LOCUS       sequence-345603-        8725 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura 
            T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, 
            Meyerson M
  TITLE     Allele-dependent variation in the relative cellular potency of 
            distinct EGFR inhibitors.
  JOURNAL   Cancer Biol Ther. 2007 May . 6(5):661-7.
  PUBMED    17495523
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Biol
            Ther. 2007 May . 6(5):661-7."
COMMENT     Sequence Label: pBabe EGFR(L858R/T790M)
FEATURES             Location/Qualifiers
     source          1..8725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             join(1379..1450,1451..3313,3314..3382,3383..5011)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLIMQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGRAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     primer_bind     complement(5048..5068)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5053..5382
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5233..5368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5392..5991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5392..5411)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5888..5908
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6212..6681
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6811..6828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6982..7570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7062..7081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7730..8521,8522..8590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8591..8695)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacatcat
     1381 gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc tctgcccggc
     1441 gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc tcacgcagtt
     1501 gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact gtgaggtggt
     1561 ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct tcttaaagac
     1621 catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc gaattccttt
     1681 ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg ccttagcagt
     1741 cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga gaaatttaca
     1801 ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca acgtggagag
     1861 catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga tggacttcca
     1921 gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga gctgctgggg
     1981 tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc agtgctccgg
     2041 gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg caggctgcac
     2101 aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag ccacgtgcaa
     2161 ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg atgtgaaccc
     2221 cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta attatgtggt
     2281 gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga tggaggaaga
     2341 cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta acggaatagg
     2401 tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa
     2461 ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt
     2521 cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat
     2581 cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga
     2641 gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt
     2701 cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt
     2761 gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt
     2821 tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc
     2881 cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag
     2941 ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct
     3001 tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga
     3061 gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca
     3121 gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg
     3181 agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca
     3241 tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc
     3301 taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc
     3361 cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca cgctgcggag
     3421 gctgctgcag gagagggagc ttgtggagcc tcttacaccc agtggagaag ctcccaacca
     3481 agctctcttg aggatcttga aggaaactga attcaaaaag atcaaagtgc tgggctccgg
     3541 tgcgttcggc acggtgtata agggactctg gatcccagaa ggtgagaaag ttaaaattcc
     3601 cgtcgctatc aaggaattaa gagaagcaac atctccgaaa gccaacaagg aaatcctcga
     3661 tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc tgggcatctg
     3721 cctcacctcc accgtgcaac tcatcatgca gctcatgccc ttcggctgcc tcctggacta
     3781 tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt gtgtgcagat
     3841 cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc tggcagccag
     3901 gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttggga gggccaaact
     3961 gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta tcaagtggat
     4021 ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct ggagctacgg
     4081 ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa tccctgccag
     4141 cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca tatgtaccat
     4201 cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc gcccaaagtt
     4261 ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct accttgtcat
     4321 tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct accgtgccct
     4381 gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca tcccacagca
     4441 gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc tgagtgcaac
     4501 cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct gtcccatcaa
     4561 ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga ctgaggacag
     4621 catagacgac accttcctcc cagtgcctga atacataaac cagtccgttc ccaaaaggcc
     4681 cgctggctct gtgcagaatc ctgtctatca caatcagcct ctgaaccccg cgcccagcag
     4741 agacccacac taccaggacc cccacagcac tgcagtgggc aaccccgagt atctcaacac
     4801 tgtccagccc acctgtgtca acagcacatt cgacagccct gcccactggg cccagaaagg
     4861 cagccaccaa attagcctgg acaaccctga ctaccagcag gacttctttc ccaaggaagc
     4921 caagccaaat ggcatcttta agggctccac agctgaaaat gcagaatacc taagggtcgc
     4981 gccacaaagc agtgaattta ttggagcatg agatgtagga attcgccagc acagtggtcg
     5041 accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5101 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5161 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5221 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5281 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5341 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac catgaccgag
     5401 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5461 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5521 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5581 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5641 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5701 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5761 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5821 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5881 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5941 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     6001 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat aaaataaaag
     6061 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     6121 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     6181 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     6241 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     6301 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     6361 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     6421 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     6481 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     6541 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     6601 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     6661 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
     6721 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cgggtagagg
     6781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7621 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7681 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8341 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8701 ttccccgaaa agtgccacct gcagc
//
