LOCUS       sequence-343648-        9170 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 
            prime edit site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen
            C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, 
            Liu DR
  TITLE     Enhanced prime editing systems by manipulating cellular determinants
            of editing outcomes.
  JOURNAL   Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 
            10.1016/j.cell.2021.09.018.
  PUBMED    34653350
REFERENCE   2  (bases 1 to 9170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09"; journalName: "Cell"; date: "2021-10-28- 
            28"; volume: "184"; issue: "22"; pages: "5635-5652.e29"
COMMENT     Sequence Label: pPC1000
FEATURES             Location/Qualifiers
     source          1..9170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(6..110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(185..204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        376..755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        757..955
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     910..930
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             973..1153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1200..1325
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1822..2055
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2240..2284
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2582..2699
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2952..2985)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2989..3009
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3547..3758
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3759..4697
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4654..4674
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4718..5314
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4718..4737)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5214..5234
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5324..5377
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5390..6088
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(6108..6141)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6197..6785
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6250..6270)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6668..6679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6788..6804)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6789..6805)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7314..7494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7556..8144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7636..7655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8315..9106,9107..9170,1..5))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8938..8957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
       61 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      121 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
      181 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
      241 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
      301 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
      361 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      421 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      481 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      541 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      601 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      661 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      721 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      781 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      841 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      901 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
      961 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     1021 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1081 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1141 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     1201 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     1261 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1321 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1381 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1441 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1501 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1561 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1621 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     1681 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1741 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1801 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1861 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1921 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1981 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     2041 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgcggc
     2101 cgcttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     2161 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     2221 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     2281 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     2341 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2401 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2461 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2521 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     2581 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2641 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2701 gggtttatta cagggacagc agagatccag tttggttagt accgggcccg ctctagagat
     2761 ccgacgcgcc atctctaggc ccgcgccggc cccctcgcac ggacttgtgg gagaagctcg
     2821 gctactcccc tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa
     2881 tgtgcgataa aagacagata atctgttctt tttaatacta gctacatttt acatgatagg
     2941 cttggatttc tataacttcg tatagcatac attatacgaa gttataaaca gcacaaaagg
     3001 aaactcaccc taactgtaaa gtaattgtgt gttttgagac tataagtatc ccttggagaa
     3061 ccaccttgtt ggaccaggat gggcaccacc cgtttaagag ctaagctgga aacagcatag
     3121 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     3181 tttctcgagg cctttagtga tggcctggct cacctggaaa aacaacaatt aatgtgtttg
     3241 ttaccggtgt cacaggattt ctgggctcct acatccttgc agatttgtta ggacgttctc
     3301 caaagaacta catctgatag gcagcctgca ctggtggggt gaattctttg ccaaagtgat
     3361 aggccagcac acacccctct cgacaatatt ttgttgaacc gtacgaaaaa tttgcctcaa
     3421 atattaaagt tgtattaggc gatttatcta gcatgctacg tgcagcggcc gcctaggatc
     3481 catgccgata gcgttggttg agtggataac cgtattaccg ccaagcttat gcatgtgccc
     3541 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     3601 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     3661 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     3721 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     3781 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     3841 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     3901 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     3961 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     4021 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     4081 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     4141 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     4201 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     4261 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     4321 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     4381 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     4441 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgcgct
     4501 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     4561 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     4621 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     4681 tttttcttcc atttcaggtg tcgtgagcta gcccaccatg accgagtaca agcccacggt
     4741 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     4801 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     4861 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4921 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4981 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5041 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5101 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5161 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5221 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5281 gcgcacctgg tgcatgaccc gcaagcccgg tgccggatcg ggagagggca gaggaagtct
     5341 gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg gtcgccacca gcgagctgat
     5401 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
     5461 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     5521 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
     5581 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
     5641 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
     5701 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
     5761 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
     5821 gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
     5881 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
     5941 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
     6001 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
     6061 tagcaaactg gggcacaagc ttaattaaga attcgtcgag ggacctaata acttcgtata
     6121 gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga
     6181 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6241 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6301 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6361 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6421 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6481 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6541 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6601 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6661 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6721 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6781 cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta
     6841 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6901 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6961 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7021 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     7081 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     7141 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     7201 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     7261 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     7321 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7381 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7441 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatgt
     7501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7981 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
//
