LOCUS       sequence-343611-       15339 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15339)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 15339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-jGCaMP8m
FEATURES             Location/Qualifiers
     source          1..15339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3328..3345
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     3627..3648
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3754..3775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4015..4034)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             6768..6779
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8571..8582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11778..11796)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11792..11839
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11867..11883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11867..11883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11880..11902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11891..11907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11915..11921,11922..11939,11940..11945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11960..11981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12098..12115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12269..12857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12349..12368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13028..13819,13820..13888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13651..13670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13889..13993)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14020..14476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14107..14126)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14317..14338
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14602..14624
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14616..14633
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14617..14633
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        14703..15006
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15007..15210
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     15161..15181
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     15255..15274
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15255..15273
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatgcat catcaccatc atcacacgcg tcgcaagaag
     3361 accttcaagg aggtggccac cgccgtgaag atcatcggcc gcctgatggg cctgaagatc
     3421 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt ccacatccgc
     3481 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
     3541 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtggagtc caaactttcg
     3601 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3661 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
     3721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3781 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
     3841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3901 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3961 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
     4021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4141 aacctgccgg accaactgac tgaagagcag atcgcagaat acaaagaggc tttctcccta
     4201 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     4261 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     4321 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac
     4381 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     4441 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     4501 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     4561 gaagagtttg tacaaatgat gacagcgaag taagctagcg gcgcctagcc ggtcatcctt
     4621 ttgacgattc cagtcccgag gataacctcc tctcgggatt ggggggaatc tttggatcca
     4681 gtagtcctcc ttgaactccg tccaacaggg cagattcaag agtcataaga ctttcattaa
     4741 tcatttcagt tgatcagaca tggtcgtgta gattctctta atacgggaga tcttctagca
     4801 gtttcagtga ccaacggtgc tttcattctc caggaactga taccaaaggt tgtggacagg
     4861 ccaaggggta cttcggatga ctctgtgctt gggcacagaa agaggtcgta gtgtgccccc
     4921 cgatagcgga ctcgacatga atcaactaag aaaggcaatc tgcctcccat gatggacata
     4981 agcaatagtt cacaaccatc ttgcatctca gtgaagtgta cataactatt gagggctggg
     5041 tcatctaagc atttcagtcg agaaaaaaac tgtagaccaa aagaacaact agcaacactt
     5101 ctcatccaga gacccatatc aagatgctag atccgggaga ggtttatgat gaccctattg
     5161 atccaattga gtcagaggct gaacccagag gaacccccac tgtccccaac atcttgagga
     5221 actccgacta caatctcaat tctcctttga tagaggatcc tgccaaacta atgttagaat
     5281 ggttgaagac agggaacaga ccttatcgga tgactttgac agacaattgc tccaggtctt
     5341 acaaagtttt gaaagattat ttcaagaaag tagatttggg ttctctcaaa gtgggcggaa
     5401 ctgctgcaca gtcaatggtt tctctctggt tgtgtggtgc ccactctgaa tcaaacagga
     5461 gccggagatg tataaccgac ttggcccatt tctattccaa gtcatccccc atagagaagc
     5521 tattgaattg tacgctagga aacagaggcc tgagaatccc accagagggg gtgttaaatt
     5581 gcctcgagag ggtcaattat gacaaggcat ttgggaggta tctggccaac acgtattcct
     5641 cttacttgtt tttccatgta atcaccttat acatgaatgc cttagactgg gaagaggaaa
     5701 agaccatcct ggcattatgg aaagatataa cctcagtgga taccgagaag gacttggtca
     5761 aattcaaaga tcaaatatgg ggactgttga ttgtgacaaa ggactttgtt tactctcaga
     5821 gttcaaactg tctttttgac agaaactaca cactgatgct aaaggatctt ttcttgtctc
     5881 gattcaactc cttaatgatt ctgctttctc cccctgagcc ccgatactca gatgacttaa
     5941 tatctcagct gtgccagcta tacatcgctg gggatcaagt cttgtccatg tgtgggaact
     6001 ccggctatga agtcatcaaa atattggagc catatgtcgt gaacagtttg gtccagaggg
     6061 cagagaagtt taggcctctc atccacccct tgggagactt tcctatgttc ataaaagaca
     6121 aggtgaatca acttgaaggg acttttggtc ccagtgcaaa gaggtttttt agggttttag
     6181 atcaattcga caacatacat gacttagtat ttgtgtatgg ctgttacaga cattgggggc
     6241 acccctatat agattatcgg aagggtctgt cgaaactata tgatcaagtt cacattaaga
     6301 aagtaataga taagtcctac caggagtgtt tagcaagtga cttggccaga aggatcctca
     6361 gatggggatt tgacaagtac tccaaatggt atctagattc gagattcctt gcccgagacc
     6421 accccttgac tccttatgtc aagacccaaa catggccacc caaacatata gtagacttgg
     6481 tgggggacac atggcataag ctcccgatca cgcagatctt tgaaattcct gaatcaatgg
     6541 acccgtcaga gatactggat gacaaatcac attctttcac cagaacaagg ttagcttctt
     6601 ggctgtcaga gaaccgaggg gggcctgttc ctagcgagaa ggtcattatc acggccctgt
     6661 ctaagccacc tgtcaatccc cgagagtttt tgaaatctat agacctcgga ggattgccag
     6721 atgaggattt gataattggc ctcaaaccaa aggaacggga gttgaagatc gagggccgat
     6781 tctttgctct aatgtcatgg aatctaagat tatattttgt catcaccgaa aagctcttgg
     6841 ccaactacat tttgccactt tttgacgcac tgactatgac agacaacctg aacaaggtgt
     6901 tcaaaaagtt gatcgacagg gtcaccgggc aagggctttt ggactattct agggtcacat
     6961 acgcatttca cctggactat gagaaatgga acaatcatca aagattggag tcaacagagg
     7021 atgtattctc tgtcctagat caggtgtttg gattgaagag ggtgttttct agaacacacg
     7081 agttttttca gaagtcctgg atctattatt cagacagatc agacctcatt gggttatggg
     7141 aggaccaaat atattgcttg gatatgtcta acggcccaac ctgctggaat ggccaagatg
     7201 gcgggctaga gggcttgcgg cagaagggct ggagtctagt cagtttatta atgatagata
     7261 gagaatctca aaccaggaac acaagaacca agatactagc tcaaggagac aaccaggttc
     7321 tgtgtcctac atacatgttg tcaccgggat tgtctcaaga ggggcttctc tatgagttag
     7381 agagcatatc aaggaatgca ctctcaatat accgagctat cgaggaagga gcatctaagc
     7441 tggggctgat catcaagaag gaagagacca tgtgtagtta tgactttctc atatatggga
     7501 agaccccctt atttcgaggc aacatattgg tgcctgaatc caaaagatgg gcccgagtct
     7561 cttgcatctc taacgaccaa atagtcaacc tcgccaatat aatgtcgaca gtatccacca
     7621 atgcgctgac agtggcacaa cactctcaat ctctgatcaa acctatgagg gattttctgc
     7681 tcatgtcagt acaggcagtt ttccactacc tgttgtttag cccaatccta aaaggcagag
     7741 tttataagat tctgagtgct gaaggggaga gctttctcct agccatgtcg cggataatct
     7801 acctagatcc ttctttggga ggggtgtctg gaatgtctct cgggaggttc catatacgtc
     7861 agttctcaga ccctgtctct gaagggttgt cattctggag agagatctgg ttaggctctc
     7921 atgagtcctg gattcacgcg ttgtgtcagg aggccgggaa ccccgatctt ggagagagaa
     7981 cactcgagag cttcactcgc cttttagaag atcctactac cttaaatatc aaaggagggg
     8041 ccagccctac cattctactc aaggatgcta tcagaaaggc tctgtacgac gaggtggaca
     8101 aggtggaaaa ttcagagttt cgagaggcaa tcctgctgtc caagacccat agagataact
     8161 ttatactctt tttaaaatct gttgagcctc tgttccctcg atttctcagt gagctcttca
     8221 gttcgtcttt cttgggaata ccggagtcaa tcattggact gatacaaaac tcccggacaa
     8281 taagaaggca gtttagaaag agtctctcaa gaactttaga agagtccttc tacaactcag
     8341 agatccacgg gattaatcgg ataacccaga cacctcaaag ggtcggaagg gtgtggcctt
     8401 gctcttcaga gagggcagat ctacttaggg agatctcttg ggggaggaaa gtggtaggca
     8461 cgacagttcc tcacccttcc gagatgttgg agttgtttcc caaatcctcc atttcctgca
     8521 cttgtggagc aacaggggga ggcaatccta gagtctctgt atcagtactc ccgtccttcg
     8581 atcagtcatt tttctcacgg ggccccctaa agggatactt gggctcgtcc acctccatgt
     8641 caacccagct attccatgca tgggaaaaag tcactaatgt tcatgtggtg aaaagggctc
     8701 tatcgttaaa agaatctata aactggttca tcaataggaa ttccaatttg gctcaaactc
     8761 taattggaaa catcatgtct ctgacaggcc ctgatttccc tctagaagag gcccctgttt
     8821 tcaaacggac agggtcagcc ttgcataggt tcaagtctgc cagatacagc gaaggagggt
     8881 attcttctgt ttgccctaac cttctctccc atatctctgt tagtacagac actatgtctg
     8941 atttgaccca aaacgggaag aactatgatt tcatgtttca gccattgatg ctttatgcgc
     9001 aaacatggac atcggaactg gtacagaggg atacaagact tagagactcc acgtttcact
     9061 ggcaccttcg gtgcaacaga tgtgtgaggc ccattgatga tataacactg gaaacttctc
     9121 agatcttcga gttcccggat gtgtcaaaaa ggatatccag gatggtttct ggagctgtcc
     9181 ctcaatttca gaagcttcct gatatccgtc taagaccagg tgattttgaa tccctaagtg
     9241 gtagagaaaa gtctcgccat atagggtcag ctcaggggct cttatactca atcttagtag
     9301 caattcacga ctcaggatac aatgatggga ccatcttccc tgtcaacata tacggcaaag
     9361 tttcccccag agactatttg agagggcttg caagagggat cttgataggg tcctcgattt
     9421 gcttcttgac acgaatgaca aatattaaca ttaacagacc tcttgaattg atctcagggg
     9481 taatttccta tattctcctg aggctggata atcatccctc tctgtatata atgcttagag
     9541 aaccgtctct tagaggagaa atattctcta tccctcagaa aatccccgcc gcttacccaa
     9601 ccactatgag agaaggcaac agatcgattt tgtgttacct ccaacacgtg ctacgctatg
     9661 agcgagaggc aatcacggcg tccccggaga atgactggct gtggatcttc tcagacttca
     9721 gaagtgtgaa aatgacgtac ttgaccctca ttacctacca gtctcacctc ctactccaga
     9781 gggttgagag aaacttgtct aagagtatga gagctactct gcgacaaatg agttccttaa
     9841 tgaggcaagt gctgggtggg cacggagaag ataccttgga gtcagacgat gacattcaac
     9901 gattactaaa agactctttg cgaaagacaa ggtgggtgga tcaagaggtg cgccatgcag
     9961 ctagaaccat gaatggagat tacagccccg acaagaaagt atcccacaag gcaggatgtt
    10021 cagaatgggt ctgctctgct caacagattg ccgtctccac ctcagccaac ccggcccctg
    10081 tctcagagct tgacattagg gccctctcta agaggtttca aaaccccttg atctcgggcc
    10141 tgagagtggt tcagtgggca accggtgccc attataagct taagcctatt ctagatgatc
    10201 taaatgtttt cccatctctc tgtcttgtaa ttggagacgg gtcaggggga atatcaaggg
    10261 cagttctcaa catgtttcca gattctaagc ttgtgttcaa cagcctattg gaggtgaatg
    10321 atctgatggc ttccggaaca catccactgc ctccttcagc aatcatgagt ggaggagatg
    10381 acatcatctc cagagtgata gactttgact caatatggga gaaaccgtcc gacctgagga
    10441 actcggccac ctggagatac ttccagtcgg ttcaaaaaca ggtcaacatg tcgtatgacc
    10501 tcattatttg tgatgcagaa gttactgata ttgcatctat caaccggata actctgttga
    10561 tgtctgattt cgcattgtct atagatggac cactttatct ggtcttcaaa acttacggga
    10621 ctatgctagt aaacccggac tataaagcta ttcaacatct gtcaagagcg ttcccttcgg
    10681 tcacagggtt tgtaacccaa gtaacttcat ccttttcttc tgagctatac ctccggttct
    10741 ctaaacgagg aaagtttttc agggacgccg agtacttgac ctcttccacc cttcgagaga
    10801 tgagccttgt gttgttcaat tgcagcagcc ccaaaagtga gatgcagaga gctcgttcct
    10861 taaactatca agacctggta aggggatttc ctgaagagat catatcaaat ccttacaacg
    10921 aaatgatcat aactctgatt gacaatgatg tagagtcctt cctagtccac aagatggtgg
    10981 atgatcttga gctacagagg ggaactctgt ctaaagtggc tatcattata tccatcatga
    11041 tcgttttttc caatagagtc ttcaacattt ccaaaccttt gactgacccc ttgttctacc
    11101 ccccatctga tcctaaaatc ctgaggcact tcaacatatg ttgcagtact atgatgtatc
    11161 tatctaccgc tttaggcgac gtccctaact tcgcaagact tcatgacctg tataacagac
    11221 ctataacttg ttacttcaga aaacaagtta ttcgagggaa tatttatcta tcttggagtt
    11281 ggtccgatga taccccagtg ttcaagagag tagcctgtaa ttctagcttg agtctgtcat
    11341 ctcactggat caggttgatc tacaagatag tgaagactac cagactcatt ggcagcataa
    11401 aagacctatc aggagaggta gaacgacatc ttcatgggta taacagatgg atcaccctcg
    11461 aggatatccg atctagatca tccctactag attacagttg tttgtaagcc ggatattacc
    11521 gaaagcctgt gcatgctaaa attcttgtat gatgcatctt gaaaaaaaca agatcttgaa
    11581 tccggacctc tggttgtttg attgtttttt ccatctttat tgtttttttg ttaagcgtgg
    11641 gtcggcatgg catctccacc tcctcgcggt ccgacctggg catccgaagg aggacgcacg
    11701 tccactcgga tggctaaggg agggcgggga tccggctgct aacaaagccc gaaaggaagc
    11761 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
    11821 ggtcttgagg ggttttttgc tgaaagtcgc gcttggcgta atcatggtca tagctgtttc
    11881 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11941 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12001 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12061 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12241 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    12901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    12961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    13021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    13081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    13141 ctggccccag tgctgcaatg ataccgcgag acccaccctt ggaggctcca gatttatcag
    13201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    13261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    13321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    13381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    13441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    13501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    13561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13621 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    13801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    13981 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
    14041 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    14101 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    14161 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    14221 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    14281 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    14341 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    14401 cctattggtt aaaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    14461 ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    14521 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    14581 gttgggtaac gcgagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
    14641 cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
    14701 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
    14761 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
    14821 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
    14881 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
    14941 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
    15001 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
    15061 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
    15121 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
    15181 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
    15241 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gattaagcgt
    15301 ctgatgagtc cgtgaggacg aaacccggcg taccgggtc
//
