..1373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2702..3290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2755..2775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3173..3184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3322..3798
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3521..3540)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3838..3978
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3838..3967
                     /label=AAV2 ITR
     rep_origin      4053..4508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4140..4159)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4350..4371
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4525..4544)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4644..4666
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4704..4722)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4790..4894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4895..4963,4964..5755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5113..5132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5926..6514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6415..6434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaatt aagtgtctag actgcagagg gccctgcgta
      181 tgagtgcaag tgggttttag gaccaggatg aggcggggtg ggggtgccta cctgacgacc
      241 gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca
      301 gagaggggga ggggaaacag gatgcggcga ggctcgtgcg cactgccagc ttcagcaccg
      361 cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc
      421 gcgctgacgt cactcgccgg tcccccgcaa actccccttc ccggccacct tggtcgcgtc
      481 cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg
      541 cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc
      601 ggaggagtcg tgtcgtgcct gagagcgcag tcgagaaacc ggctagagga tccacccgcc
      661 accatgcatc atcaccatca tcacacgcgt cgcaagaaga ccttcaagga ggtggccacc
      721 gccgtgaaga tcatcgccat gctgatgggc ctgaagatca acgtctatat caaggccgac
      781 aagcagaaga acggcatcaa ggcgaacttc cacatccgcc acaacatcga ggacggcggc
      841 gtgcagctcg cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      901 cccgacaacc actacctgag cgtggagtcc aaactttcga aagaccccaa cgagaagcgc
      961 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1021 ctgtacaagg gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg
     1081 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1141 ggcgagggtg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1201 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1261 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1321 ggctacatcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1381 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1441 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acctgccgga ccaactgact
     1501 gaagagcaga tcgcagaata caaagaggct ttctccctat ttgacaagga cggggatggg
     1561 acaataacaa ccaaggagct ggggacggtg atgcggtctc tcggacacaa ccccacagaa
     1621 gcagagctgc aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc
     1681 cctgagttcc tgacaatgat ggcaagaaaa atgaaataca gggacacgga agaagaaatt
     1741 agagaagcgt tcggtgtgtt tgataaggat ggcaatggct acatcagtgc agcagagctt
     1801 cgccacgtga tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc
     1861 agggaagcag acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg
     1921 acagcgaagg ggggcagcgg tgggtctgga gggactgggg gtagcggtgg tagcggtggg
     1981 acagggggaa gcggcggtag tggtggaaca agcagcaaag tctcacgcga caccctgtac
     2041 gaggcggtgc gggaagtcct gcacgggaac cagcgcaagc gccgcaagtt tctggagacg
     2101 gtggagctgc agatcagcct gaagaactac gaccctcaga aggacaaacg tttctcgggc
     2161 accgtcaggc tcaagtccac cccacgcccc aagttctcgg tgtgcgttct gggggaccag
     2221 cagcactgtg atgaagccaa ggccgtggat atcccccaca tggacatcga ggcgctcaag
     2281 aaacttaaca aaaacaagaa gttggtcaag aagctggcta agaagtacga tgcctttttg
     2341 gcctctgagt ctctgattaa gcagatccca cgtatcctgg gcccaggcct aaacaaggct
     2401 ggcaagttcc cctccctgct gacacacaat gaaaacatgg tggccaaagt ggatgaggtg
     2461 aaatcgacaa tcaagttcca gatgaagaag gtgctgtgtt tggccgtcgc tgttggccac
     2521 gtgaagatga ccgatgatga gctagtctac aacattcatc tggctgtcaa tttcttggtg
     2581 tccttgctta agaaaaactg gcaaaacgtg cgggctctgt acatcaagag caccatgggc
     2641 aagccccagc gtctgtatta gggcgcgccc ctgcagggaa ttcgatatca agcttatcga
     2701 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2761 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2821 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2881 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2941 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3001 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3061 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3121 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3181 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3241 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3301 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3361 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3421 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3481 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3541 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3601 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3661 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     3721 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     3781 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     3841 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3901 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3961 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4021 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     4081 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     4141 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4201 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4261 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4321 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4381 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4441 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4501 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4561 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4621 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4681 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4741 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4801 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4861 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4921 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4981 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5041 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5101 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5161 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5221 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5281 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5341 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5401 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5461 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5521 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5581 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5641 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5701 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5761 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5821 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5881 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5941 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6001 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6061 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6541 gttcctggcc ttttgctggc cttttgctca catgt
//
