LOCUS       sequence-346683-        6788 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Myc Mutant (delta Myc BoxII) tagged with His and V5 in 
            Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Tu WB, Shiah YJ, Lourenco C, Mullen PJ, Dingar D, Redel C, Tamachi 
            A, Ba-Alawi W, Aman A, Al-Awar R, Cescon DW, Haibe-Kains B, 
            Arrowsmith CH, Raught B, Boutros PC, Penn LZ
  TITLE     MYC Interacts with the G9a Histone Methyltransferase to Drive 
            Transcriptional Repression and Tumorigenesis.
  JOURNAL   Cancer Cell. 2018 Oct 8;34(4):579-595.e8. doi: 
            10.1016/j.ccell.2018.09.001.
  PUBMED    30300580
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.09"; journalName: "Cancer Cell"; date: 
            "2018-10-8- 8"; volume: "34"; issue: "4"; pages: "579-595.e8"
COMMENT     Sequence Label: pcDNA3.1 His-V5 Myc dMBII
FEATURES             Location/Qualifiers
     source          1..6788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(68..88)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        73..402
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      253..388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     315..334
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             469..1263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(523..542)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1133..1152
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1437..1558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1474..1493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1528..1547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1607..1623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1607..1623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1620..1642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1631..1647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1655..1661,1662..1679,1680..1685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1700..1721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1838..1855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2009..2597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2089..2108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2768..3559,3560..3628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3391..3410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3629..3733)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3808..3827)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3999..4378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4379..4582
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4533..4553
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4627..4646
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4627..4645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5589..5615
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5859..5888
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5973..6014
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6024..6041
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(6105..6123)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6106..6123)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6143..6160)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6149..6373
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(6419..6788,1..59)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6506..6525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6716..6737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
       61 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      241 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      301 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
      361 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
      421 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
      481 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      541 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      601 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      661 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      721 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      781 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      841 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      901 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      961 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     1021 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1081 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1141 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1201 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1261 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     1321 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     1381 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     1441 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1561 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     1621 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1681 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1741 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1801 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1861 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1921 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2401 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2461 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2521 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2581 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2641 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2701 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2761 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2821 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2881 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2941 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3001 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3061 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3121 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3181 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3241 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3301 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3361 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3421 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3481 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3541 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3601 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3661 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3721 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     3781 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3841 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     3901 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     3961 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     4021 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4081 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4141 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4201 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4261 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4321 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4381 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4441 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4501 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     4561 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     4621 cgaaattaat acgactcact atagggagac ccaagcttgg taccgagctc ggatccacca
     4681 tgcccctcaa cgttagcttc accaacagga actatgacct cgactacgac tcggtgcagc
     4741 cgtatttcta ctgcgacgag gaggagaact tctaccagca gcagcagcag agcgagctgc
     4801 agcccccggc gcccagcgag gatatctgga agaaattcga gctgctgccc accccgcccc
     4861 tgtcccctag ccgccgctcc gggctctgct cgccctccta cgttgcggtc acacccttct
     4921 cccttcgggg agacaacgac ggcggtggcg ggagcttctc cacggccgac cagctggaga
     4981 tggtgaccga gctgctggga ggagacatgg tgaaccagag tttcatctgc gacccggacg
     5041 acgagacctt catcaaaaac ctcgtctcag agaagctggc ctcctaccag gctgcgcgca
     5101 aagacagcgg cagcccgaac cccgcccgcg gccacagcgt ctgctccacc tccagcttgt
     5161 acctgcagga tctgagcgcc gccgcctcag agtgcatcga cccctcggtg gtcttcccct
     5221 accctctcaa cgacagcagc tcgcccaagt cctgcgcctc gcaagactcc agcgccttct
     5281 ctccgtcctc ggattctctg ctctcctcga cggagtcctc cccgcagggc agccccgagc
     5341 ccctggtgct ccatgaggag acaccgccca ccaccagcag cgactctgag gaggaacaag
     5401 aagatgagga agaaatcgat gttgtttctg tggaaaagag gcaggctcct ggcaaaaggt
     5461 cagagtctgg atcaccttct gctggaggcc acagcaaacc tcctcacagc ccactggtcc
     5521 tcaagaggtg ccacgtctcc acacatcagc acaactacgc agcgcctccc tccactcgga
     5581 aggactatcc tgctgccaag agggtcaagt tggacagtgt cagagtcctg agacagatca
     5641 gcaacaaccg aaaatgcacc agccccaggt cctcggacac cgaggagaat gtcaagaggc
     5701 gaacacacaa cgtcttggag cgccagagga ggaacgagct aaaacggagc ttttttgccc
     5761 tgcgtgacca gatcccggag ttggaaaaca atgaaaaggc ccccaaggta gttatcctta
     5821 aaaaagccac agcatacatc ctgtccgtcc aagcagagga gcaaaagctc atttctgaag
     5881 aggacttgtt gcggaaacga cgagaacagt tgaaacacaa acttgaacag ctacggaact
     5941 cttgtgcggg tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     6001 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagaattc tgcagatatc
     6061 catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta
     6121 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6181 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6241 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6301 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6361 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6781 gggatttt
//
