LOCUS       sequence-343324-       10218 bp    DNA     circular SYN 15-APR-2025
DEFINITION  lentiviral vector for VAMP4 knock-down in rat cells, KD1 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10218)
  TITLE     The vSNAREs VAMP2 and VAMP4 control recycling and intracellular 
            sorting of post-synaptic receptors in neuronal dendrites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: VAMP4 KD1 mScarlet
FEATURES             Location/Qualifiers
     source          1..10218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..77)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        630..828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             846..1026
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1073..1198
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1691..1924
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2109..2153
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2451..2568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2621..2835
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2756..2775
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        2939..4150
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3987..4006
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4179..4188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4185..4880
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(4318..4336)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4586..4605
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4927..5515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4980..5000)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5398..5409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6034..6214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6240..6257)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6246..6470
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6516..6944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6603..6622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6813..6834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6953..6973)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6958..7287
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7138..7273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7200..7219
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7335..7382
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7401..7775
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7905..8026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7942..7961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7996..8015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8075..8091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8075..8091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8088..8110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8099..8115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8123..8129,8130..8147,8148..8153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8168..8189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8306..8323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8477..9065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8557..8576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9236..10027,10028..10096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9859..9878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10097..10201)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
       61 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
      121 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
      181 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
      241 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
      841 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      901 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      961 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1021 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     1081 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     1141 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     1201 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     1261 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     1321 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1381 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     1441 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     1501 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     1561 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1621 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1681 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1741 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1801 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1861 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1921 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1981 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     2041 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     2101 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     2161 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     2221 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     2281 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     2341 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     2401 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     2461 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2521 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2581 agggacagca gagatccagt ttggttaatc gtacgaattc gaacgctgac gtcatcaacc
     2641 cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc
     2701 tggcaggaag atggctgtga gggacagggg agtggcgccc tgcaatattt gcatgtcgct
     2761 atgtgttctg ggaaatcacc ataaacgtga aatgtctttg gatttgggaa tcttataagt
     2821 tctgtatgag accacagatc cccctatctt tatttaacaa cattcaagag atgttgttaa
     2881 ataaagatag tttttctcga gcttatcgaa ttaattaacc cgtgtcggct ccagatctgg
     2941 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     3001 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     3061 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     3121 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     3181 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     3241 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     3301 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     3361 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     3421 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     3481 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     3541 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     3601 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     3661 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     3721 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     3781 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     3841 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     3901 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     3961 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     4021 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     4081 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     4141 tttgttagac gaagcttggg ctgcaggtcg actctagagc caccatggtg agcaagggcg
     4201 aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc
     4261 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     4321 agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt
     4381 tcatgtacgg ctccagggcc ttcatcaagc accccgccga catccccgac tactataagc
     4441 agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg
     4501 tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc
     4561 gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag
     4621 cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt aagatggccc
     4681 tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa gaccacctac aaggccaaga
     4741 agcccgtgca gatgcccggc gcctacaacg tcgatcgcaa gttggacatc acctcccaca
     4801 acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac tccaccggcg
     4861 gcatggacga gctgtacaag taaaccggtg tcgagctaga attaattcga tatcaagctt
     4921 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4981 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5041 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5101 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5161 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5221 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5281 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5341 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5401 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5461 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     5521 taccgtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     5581 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     5641 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5701 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     5761 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     5821 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     5881 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     5941 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     6001 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     6061 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6121 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6181 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     6241 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6301 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6361 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6421 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6481 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6541 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6601 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6661 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6721 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6781 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6841 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6901 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     6961 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7021 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7081 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7141 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7201 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7261 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7321 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7381 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     7441 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     7501 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     7561 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     7621 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     7681 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     7741 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     7801 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7861 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7921 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7981 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8041 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8101 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8161 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8281 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8881 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9541 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9841 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10081 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10141 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10201 gcacatttcc ccgaaaag
//
