LOCUS       sequence-345599-        5585 bp    DNA     circular SYN 15-APR-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1048:Dendra
FEATURES             Location/Qualifiers
     source          1..5585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1065..1088
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1345..2145
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2270..2858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2759..2778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5167..5186
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5167..5185
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgccaagtt cggcgccacc gagatcctgg cggggctggc accgcgcggc ggcggcgggg
       61 tacgcctggc ggacgccatc gggaccagca aggccaagga gctcgtgttc agcggccggt
      121 tcgtcggcgc cgaggaagcg ctcgagatcg gcctggtcga cgagatggtg gcacccgacc
      181 acgtctacga ggccgcgctc gcgtgggccc gccggttctc cgaccatccg gtcgacgtgc
      241 tggccgccgc gaaagcctcc ctcaacgggc cggtcaactg gcggggcccc cgcccgaccg
      301 atcggatggt ggaccggccc aagcttggtg cggccgcggg caacacgcct ggcatcaatc
      361 tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct
      421 tcgtgatcga gggggagggt aagggtaagc cttacgaagg gacccagacc gccaacctca
      481 ccgtcaagga gggcgcccct ctcccattct cgtacgatat cctcactacc gcggtgcatt
      541 acgggaaccg cgtgttcacc aagtaccccg aggatatccc tgactacttc aagcagagct
      601 tcccggaggg atactcctgg gagcggacga tgaccttcga agacaagggt atctgtacga
      661 tccgcagcga catctcgctg gaaggggact gtttcttcca gaacgtccgt ttcaagggga
      721 ccaactttcc gccgaacggc ccggtcatgc agaagaagac cctcaagtgg gagccgagca
      781 cggagaagct gcacgttcgg gacggcctcc tggtcggcaa catcaacatg gcgctgctgc
      841 tggaaggcgg cggccactac ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg
      901 tccagctgcc cgacgcgcac ttcgtggacc accgcatcga aatcctgggg aacgattcgg
      961 actacaacaa ggtcaagttg tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc
     1021 aggtctggtt cgaaggcggc ggcggggaga acctgtattt ccaggactac aaggacgacg
     1081 acgacaagtg atttaaataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
     1141 taactagcgt acgatcgaat tcctgcagct cacggtaact gatgccgtat ttgcagtacc
     1201 agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt
     1261 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     1321 cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac
     1381 gaatggcgaa aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt
     1441 gtgctgctgg tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga
     1501 ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg
     1561 tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga
     1621 gttgtctctg acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc
     1681 tttgcggcag cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct
     1741 tactcgcctg caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc
     1801 ggcgtgggac acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc
     1861 tatggggtgc cgagacactg caccattctt caggatggca agttggtacg cgtcgattat
     1921 ctcgagaatg accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag
     1981 agccttcaga aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac
     2041 attgtggcga cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca
     2101 gaggcgggat gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg
     2161 agaacgagat gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgggggatc
     2221 cactagttcc atgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2281 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2341 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2401 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2461 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2521 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2581 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2641 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg
     2701 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2761 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2821 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2881 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2941 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3001 cgaccgagcg caacgcgtgc ggccgcggta cccggggatc ctctagagtc gaccaccaag
     3061 ggcaccatct ctgcttgggc caccccgttg gccgcagcca gctcgctgag agccgtgaac
     3121 gacagggcga acgccagccc gccgacggcg agggttccga ccgctgcaac tcccggtgca
     3181 accttgtccc ggtctattct cttcactgca ccagctccaa tctggtgtga atgcccctcg
     3241 tctgttcgcg caggcggggg gctctattcg tttgtcagca tcgaaagtag ccagatcagg
     3301 gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc acaagagttg cagacccctg
     3361 gaaagaaaaa tggccagagg gcgaaaacac cctctgacca gcggagcggg cgacgggaat
     3421 cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg accacatatg ggccggtcaa
     3481 gataggtttt taccccctct cggctgcatc ctctaagtgg aaagaaattg caggtcgtag
     3541 aagcgcgttg aagcctgaga gttgcacagg agttgcaacc cggtagcctt gttcacgacg
     3601 agaggagacc tagttggcac gtcgcggatg gggatcgctg aagactcagc gcagcgggag
     3661 gatccaagcc tcatacgtca acccgcagga cggtgtgagg tactacgcgc tgcagaccta
     3721 cgacaacaag atggacgccg aagcctggct cgcgggcgag aagcggctca tcgagatgga
     3781 gacctggacc cctccacagg accgggcgaa gaaggcagcc gccagcgcca tcacgctgga
     3841 ggagtacacc cggaagtggc tcgtggagcg cgacctcgca gacggcacca gggatctgta
     3901 cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt gaagtggcgg tcacagagat
     3961 gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc cgactgcccg
     4021 ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg acaagctgat
     4081 cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct
     4141 gacgcctgag gagctggaca tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc
     4201 atacatcctg gcgtggacga gcctccggtt cggagagctg atcgagcttc gccgcaagga
     4261 catcgtggac gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg
     4321 gaacaagatc gtcgttggca acgccaagac cgtccggtcg aagcgtcctg tgacggttcc
     4381 gcctcacgtc gcggagatga tccgagcgca catgaaggac cgtacgaaga tgaacaaggg
     4441 ccccgaggca ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac
     4501 caagtcgctg aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct
     4561 ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc
     4621 ccgtctcggt cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg
     4681 cgacgaggct atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag
     4741 cccccctccc aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac
     4801 cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg
     4861 ccgccgccag gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg
     4921 ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc
     4981 acgatccgcg acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc
     5041 atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg
     5101 agcgggagaa ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt
     5161 aaggagtaat acgactcact atagggagaa ggaggccatt atgaacgagt tcatcaagat
     5221 cgtcctcggt tccgcgcccg agcagacggg cgtggccacg ttgctgttgt cgcggccccc
     5281 gacgaacgcg ttgacccgcc agatgtaccg cgagatctcc atcgccgcga acgagctcgc
     5341 ccagcgcgcc gacgtgtcgt cggtcatcgt ctacggcggc cacgagatct tctgcgcggg
     5401 cgacgacatc cccgaactgc gcaccctcga cgccgaagag accgcggcgg ccgaccacgc
     5461 gctgcagcgc tgcatcgagg ccgttgccgc catccccaaa cccacggtcg cggccgtcac
     5521 cgggtacgcg ctcggcagcg ggatgaacct ggcgctggcc gcggactggc gggtgagcgg
     5581 cgaca
//
