LOCUS       sequence-345596-        5765 bp    DNA     circular SYN 15-APR-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1134:Dendra
FEATURES             Location/Qualifiers
     source          1..5765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     739..758
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        739..757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2402..2425
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2682..3482
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3607..4195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4096..4115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatgaacaag ggccccgagg cattcctggt gaccacgacg cagggcaacc ggctgtcgaa
       61 gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag atcggtcggc cggaactccg
      121 catccacgac ctccgcgctg tcggcgctac gttcgccgct caggcaggtg cgacgaccaa
      181 ggagctgatg gcccgtctcg gtcacacgac tcctaggatg gcgatgaagt accagatggc
      241 gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc aagctggcca agacctcctg
      301 aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa agaggggggt ttcttgtcag
      361 tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc cgctctgtgc ggagacctgg
      421 gcaccagccc cgccgccgcc aggagcattg ccgttcccgc cagaaatcta gacggtgacc
      481 acaacgcgcc cgctttgatc ggggacgtcg aaatattgga tcgtcggcac cgtcacggcc
      541 gtgggaggcg gcacgatccg cgacgtgatg atcggccgca tccccacggt gctgcgcagt
      601 gagctctacg ccatcccggc gttgatctgt gcgttcgcac gcacaggccc ggtgtgagaa
      661 gggtctctga cgagcgggag aactccctat cagtgataga gtttgtcctc cctatcagtg
      721 atagataggc ttaaggagta atacgactca ctatagggag aaggaggcca ttatgacgtg
      781 gaatccgcac gcgaatcggc tcaagacatt cctgctgctc gtgggcatgt ccgggctcat
      841 cgtgttcgtc gggtcgctgt tcggccgcaa cgtgatgttc cttgcggtgt tgttcgccgt
      901 cggcatgaac gcctacgtct acttcaacag cgacaagctg gcgctgcggg ccatgcacgc
      961 acagcccatc accgaggtgc aggcgccgga gatctaccgg atcgtccgtg aactcgcgac
     1021 caccgcgcac cagcccatgc cgcggctcta catctccgac accgccaacc cgaacgcgtt
     1081 cgcgacgggt cggaaccccc gcaatgccgc ggtgtgctgt accaccggca tcctgcagtt
     1141 gctcaacgaa cgtgaactgc gtgccgtgct cggccacgag ctctcgcacg tctacaaccg
     1201 cgacatcctg atctcgtgcg tcgcaggcgc gatggcgtcg gtgatcaccg cgctggccaa
     1261 catggcgatg ttcgcaggca tgttcggcgg taaccgcgaa ggtggcaatc cgcttgccct
     1321 gcttctggtt tcgttcctcg gcccgatcgc ggccacggtc gtcaagctcg ccgtctcccg
     1381 ctcgcgcgag taccaggccg accagtccgg cgccgagctg accggtgacc ccctcgcact
     1441 ggcatccgcc ctgcgcaaga tctccggtgg agtcgaggcg gccccgctgc ccccgcagcc
     1501 ccagctcgcc gatcaggccc acctgatgat cgccagcccg ttccgtgccg gcgagcgcat
     1561 cggcaagctc ttctcgaccc acccgccgat cgccgaccgc atccgccggc tcgaggacat
     1621 ggccggccgt ggcggctccc gcccggactc gtactacaag cttggtgcgg ccgcgggcaa
     1681 cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata tggagggcaa
     1741 cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt acgaagggac
     1801 ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt acgatatcct
     1861 cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg atatccctga
     1921 ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga ccttcgaaga
     1981 caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt tcttccagaa
     2041 cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga agaagaccct
     2101 caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg tcggcaacat
     2161 caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca agaccacgta
     2221 caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc gcatcgaaat
     2281 cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg tggcacgcta
     2341 tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc tgtatttcca
     2401 ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct ctaaacgggt
     2461 cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac ggtaactgat
     2521 gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc
     2581 tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg
     2641 actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca gcggtggagt
     2701 gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg
     2761 gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg
     2821 aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg ggagggacgc
     2881 tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg
     2941 ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat gtaaagcgca
     3001 gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc atctctgatc
     3061 cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg
     3121 ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat cttgccgagt
     3181 tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag gatggcaagt
     3241 tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc ttggcggaca
     3301 ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc tttgctcggt
     3361 tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga gatccgttga
     3421 tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg
     3481 gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg ctgattgctg
     3541 gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa agatcaaagg
     3601 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3661 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3721 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3781 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3841 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3961 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4021 cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4081 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4141 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4201 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4261 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4321 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc
     4381 tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct
     4441 cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg
     4501 ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct
     4561 ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg
     4621 aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca
     4681 agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg
     4741 gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc
     4801 acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
     4861 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
     4921 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
     4981 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
     5041 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
     5101 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
     5161 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
     5221 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
     5281 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
     5341 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
     5401 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     5461 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     5521 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     5581 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     5641 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     5701 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     5761 acgaa
//
