LOCUS       sequence-345852-        7929 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Constitutive overexpression of the 3'UTR of the murine Arid3a gene 
            after a GFP, to evaluate miRNA binding. Regions not containing 
            miR-125b or let-7c binding regions not included..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.EFS.eGFP.mArid3a-3'UTR-WT
FEATURES             Location/Qualifiers
     source          1..7929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     157..178
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    454..1042
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(507..527)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(925..936)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1114..1347
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1425..1546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1462..1481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1516..1535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1586..1721
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1741..1760)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1742..1760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1770..1787)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1770..1786)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1779..1801)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1928..2383
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2015..2034)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2225..2246
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2409..2513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2514..2582,2583..3374)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2732..2751)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3545..4133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4034..4053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4287..4304
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4421..4442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4457..4462,4463..4480,4481..4487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4495..4511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4500..4522
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4519..4535
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4519..4535
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4554..4574
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4556..4574
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        4619..4998
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        5055..5246
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5078..5098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5265..5491
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5492..5672
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5719..5844
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6337..6570
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6755..6799
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7012..7129
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7199..7410
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      7427..7436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7433..7435,7436..7438,7439..7929,1..223)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7433..7435,7436..7438,7439..7929,1..220)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7478..7499)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7739..7758)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
       61 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
      121 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      181 cgccgccggg atcactctcg gcatggacga gctgtacaag taaacatgtc gcacgtctgt
      241 ttacctcatg aatcccggca catatttgtg ttcattcagg gaaacagact ttttttaggg
      301 taactggctc agggatatag cagccagcgc aagtttcttt ctacctcagg tcttttgaca
      361 cctggtacct cagggcttgg cgttgagaca cccaaactca gggcaaagaa ctgttttcta
      421 cctctggtca ggcgtaaaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
      481 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
      541 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
      601 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
      661 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
      721 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
      781 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
      841 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
      901 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
      961 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     1021 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     1081 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     1141 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     1201 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     1261 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     1321 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     1381 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     1441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     1561 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     1621 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     1681 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     1741 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     1801 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1861 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1921 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1981 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2041 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2101 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2161 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2221 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2281 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2341 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     2401 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2461 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2521 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2581 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2641 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2701 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2761 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2821 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2881 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2941 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3001 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3061 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3121 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3181 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3241 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3301 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3361 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3421 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3481 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3541 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3601 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3661 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3721 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3781 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3841 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3901 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3961 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4021 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4081 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4141 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4201 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4261 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4321 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4381 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     4441 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4501 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     4561 accctcacta aagggaacaa aagctggagc tgcaagcggt accgtcgacc tgcaggttga
     4621 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     4681 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4741 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4801 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4861 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4921 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     4981 gtcatcgcta ttaccatggc gcgccatgca gggcctgaaa taacctctga aagaggaact
     5041 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     5101 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5161 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5221 ctcaattagt cagcaaccat agtcccttaa gaggcgcgcc ttaatgtagt cttatgcaat
     5281 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     5341 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     5401 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     5461 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     5521 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5581 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5641 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     5701 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     5761 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     5821 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     5881 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     5941 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6001 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     6061 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     6121 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     6181 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     6241 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     6301 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     6361 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     6421 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     6481 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     6541 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     6601 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     6661 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     6721 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     6781 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     6841 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     6901 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     6961 cccgagggga caattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     7021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     7081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     7141 cagggacagc agagatccac tttggcgccg gctcgagttg gctccggtgc ccgtcagtgg
     7201 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
     7261 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     7321 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     7381 tttcgcaacg ggtttgccgc cagaacacag gtgtcgtgac ggatccgcca ccatggtgag
     7441 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     7501 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     7561 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     7621 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     7681 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7741 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7801 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7861 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7921 ggtgaactt
//
