LOCUS       sequence-345756-        1736 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Donor vector for endogenous tagging of human UBQLN2 at the 
            C-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBQLN2_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             446..1333
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
ORIGIN
        1 tgtaggccca gtcaccccca taggccccat aggccctata gtccctttta cccccatagg
       61 ccccattggg cccataggac ccactggccc tgcagccccc cctggctcca ccggctctgg
      121 tggccccacg gggcctactg tgtccagcgc tgcacctagt gaaaccacga gtcctacatc
      181 agaatctgga cccaaccagc agttcattca gcaaatggtg caggccctgg ctggagcaaa
      241 tgctccacag ctgccgaatc cagaagtcag atttcagcaa caactggaac agctcaacgc
      301 aatggggttc ttaaaccgtg aagcaaactt gcaggcccta atagcaacag gaggcgacat
      361 caatgcagcc attgaaaggc tgctgggctc ccagccatcg ggtggaggag gttctggagg
      421 cggtggaagt ggtggcggag gtagcgccga gatcggcacc ggctttccct tcgatcctca
      481 ctacgtggaa gtgctgggcg agagaatgca ctatgtggac gtgggcccca gagatggaac
      541 ccctgtgctg tttctgcacg gcaaccctac cagcagctac gtgtggcgga acatcatccc
      601 tcacgtggcc cctacacaca gatgtatcgc ccctgacctg atcggcatgg gcaagagcga
      661 taagcccgac ctgggctact tcttcgacga ccacgtgcgg ttcatggacg cctttattga
      721 ggccctcggc ctggaagagg tggtgctggt tattcacgat tggggcagcg ccctgggatt
      781 ccactgggcc aagagaaacc ccgagagagt gaagggaatc gccttcatgg aattcatcag
      841 gcccattcct acctgggacg agtggcctga gttcgccaga gagacattcc aggccttcag
      901 aaccaccgac gtgggcagaa agctgatcat cgaccagaac gtgttcatcg agggcaccct
      961 gcctatggga gtcgtcagac cactgaccga ggtggaaatg gaccactaca gagagccctt
     1021 tctgaacccc gtggaccggg aacctctttg gagattcccc aacgagctgc ccattgccgg
     1081 cgagcctgct aatattgtgg ccctggtgga agagtacatg gactggctgc atcagagccc
     1141 cgtgcctaag ctgctgtttt ggggaacacc cggcgtgctg attcctcctg ctgaagctgc
     1201 cagactggcc aagagcctgc ctaattgcaa ggccgtggat atcggccctg gcctgaatct
     1261 gctgcaagag gacaaccccg atctgatcgg ctccgagatt gccagatggc tgagcaccct
     1321 ggaaatcagc ggataatcac atttctgtac ctggaaaaaa aatgtatctt atttttgata
     1381 atggctctta aatctttaaa cacacacaca aaatcgttct ttactttcat tttgattctt
     1441 ttaaatctgt ctagttgtaa gtctaatatg atgcatttta agatggagtc cctccctcct
     1501 acttccctca ctccctttct cctttgctta tttttcctac cttcccttcc tcttgtctcc
     1561 ccactccctc cctctttgtt tccttccttc cttatttcct ttagtttcct tccttagccg
     1621 ttttgagtgg tgggaatcaa tgctgtttca ctcaaaagtg ttgcatgcaa acacttctct
     1681 ttattctgca tttattgtga tttttggaaa caggtatcaa ccttcacagt tgggtg
//
