LOCUS       sequence-345256-       11481 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Drosophila transgene expression under UAS/Gal4 control, PhiC31 
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11481)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_UAS>Gateway
FEATURES             Location/Qualifiers
     source          1..11481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4789..4822
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4835..5265
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5311..5405
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5439..5533
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5567..5661
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5695..5789
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6004..6128
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6153..6158,6159..6176,6177..6183)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6237..6896
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6303..6322)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7238..7543
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7422..7441
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7584..7708)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    8405..8539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8442..8461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8496..8515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8541..8971
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    9005..9074
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9293..9311)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9332..9348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9332..9348)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9345..9367)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9356..9372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9380..9386,9387..9404,9405..9410))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9425..9446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9563..9580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9734..10322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9814..9833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10493..11284,11285..11353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11116..11135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11354..11458)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa
     4381 cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt
     4441 tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg
     4501 tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt
     4561 ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg
     4621 ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa
     4681 aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga
     4741 atacatttaa tttagaaaat gcttggattt cactggaact aggctagcat aacttcgtat
     4801 aatgtatgct atacgaagtt atgctagcgg atcctggcca cgtaataagt gtgcgttgaa
     4861 tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca taccttatca
     4921 aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc atacccgttt
     4981 ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt tgacgaaaca
     5041 aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt taataaaata
     5101 cattgcatac cctcttttaa taaaaaatat tgcatacgtt gacgaaacaa attttcgttg
     5161 catacccaat aaaagattat tatattgcat accttttctt gccataccat ttagccgatc
     5221 aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacagatc caagctggcc
     5281 gcggctcgat aagcttgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt
     5341 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
     5401 ccgagcggag actctagccc tagggcatgc ctgcaggtcg gagtactgtc ctccgagcgg
     5461 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     5521 actgtcctcc gagcggagac tctagcgcta gcgcatgcct gcaggtcgga gtactgtcct
     5581 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     5641 agcggagtac tgtcctccga gcggagactc tagcgctagc gcatgcctgc aggtcggagt
     5701 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     5761 gtcctccgag cggagtactg tcctccgagc ggagactcta gcgacgtcga gactctagcg
     5821 agctcgcccg gggatcgagc gcagcggtat aaaagggcgc ggggtggctg agagcatcag
     5881 ttgtgaatga atgttcgagc cgagcagacg tgccgctgcc ttcgttaata tcctttgaat
     5941 aagccaactt tgaatcacaa gacgcatacc aaacgaattc gcagatctta attaaccggt
     6001 accacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     6061 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     6121 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     6181 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     6241 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     6301 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     6361 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     6421 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     6481 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     6541 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     6601 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     6661 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     6721 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     6781 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     6841 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaacgc
     6901 gtggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     6961 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa
     7021 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     7081 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     7141 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     7201 aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta
     7261 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     7321 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     7381 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     7441 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     7501 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat
     7561 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     7621 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     7681 ctcgttcagc tttcttgtac aaagtggtga tatcgcatgc ggccgcggcg cgcctctaga
     7741 aatgaatcgt ttttaaaata acaaatcaat tgttttataa tattcgtacg attctttgat
     7801 tatgtaataa aatgtgatca ttaggaagat tacgaaaaat ataaaaaata tgagttctgt
     7861 gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt gcaaataaac ccatgattat
     7921 ttgattaaaa ttgttgtttt ctttgttcat agacaatagt gtgttttgcc taaacgtgta
     7981 ctgcataaac tccatgcgag tgtatagcga gctagtggct aacgcttgcc ccaccaaagt
     8041 agattcgtca aaatcctcaa tttcatcacc ctcctccaag tttaacattt ggccgtcgga
     8101 attaacttct aaagatgcca cataatctaa taaatgaaat agagattcaa acgtggcgtc
     8161 atcgtccgtt tcgaccattt ccgaaaagaa ctcgggcata aactctatga tttctctgga
     8221 cgtggtgttg tcgaaactct caaagtacgc agtcaggaac gtgcgcgaca tgtcgtcggg
     8281 aaactcgcgc ggaaacatgt tgttgtaacc gaacgggtcc catagcgcca aaaccaaatc
     8341 tgccagcgtc aatagaatga gcacgatgcc gacaatggag ctggcttgga tagcgattcg
     8401 agttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8521 atcttatcat gtctggatct tggccacgta ataagtgtgc gttgaattta ttcgcaaaaa
     8581 cattgcatat tttcggcaaa gtaaaatttt gttgcatacc ttatcaaaaa ataagtgctg
     8641 catacttttt agagaaacca aataattttt tattgcatac ccgtttttaa taaaatacat
     8701 tgcataccct cttttaataa aaaatattgc atactttgac gaaacaaatt ttcgttgcat
     8761 acccaataaa agattattat attgcatacc cgtttttaat aaaatacatt gcataccctc
     8821 ttttaataaa aaatattgca tacgttgacg aaacaaattt tcgttgcata cccaataaaa
     8881 gattattata ttgcatacct tttcttgcca taccatttag ccgatcaatt gtgctcggca
     8941 acagtatatt tgtggtgtgc caaccaacaa cggatccact agtgtcgacg atgtaggtca
     9001 cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact
     9061 ccacctcacc catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg
     9121 cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg
     9181 tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt
     9241 tctcgacggt cacggcgggc atgtcgacac tagttctagc cagcttttgt tccctttagt
     9301 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9361 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9421 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9481 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10381 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10501 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10561 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10621 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10681 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10741 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10801 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10861 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10921 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10981 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11041 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11101 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11161 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11221 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11281 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11341 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11401 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11461 catttccccg aaaagtgcca c
//
