LOCUS       sequence-345805-        1736 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Donor vector for endogenous tagging of human SERPINI1 at the 
            C-terminus with halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1736)
  TITLE     iNDI donor vectors for endogenous tagging with Halotag
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SERPINI1_Halo_C_allele
FEATURES             Location/Qualifiers
     source          1..1736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             446..1333
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
ORIGIN
        1 ctgttatcct agctactaga ggtgttgggg gtggaaggaa tgcttgagct caggagttca
       61 cagttacagt aagctctgat agagccactg cactccagcc tgggcatcag agcaagagcc
      121 tttctctaga aggaaagaag aaaggaaagg aaagggaaag ggaaaaggaa ggaagagaga
      181 gaggaaggaa ggaaggacag gaggaaggaa ggaaggaagg aaggaaggag aaaataacat
      241 attttcatta attgtaagca agaaggaaga taaatatttt attattttgt tcaccccctc
      301 ttttgtttac ttctgaacca atacaggtac aattctattc atgggacgag tcatgcatcc
      361 tgaaacaatg aacacaagtg gacatgattt cgaagaactt ggtggaggag gttctggagg
      421 cggtggaagt ggtggcggag gtagcgccga gatcggcacc ggctttccct tcgatcctca
      481 ctacgtggaa gtgctgggcg agagaatgca ctatgtggac gtgggcccca gagatggaac
      541 ccctgtgctg tttctgcacg gcaaccctac cagcagctac gtgtggcgga acatcatccc
      601 tcacgtggcc cctacacaca gatgtatcgc ccctgacctg atcggcatgg gcaagagcga
      661 taagcccgac ctgggctact tcttcgacga ccacgtgcgg ttcatggacg cctttattga
      721 ggccctcggc ctggaagagg tggtgctggt tattcacgat tggggcagcg ccctgggatt
      781 ccactgggcc aagagaaacc ccgagagagt gaagggaatc gccttcatgg aattcatcag
      841 gcccattcct acctgggacg agtggcctga gttcgccaga gagacattcc aggccttcag
      901 aaccaccgac gtgggcagaa agctgatcat cgaccagaac gtgttcatcg agggcaccct
      961 gcctatggga gtcgtcagac cactgaccga ggtggaaatg gaccactaca gagagccctt
     1021 tctgaacccc gtggaccggg aacctctttg gagattcccc aacgagctgc ccattgccgg
     1081 cgagcctgct aatattgtgg ccctggtgga agagtacatg gactggctgc atcagagccc
     1141 cgtgcctaag ctgctgtttt ggggaacacc cggcgtgctg attcctcctg ctgaagctgc
     1201 cagactggcc aagagcctgc ctaattgcaa ggccgtggat atcggccctg gcctgaatct
     1261 gctgcaagag gacaaccccg atctgatcgg ctccgagatt gccagatggc tgagcaccct
     1321 ggaaatcagc ggataagtta ctttatttga ataacaagga aaacagtaac taagcacatt
     1381 atgtttgcaa ctcgtatata tttaggattt gtgttttaca gtatatctta agataatatt
     1441 taaaatagtt ccagataaaa acaatatatg taaattataa gtaacttgtc aaggaatgtt
     1501 atcagtatta agctaatggt cctgttatgt cattgtgttt gtgtgctgtt gtttaaaata
     1561 aaagtaccta ttgaacatgt gactagatct atttttttat tttagaagtt gtagtataaa
     1621 gatatctcta ggtgaacttt ggaaacaatg tttctctacc tcttaaatct cttatgcagg
     1681 atcaaaacat catagtaaaa tagcattata aacttatgca taacaagcaa taacaa
//
