LOCUS       sequence-345067-        5633 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-HKU1-nsp12
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2944..3043)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3060..3079)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3061..3079)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3084..3100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3084..3100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3531..4322
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4415..5003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4904..4923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5157..5174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5333..5360)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5452..5538)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5587..5609
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5601..5618
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5602..5618
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatcct ctaaagattt aaatttttta aaccgggttc ggggtactag tgtgaatgcc
      181 cggctagtac cctgtgctag tggtttatct actgatgttc aattaagggc atttgacatt
      241 tgtaatacca atagagctgg tataggttta tattataaag tgaattgttg ccgttttcag
      301 cgtatagatg acgacggtaa taaattggat aagttctttg ttgtcaaaag aactaattta
      361 gaagtttata ataaagagaa aacttattat gagttgacta aaagttgtgg tgttgtggct
      421 gaacatgatt tctttacatt tgatattgat ggtagtcgcg tgccacatat agttcgtagg
      481 aatctttcaa agtatactat gttagatctt tgctatgcat tgcgtcattt tgatcgtaat
      541 gattgttcaa tattgtgtga aattctttgt gagtatgctg attgtaaaga atcctacttt
      601 tctaagaaag attggtatga ttttgttgaa aatcctgata ttattaatat atataaaaaa
      661 ttaggcccta tttttaatag agctttactt aatactgtca tttttgcaga caccttagtt
      721 gaagtaggtt tagttggtgt tttaacttta gataaccaag atttgtatgg tcaatggtat
      781 gattttggtg attttataca aacagcccca gggtttggtg tggcagttgc agattcttac
      841 tattcttata tgatgcctat gttgactatg tgtcatgtat tagattgtga attatttgtt
      901 aatgatagtt atagacaatt cgatcttgta cagtatgatt ttactgatta caagttagag
      961 ttgtttaata agtattttaa gtattggggt atgaagtatc atcctaatac tgtggattgt
     1021 gataatgata ggtgtattat tcattgtgct aattttaata tactatttag tatggtttta
     1081 cctaatactt gttttggtcc ccttgttaga caaatttttg tagatggtgt accgtttgtt
     1141 gtttctattg gttaccatta caaagagtta ggtgtagtta tgaacttaga tgttgacaca
     1201 caccgttatc gtttgtctct taaagattta cttctttatg cagcagatcc tgctatgcac
     1261 gttgcatctg ctagtgctct gcttgattta cgaacttgtt gttttagtgt agctgccatt
     1321 acaagtggta taaaatttca aactgtaaaa ccaggtaact ttaaccaaga cttttacgag
     1381 tttgttaaaa gtaaaggctt gtttaaagag ggtagtacag ttgatttgaa acattttttc
     1441 tttactcaag atggtaatgc tgcaattact gattataatt attataagta taatttacct
     1501 actatggttg atattaagca gttattgttt gtattagaag ttgtttataa atattttgaa
     1561 atttatgatg gtggttgtat accagcatca caagttattg ttaataatta tgataaaagt
     1621 gctggttatc catttaataa atttggtaaa gccagacttt attatgaggc attatcattt
     1681 gaggaacaga atgaaattta tgcatatact aaacgtaatg ttctgcccac cttaactcaa
     1741 atgaatttaa aatatgctat cagtgctaag aatagagctc gcactgtagc aggtgtttct
     1801 attcttagta ctatgacagg ccgaatgttc catcaaaaat gtttgaagag tatagcagct
     1861 acccgaggtg ttcctgttgt tataggaacc actaaatttt atggtggttg ggacgatatg
     1921 ttacgtcatc ttataaagga tgttgacaac cctgttctta tgggttggga ttatcctaaa
     1981 tgtgatcgtg ctatgccaaa tattttgcgt attgttagta gtttagtttt ggcccgcaaa
     2041 catgaatttt gttgttcaca tggtgataga ttttatcgcc ttgcgaatga atgtgctcaa
     2101 gttttgagtg aaatagttat gtgtggcggt tgctattatg ttaagcctgg tggtactagc
     2161 agtggtgatg caactactgc ttttgctaat tctgttttta atatatgtca ggctgttact
     2221 gctaatgttt gttctcttat ggcctgtaat ggccataaga ttgaagattt aagtatacgc
     2281 aatttacaaa aacgcttata ctctaatgtt tatcgtacag attatgttga ttatacattt
     2341 gttaatgagt attatgaatt tttatgtaag cattttagta tgatgatttt gagtgatgat
     2401 ggtgttgtct gttataactc tgattatgct agtaagggtt atatagctaa tataagtgtt
     2461 tttcaacaag ttttgtacta tcagaataat gtctttatgt ctgaatctaa atgttgggtt
     2521 gaaaatgata ttactaatgg tcctcatgaa ttttgttccc aacatactat gttagttaag
     2581 atagatggtg attatgttta tttaccatat ccagatcctt ctagaatttt aggagctggt
     2641 tgttttgttg atgatttatt gaagactgac agtgttcttt tgatagagcg ctttgtaagt
     2701 ctagctatag atgcttaccc tttagtacat catgaaaatg aagaatacca aaaagtcttt
     2761 cgtgtatatt tagaatatat aaaaaaactg tataatgatc ttggtactca gatcttagat
     2821 agttatagtg ttattttaag tacttgtgat ggtttaaagt ttactgaaga atcattttac
     2881 aagaatatgt atttaaaaag tgccgtgatg cagcttaatt aagacgcgat cgcggcctca
     2941 tgggcccagc tttcttgtac aaagttggca ttataaaaaa taattgctca tcaatttgtt
     3001 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     3061 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     3121 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     3181 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     3241 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     3301 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3361 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     3421 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3481 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     3541 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     3601 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3661 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3721 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3781 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3841 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3901 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3961 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     4021 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     4081 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     4141 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     4201 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     4261 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     4321 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4381 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4441 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4501 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4561 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4621 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4681 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4741 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4801 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4861 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4921 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4981 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5041 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5101 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5161 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5221 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5281 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     5341 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     5401 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     5461 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     5521 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     5581 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccc
//
