LOCUS       sequence-345224-        6780 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pWSK29 with S. flexneri wzzpHS2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Murray GL, Attridge SR, Morona R
  TITLE     Altering the length of the lipopolysaccharide O antigen has an 
            impact on the interaction of Salmonella enterica serovar Typhimurium
            with macrophages and complement.
  JOURNAL   J Bacteriol. 2006 Apr;188(7):2735-9. doi: 
            10.1128/JB.188.7.2735-2739.2006.
  PUBMED    16547065
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.188.7.2735-2739"; journalName: "J Bacteriol"; date: 
            "2006-04"; volume: "188"; issue: "7"; pages: "2735-9"
COMMENT     Sequence Label: pWSK29-wzzpHS2
FEATURES             Location/Qualifiers
     source          1..6780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(32..823,824..892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     655..674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(893..997)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1023..1478)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1160..1181)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1372..1391
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1605..1627
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1619..1636
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1620..1636
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1646..1665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1646..1664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1689..1705
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1690..1706
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3086..3102)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3086..3102)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3139..3159)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3139..3157)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3178..3194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3178..3194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3191..3213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3202..3218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3226..3232,3233..3250,3251..3256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3271..3292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3409..3426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      4571..4793
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             4841..5791
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
       61 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
      121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
      181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
      241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
      301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
      361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccagcga tcaaggcgag
      421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
      481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
      541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
      601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
      661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
      721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
      781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1021 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     1081 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     1141 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     1201 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     1261 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     1321 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     1381 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     1441 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     1501 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1561 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1621 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
     1681 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc atagctatgc
     1741 agtgagctga aagcgatcct gacgcatttt tccggtttac cccggggaaa acatctcttt
     1801 ttgcggtgtt tgcggcagaa tcgcgttcag cgcgttttag cggtgcgcgc aataaaatgt
     1861 tatgttaaat gtctgctgaa tggtttttag gttttttcct aacatatgat agaataaaaa
     1921 aaccatatgt taatatattg tgtttttttc aatgaaaatc tcaaaggtct ctgacgaaca
     1981 tttttctaat tacaacatgt tgccgaatgg taatgaagaa attgaccttt ttggcttgat
     2041 tagcgtttta tgggcagcaa aaataaaaat tgttggggtg attctatttt ttgcttgtat
     2101 tggctttctt gtttcttttg ttttgccaca aaaatggact agttctgctg tggttactcc
     2161 tgctgagtct gttcaatgga gtgaattaaa taaggagctt tccaagcttc atgttctggg
     2221 agtggatttt gatatcaatc gtgatagcgc atttgctctg tttattaaga aatttcagtc
     2281 cgttaattcg ttgaatgagt atatgagatc atctccttat gttatggaga ttattaaaaa
     2341 gaataacatt agcgctctcg aactgcatcg tgcaattgtt gagttgagta aaaatatgaa
     2401 gtcgattgat gataatgcca gtaagaaaaa cgacaagtca tcattgtatg tatcatggac
     2461 tctgagtttt actgctccaa caagtaaaga agctcacgat gtgttgtctg ggtatattaa
     2521 ttatgtttct tcccttgttg taagggattt gatggaagat ataagaaata aactagaagt
     2581 taaaactaat gttgaaaaag aaattcttgc actggatgag ataaaaatta gaaaccagct
     2641 gaatgcagat attcgacgcc tcaattattc actggaggtt gctaatgcgg ctggaataaa
     2701 aaaacctgta tacagcaatg gtcagattat gaaggatgac ccagattttc ctgtggctct
     2761 cggttctgat ggtatagcaa ctaaattgaa catcaaaaaa tcaatcaagg atgtttcgga
     2821 attgagtggg gagttgcgaa atcgtcaata tgttgtgaat caattggttg tggcgaaagt
     2881 gggggatgtt gattttatgc catttcagta tcaattgagc ccaacgttac cggtcagaaa
     2941 ggatgggcca agtaatgcca taattgtaat tctttcttct gtcattggag ggatgcttgc
     3001 atgtggctat atacttttac gtcatgccat ggaggccaga aagatgagtg tgattgagta
     3061 aaagttgtgc tgatgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg
     3121 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     3181 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3241 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3301 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagatc tctacgggtc
     3541 ggatttgaag tcgtcttggt aggaggcagc ctgaatggcg aatgccgatg cccttgagag
     3601 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     3661 tgactgtctt ctttatcatg caactcgtag gacagggtgc cggcagcgct ctgggtcatt
     3721 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     3781 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     3841 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     3901 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     3961 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     4021 cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc
     4081 acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     4141 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     4201 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccgcag acccgccata
     4261 aaacgccctg agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga
     4321 atccataaaa ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca
     4381 gcgaactgaa tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg
     4441 aatattcagc gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg
     4501 tctgttttgt agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga
     4561 ctaaagtagt gagttataca cagggctggg atctattctt tttatctttt tttattcttt
     4621 ctttattcta taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc
     4681 ggaatttaca gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt
     4741 acagataccc acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa
     4801 ttggtatagt gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt
     4861 caaagcaaat gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat
     4921 tttatgctgt gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac
     4981 ggtatcgttc acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta
     5041 tggtgtatta gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc
     5101 tttggttaaa ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa
     5161 attagaatta gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat
     5221 aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga
     5281 gtggttatta aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct
     5341 tgatgaattt aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa
     5401 ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt
     5461 ggttgataag cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca
     5521 aatggatctc gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc
     5581 aacaaccatt acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc
     5641 tttaactgca aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag
     5701 taagtatgat ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga
     5761 gaacatactg gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg
     5821 aagcatcaag actaacaaac aaaagtagaa caactgttca ccgttacata tcaaagggaa
     5881 aactgtccat atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac
     5941 gagtttttgg tgcattcaaa gctgttcacc atgaacagat cgacaatgta acagatgaac
     6001 agcatgtaac acctaataga acaggtgaaa ccagtaaaac aaagcaacta gaacatgaaa
     6061 ttgaacacct gagacaactt gttacagctc aacagtcaca catagacagc ctgaaacagg
     6121 cgatgctgct tatcgaatca aagctgccga caacacggga gccagtgacg cctcccgtgg
     6181 ggaaaaaatc atggcaattc tggaagaaat agcgctttca gccggcaaac cggctgaagc
     6241 cggatctgcg attctgataa caaactagca acaccagaac agcccgtttg cgggcagcaa
     6301 aacccgtact tttggacgtt ccggcggttt tttgtggcga gtggtgttcg ggcggtgcgc
     6361 gcaagatcca ttatgttaaa cgggcgagtt tacatctcaa aaccgcccgc ttaacaccat
     6421 cagaaatcct cagcgcgatt ttaagcacca accccccccc gtaacaccca aatccatact
     6481 gaaagtggct ttgttgaata aatcagattt cgggtaagtc tcccccgtag cgggttgtgt
     6541 tttcaggcaa tacgcacgct ttcaggcata cctgctttcg tcattttgtt cagcgctcgt
     6601 accagggcca tagcctccgc aacctgacca tcgtagtcac gcagcgtcag tgaacccccg
     6661 aacagagatc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6721 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
//
