LOCUS       sequence-343343-       10218 bp    DNA     circular SYN 15-APR-2025
DEFINITION  lentiviral vector for VAMP4 knock-down in rat cells, KD2 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10218)
  TITLE     The vSNAREs VAMP2 and VAMP4 control recycling and intracellular 
            sorting of post-synaptic receptors in neuronal dendrites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: VAMP4 KD2 mScarlet
FEATURES             Location/Qualifiers
     source          1..10218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     264..283
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      456..465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             462..1157
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(595..613)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     863..882
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1204..1792
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1257..1277)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1675..1686)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2311..2491
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2517..2534)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2523..2747
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2793..3221
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2880..2899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3090..3111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3230..3250)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3235..3564
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3415..3550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3477..3496
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3612..3659
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3678..4052
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4182..4303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4219..4238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4273..4292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4352..4368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4352..4368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4365..4387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4376..4392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4400..4406,4407..4424,4425..4430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4445..4466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4583..4600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4754..5342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4834..4853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5513..6304,6305..6373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6136..6155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6374..6478)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6553..6572)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6744..7123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7125..7323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7278..7298
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7341..7521
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7568..7693
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8186..8419
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8604..8648
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8946..9063
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9116..9330
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     9251..9270
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        join(9434..10218,1..427)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
       61 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
      121 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
      181 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
      241 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
      301 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
      361 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
      421 gttagacgaa gcttgggctg caggtcgact ctagagccac catggtgagc aagggcgagg
      481 cagtgatcaa ggagttcatg cggttcaagg tgcacatgga gggctccatg aacggccacg
      541 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      601 tgaaggtgac caagggtggc cccctgccct tctcctggga catcctgtcc cctcagttca
      661 tgtacggctc cagggccttc atcaagcacc ccgccgacat ccccgactac tataagcagt
      721 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgccgtga
      781 ccgtgaccca ggacacctcc ctggaggacg gcaccctgat ctacaaggtg aagctccgcg
      841 gcaccaactt ccctcctgac ggccccgtaa tgcagaagaa gacaatgggc tgggaagcgt
      901 ccaccgagcg gttgtacccc gaggacggcg tgctgaaggg cgacattaag atggccctgc
      961 gcctgaagga cggcggccgc tacctggcgg acttcaagac cacctacaag gccaagaagc
     1021 ccgtgcagat gcccggcgcc tacaacgtcg atcgcaagtt ggacatcacc tcccacaacg
     1081 aggactacac cgtggtggaa cagtacgaac gctccgaggg ccgccactcc accggcggca
     1141 tggacgagct gtacaagtaa accggtgtcg agctagaatt aattcgatat caagcttatc
     1201 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1261 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1321 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     1381 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     1441 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     1501 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     1561 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     1621 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     1681 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     1741 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     1801 cgtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     1861 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     1921 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     1981 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     2041 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     2101 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     2161 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     2221 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     2281 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     2341 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     2401 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     2461 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     2521 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2581 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     2641 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     2701 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     2761 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     2821 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2881 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2941 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3001 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3061 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3121 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3181 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     3241 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3301 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3361 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3421 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3481 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     3541 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     3601 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     3661 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     3721 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     3781 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     3841 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     3901 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     3961 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     4021 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     4081 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4141 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     4201 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4261 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     4321 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4381 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4441 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4501 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4561 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4621 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4681 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4741 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4801 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4861 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4921 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4981 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5041 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5101 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5161 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5221 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5281 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5341 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5401 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5461 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5521 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5581 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5641 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5701 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5761 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5821 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5881 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5941 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6001 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6061 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6121 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6181 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6241 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6301 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6361 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6421 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6481 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     6541 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     6601 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     6661 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     6721 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     6781 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     6841 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     6901 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     6961 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     7021 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     7081 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     7141 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     7201 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     7261 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     7321 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7381 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7441 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7501 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     7561 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     7621 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     7681 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     7741 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     7801 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     7861 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     7921 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7981 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8041 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8101 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8161 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8221 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     8281 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     8341 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     8401 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     8461 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     8521 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     8581 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     8641 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     8701 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     8761 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     8821 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     8881 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     8941 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     9001 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     9061 tttcgggttt attacaggga cagcagagat ccagtttggt taatcgtacg aattcgaacg
     9121 ctgacgtcat caacccgctc caaggaatcg cgggcccagt gtcactaggc gggaacaccc
     9181 agcgcgcgtg cgccctggca ggaagatggc tgtgagggac aggggagtgg cgccctgcaa
     9241 tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt
     9301 gggaatctta taagttctgt atgagaccac agatccccgg accatctgga ccaagatttc
     9361 aagagaatct tggtccagat ggtccttttt ctcgagctta tcgaattaat taacccgtgt
     9421 cggctccaga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc
     9481 acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg
     9541 acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag
     9601 aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc
     9661 ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc
     9721 ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag
     9781 ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc
     9841 gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt
     9901 gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg
     9961 gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg
    10021 aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag
    10081 gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc
    10141 atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg
    10201 cgggggcggc agttatgg
//
