LOCUS       sequence-345472-        5750 bp    DNA     circular SYN 15-APR-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1351:Dendra
FEATURES             Location/Qualifiers
     source          1..5750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     342..361
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        342..360
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1990..2013
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2270..3070
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3195..3783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3684..3703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc
       61 cgccagaaat ctagacggtg accacaacgc gcccgctttg atcggggacg tcgaaatatt
      121 ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg atgatcggcc
      181 gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc tgtgcgttcg
      241 cacgcacagg cccggtgtga gaagggtctc tgacgagcgg gagaactccc tatcagtgat
      301 agagtttgtc ctccctatca gtgatagata ggcttaagga gtaatacgac tcactatagg
      361 gagaaggagg ccattatgac caagcagccc ggaaagctac aaccacactt cgacgaggtc
      421 caagctcatt acgacctgtc ggacgacttc ttcgcgctgt tcctcgaccc gtcgagaacc
      481 tacagctgcg cgtatttcga gcgcgacgac atgacgctgg aagaagccca gctcgccaag
      541 atcgacctgt cgctgggcaa gctgggcctg cagccgggga tgaccctgct cgatgtcgga
      601 tgcggttggg gtgcaacgat gttgcgcgca ctcgagcgct atgacgtcaa cgtcgtcggg
      661 ctgacactga gccgcaatca gcaggcgcac gtcgagcgcc gcttctccga gtcatccagt
      721 ccccgcacca agcgcgtgct gctcgcgggc tgggaggagt tcgacgaacc ggtggaccgc
      781 atcgtgtcga tcggagcgtt cgagcatttc ggtcacgagc ggtacgacga cttcttccgg
      841 ttcgcgcatc gcgtgctacc cgacgacggc gtgatgctgt tgcacaccat cacgggcatc
      901 cacccgaagc acgcgcagga gctgggtgtg ccgctgacct tcgagatggc gcggttcatc
      961 aggttcatcg tcaccgagat cttccccggt gggcggctgc cgaccgtcga catggtgatc
     1021 gaccacgcga cccgcgcgtc gttcacgctg gaacgcaggc agtcgctgca actgcattac
     1081 gcccgcacgc tggacatgtg ggccgccgcg ctcgaatccc accacgacga tgctgtgcga
     1141 atccagtcgc aggaggtcta cgaccggtac atgcactacc tcacgggttg cgccaacgcg
     1201 ttccgcgtcg gctatatcga cgtcaaccag ttcaccctgg cgaagaagct tggtgcggcc
     1261 gcgggcaaca cgcctggcat caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg
     1321 gagggcaacg tgaacggtca cgccttcgtg atcgaggggg agggtaaggg taagccttac
     1381 gaagggaccc agaccgccaa cctcaccgtc aaggagggcg cccctctccc attctcgtac
     1441 gatatcctca ctaccgcggt gcattacggg aaccgcgtgt tcaccaagta ccccgaggat
     1501 atccctgact acttcaagca gagcttcccg gagggatact cctgggagcg gacgatgacc
     1561 ttcgaagaca agggtatctg tacgatccgc agcgacatct cgctggaagg ggactgtttc
     1621 ttccagaacg tccgtttcaa ggggaccaac tttccgccga acggcccggt catgcagaag
     1681 aagaccctca agtgggagcc gagcacggag aagctgcacg ttcgggacgg cctcctggtc
     1741 ggcaacatca acatggcgct gctgctggaa ggcggcggcc actacctatg cgacttcaag
     1801 accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc
     1861 atcgaaatcc tggggaacga ttcggactac aacaaggtca agttgtacga gcacgctgtg
     1921 gcacgctata gcccgctccc gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg
     1981 tatttccagg actacaagga cgacgacgac aagtgattta aataacccct tggggcctct
     2041 aaacgggtct tgaggggttt tttgttaact agcgtacgat cgaattcctg cagctcacgg
     2101 taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa
     2161 tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat
     2221 caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc
     2281 ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct
     2341 caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg
     2401 gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctacgc tgggtccggg
     2461 agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac
     2521 gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt
     2581 aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat
     2641 ctctgatcca ttgcccctgc caccttactc gcctgcaagc ccggtcgccc gtgtccatga
     2701 actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct
     2761 tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga
     2821 tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt
     2881 ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt
     2941 tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga
     3001 tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca
     3061 gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct
     3121 gattgctggg gcaacacggg ggatccacta gttccatgag cgtcagaccc cgtagaaaag
     3181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3301 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3601 acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3901 gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg
     3961 ggatcctcta gagtcgacca ccaagggcac catctctgct tgggccaccc cgttggccgc
     4021 agccagctcg ctgagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt
     4081 tccgaccgct gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc
     4141 tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt
     4201 cagcatcgaa agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga
     4261 gtcgcacaag agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct
     4321 gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc
     4381 tgccgaccac atatgggccg gtcaagatag gtttttaccc cctctcggct gcatcctcta
     4441 agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc acaggagttg
     4501 caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc ggatggggat
     4561 cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg caggacggtg
     4621 tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc tggctcgcgg
     4681 gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg gcgaagaagg
     4741 cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg gagcgcgacc
     4801 tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc
     4861 taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg tgggccggga
     4921 tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg gcggtgatga
     4981 acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag cagaaggcag
     5041 ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga
     5101 tcttcgagca ctaccggatc gcggcataca tcctggcgtg gacgagcctc cggttcggag
     5161 agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg aagctccggg
     5221 tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc
     5281 ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga gcgcacatga
     5341 aggaccgtac gaagatgaac aagggccccg aggcattcct ggtgaccacg acgcagggca
     5401 accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc
     5461 ggccggaact ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag
     5521 gtgcgacgac caaggagctg atggcccgtc tcggtcacac gactcctagg atggcgatga
     5581 agtaccagat ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg
     5641 ccaagacctc ctgaaacgca aaaagccccc ctcccaagga cactgagtcc taaagagggg
     5701 ggtttcttgt cagtacgcga agaaccacgc ctggccgcga gcgccagcac
//
