LOCUS       sequence-346731-       10927 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR
            activation domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Camara E, Lenitz I, Nygard Y
  TITLE     A CRISPR activation and interference toolkit for industrial 
            Saccharomyces cerevisiae strain KE6-12.
  JOURNAL   Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.
  PUBMED    32884066
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-3"; volume: "10(1):14605. doi"; pages: " 
            10.1038/s41598-020-71648-w"
COMMENT     Sequence Label: EC2_4_dCas9_VPR_sgRNA
FEATURES             Location/Qualifiers
     source          1..10927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        322..735
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             758..778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4886..4906
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4913..5062
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5087..5107
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5537..5893
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5912..6481
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     misc_RNA        7043..7118
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     gene            7285..8640
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     CDS             7629..8438
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      8443..8640
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    8645..9148
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(9192..10007)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9192..10007)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9999..10018
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10232..10820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10721..10740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaattc gcatctagac tgaactggcc gataattgca gacgaacgcc ttgccaacag
       61 ggagttcttc agagacatgg aggctcaaaa cgaaattatt gacagcctag acatcaatag
      121 tcatacaaca gaaagcgacc acccaacttt ggctgataat agcgtataaa caatgcatac
      181 tttgtacgtt caaaatacaa tgcagtagat atatttatgc atattacata taatacatat
      241 cacataggaa gcaacaggcg cgttggactt ttaattttcg aggaccgcga atccttacat
      301 cacacccaat cccccacaag tgatccccca cacaccatag cttcaaaatg tttctactcc
      361 ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc
      421 aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac
      481 taaaggtttg gaaaagaaaa aagacaccgc ctcgtttctt tttcttcgtc gaaaaaggca
      541 ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt tttttctctt
      601 tcgatgacct cccattgata tttaagttaa taaacggtca tcaatttctc aagtttcagt
      661 ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa agaaagcata
      721 gcaatctaat ctaagtttta attacaaaag atctatgcca aagaagaaga gaaaggtagg
      781 cagcgacaag aagtattcta tcggactggc catcgggact aatagcgtcg ggtgggccgt
      841 gatcactgac gagtacaagg tgccctctaa gaagttcaag gtgctcggga acaccgaccg
      901 gcattccatc aagaaaaatc tgatcggagc tctcctcttt gattcagggg aaaccgctga
      961 agcaacccgc ctcaagcgga ctgctagacg gcggtacacc aggaggaaga accggatttg
     1021 ttaccttcaa gagatattct ccaacgaaat ggcaaaggtc gacgacagct tcttccatag
     1081 gctggaagaa tcattcctcg tggaagagga taagaagcat gaacggcatc ccatcttcgg
     1141 taatatcgtc gacgaggtgg cctatcacga gaaataccca accatctacc atcttcgcaa
     1201 aaagctggtg gactcaaccg acaaggcaga cctccggctt atctacctgg ccctggccca
     1261 catgatcaag ttcagaggcc acttcctgat cgagggcgac ctcaatcctg acaatagcga
     1321 tgtggataaa ctgttcatcc agctggtgca gacttacaac cagctctttg aagagaaccc
     1381 catcaatgca agcggagtcg atgccaaggc cattctgtca gcccggctgt caaagagccg
     1441 cagacttgag aatcttatcg ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa
     1501 cctgattgct ctttcacttg ggctgactcc caatttcaag tctaatttcg acctggcaga
     1561 ggatgccaag ctgcaactgt ccaaggacac ctatgatgac gatctcgaca acctcctggc
     1621 ccagatcggt gaccaatacg ccgacctttt ccttgctgct aagaatcttt ctgacgccat
     1681 cctgctgtct gacattctcc gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc
     1741 aatgattaag cggtatgatg agcaccacca ggacctgacc ctgcttaagg cactcgtccg
     1801 gcagcagctt ccggagaagt acaaggaaat cttctttgac cagtcaaaga atggatacgc
     1861 cggctacatc gacggaggtg cctcccaaga ggaattttat aagtttatca aacctatcct
     1921 tgagaagatg gacggcaccg aagagctcct cgtgaaactg aatcgggagg atctgctgcg
     1981 gaagcagcgc actttcgaca atgggagcat tccccaccag atccatcttg gggagcttca
     2041 cgccatcctt cggcgccaag aggacttcta cccctttctt aaggacaaca gggagaagat
     2101 tgagaaaatt ctcactttcc gcatccccta ctacgtggga cccctcgcca gaggaaatag
     2161 ccggtttgct tggatgacca gaaagtcaga agaaactatc actccctgga acttcgaaga
     2221 ggtggtggac aagggagcca gcgctcagtc attcatcgaa cggatgacta acttcgataa
     2281 gaacctcccc aatgagaagg tcctgccgaa acattccctg ctctacgagt actttaccgt
     2341 gtacaacgag ctgaccaagg tgaaatatgt caccgaaggg atgaggaagc ccgcattcct
     2401 gtcaggcgaa caaaagaagg caattgtgga ccttctgttc aagaccaata gaaaggtgac
     2461 cgtgaagcag ctgaaggagg actatttcaa gaaaattgaa tgcttcgact ctgtggagat
     2521 tagcggggtc gaagatcggt tcaacgcaag cctgggtacc taccatgatc tgcttaagat
     2581 catcaaggac aaggattttc tggacaatga ggagaaagag gacatccttg aggacattgt
     2641 cctgactctc actctgttcg aggaccggga aatgatcgag gagaggctta agacctacgc
     2701 ccatctgttc gacgataaag tgatgaagca acttaaacgg agaagatata ccggatgggg
     2761 acgccttagc cgcaaactca tcaacggaat ccgggacaaa cagagcggaa agaccattct
     2821 tgatttcctt aagagcgacg gattcgctaa tcgcaacttc atgcaactta tccatgatga
     2881 ttccctgacc tttaaggagg acatccagaa ggcccaagtg tctggacaag gtgactcact
     2941 gcacgagcat atcgcaaatc tggctggttc acccgctatt aagaagggta ttctccagac
     3001 cgtgaaagtc gtggacgagc tggtcaaggt gatgggtcgc cataaaccag agaacattgt
     3061 catcgagatg gccagggaaa accagactac ccagaaggga cagaagaaca gcagggagcg
     3121 gatgaaaaga attgaggaag ggattaagga gctcgggtca cagatcctta aagagcaccc
     3181 ggtggaaaac acccagcttc agaatgagaa gctctatctg tactaccttc aaaatggacg
     3241 cgatatgtat gtggaccaag agcttgatat caacaggctc tcagactacg acgtggacgc
     3301 catcgtccct cagagcttcc tcaaagacga ctcaattgac aataaggtgc tgactcgctc
     3361 agacaagaac cggggaaagt cagataacgt gccctcagag gaagtcgtga aaaagatgaa
     3421 gaactattgg cgccagcttc tgaacgcaaa gctgatcact cagcggaagt tcgacaatct
     3481 cactaaggct gagaggggcg gactgagcga actggacaaa gcaggattca ttaaacggca
     3541 acttgtggag actcggcaga ttactaaaca tgtagcccaa atccttgact cacgcatgaa
     3601 taccaagtac gacgaaaacg acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc
     3661 caagctggtc agcgatttca gaaaggactt tcaattctac aaagtgcggg agatcaataa
     3721 ctatcatcat gctcatgacg catatctgaa tgccgtggtg ggaaccgccc tgatcaagaa
     3781 gtacccaaag ctggaaagcg agttcgtgta cggagactac aaggtctacg acgtgcgcaa
     3841 gatgattgcc aaatctgagc aggagatcgg aaaggccacc gcaaagtact tcttctacag
     3901 caacatcatg aatttcttca agaccgaaat cacccttgca aacggtgaga tccggaagag
     3961 gccgctcatc gagactaatg gggagactgg cgaaatcgtg tgggacaagg gcagagattt
     4021 cgctaccgtg cgcaaagtgc tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt
     4081 gcaaaccgga ggcttttcta aggaatcaat cctccccaag cgcaactccg acaagctcat
     4141 tgcaaggaag aaggattggg accctaagaa gtacggcgga ttcgattcac caactgtggc
     4201 ttattctgtc ctggtcgtgg ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt
     4261 gaaggaactg ctgggtatca ccattatgga gcgcagctcc ttcgagaaga acccaattga
     4321 ctttctcgaa gccaaaggtt acaaggaagt caagaaggac cttatcatca agctcccaaa
     4381 gtatagcctg ttcgaactgg agaatgggcg gaagcggatg ctcgcctccg ctggcgaact
     4441 tcagaagggt aatgagctgg ctctcccctc caagtacgtg aatttcctct accttgcaag
     4501 ccattacgag aagctgaagg ggagccccga ggacaacgag caaaagcaac tgtttgtgga
     4561 gcagcataag cattatctgg acgagatcat tgagcagatt tccgagtttt ctaaacgcgt
     4621 cattctcgct gatgccaacc tcgataaagt ccttagcgca tacaataagc acagagacaa
     4681 accaattcgg gagcaggctg agaatatcat ccacctgttc accctcacca atcttggtgc
     4741 ccctgccgca ttcaagtact tcgacaccac catcgaccgg aaacgctata cctccaccaa
     4801 agaagtgctg gacgccaccc tcatccacca gagcatcacc ggactttacg aaactcggat
     4861 tgacctctca cagctcggag gggatcccaa gaaaaagcgc aaggtaggat ccgacgcatt
     4921 ggacgatttt gatctggata tgctgggaag tgacgccctc gatgattttg accttgacat
     4981 gcttggttcg gatgcccttg atgactttga cctcgacatg ctcggcagtg acgcccttga
     5041 tgatttcgac ctggacatgc tgattaactc tagaagttcc ggatctccga aaaagaaacg
     5101 caaagttggt agccagtacc tgcccgacac cgacgaccgg caccggatcg aggaaaagcg
     5161 gaagcggacc tacgagacat tcaagagcat catgaagaag tcccccttca gcggccccac
     5221 cgaccctaga cctccaccta gaagaatcgc cgtgcccagc agatccagcg ccagcgtgcc
     5281 aaaacctgcc ccccagcctt accccttcac cagcagcctg agcaccatca actacgacga
     5341 gttccctacc atggtgttcc ccagcggcca gatctctcag gcctctgctc tggctccagc
     5401 ccctcctcag gtgctgcctc aggctcctgc tcctgcacca gctccagcca tggtgtctgc
     5461 actggctcag gcaccagcac ccgtgcctgt gctggctcct ggacctccac aggctgtggc
     5521 tccaccagcc cctaaaccta cacaggccgg cgagggcaca ctgtctgaag ctctgctgca
     5581 gctgcagttc gacgacgagg atctgggagc cctgctggga aacagcaccg atcctgccgt
     5641 gttcaccgac ctggccagcg tggacaacag cgagttccag cagctgctga accagggcat
     5701 ccctgtggcc cctcacacca ccgagcccat gctgatggaa taccccgagg ccatcacccg
     5761 gctcgtgaca ggcgctcaga ggcctcctga tccagctcct gcccctctgg gagcaccagg
     5821 cctgcctaat ggactgctgt ctggcgacga ggacttcagc tctatcgccg acatggactt
     5881 ctccgcactg ctgggtagcg gatcgggatc tcgggattcc agggaaggga tgtttttgcc
     5941 gaagcctgag gccggctccg ctattagtga cgtgtttgag ggccgcgagg tgtgccagcc
     6001 aaaacgaatc cggccatttc atcctccagg aagtccatgg gccaaccgcc cactccccgc
     6061 cagcctcgca ccaacaccaa ccggtccagt acatgagcca gtcgggtcac tgaccccggc
     6121 accagtccct cagccactgg atccagcgcc cgcagtgact cccgaggcca gtcacctgtt
     6181 ggaggatccc gatgaagaaa cgagccaggc tgtcaaagcc cttcgggaga tggccgatac
     6241 tgtgattccc cagaaggaag aggctgcaat ctgtggccaa atggaccttt cccatccgcc
     6301 cccaaggggc catctggatg agctgacaac cacacttgag tccatgaccg aggatctgaa
     6361 cctggactca cccctgaccc cggaattgaa cgagattctg gataccttcc tgaacgacga
     6421 gtgcctcttg catgccatgc atatcagcac aggactgtcc atcttcgaca catctctgtt
     6481 ttaactcgag tggcataaag caatcttgat gaggataatg attttttttt gaatatacat
     6541 aaatactacc gtttttctgc tagattttgt gatgacgtaa ataagtacat attacttttt
     6601 aagccaagac aagattaagc attaacttta cccttttctt tctaagtttc aatattagtt
     6661 atcactgttt aaaagttatg gcgagaacgt cggcggttaa aatatattac cctgaacggc
     6721 tgccaaacca gatgtcaaca cagctacaac gatgtgcttc agtattacat tttttgcctt
     6781 caacgccttg attgttctat ttttgctaat aataaatcta tttcatcgga ctaaaagtcc
     6841 attagttgta agcggattta gctcagttgg gagagcgcca gactgaagaa aaacttcggt
     6901 caagtcatct ggaggtcctg tgttcgatcc acagaattcg cagatggccg gcatggtccc
     6961 agcctcctcg ctggcgccgg ctgggcaaca ccttcgggtg gcgaatggga ctcgctatcg
     7021 tcttctcacg aagacttact cagttttaga gctagaaata gcaagttaaa ataaggctag
     7081 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttatttt ttgtcactat
     7141 tgttatgtaa aatgccacct ctgacagtat ggaacgcaaa cttctgtcta gtggatagct
     7201 gagcagttac agagatgtta cgaaccacta gtgcactgca gtacagttta gcttgcctcg
     7261 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     7321 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     7381 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     7441 ggctcctcgc tccagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     7501 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     7561 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     7621 aaacaaaaat gggtaaggaa aagactcacg tttcgaggcc gcgattaaat tccaacatgg
     7681 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     7741 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     7801 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     7861 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     7921 cgatccccgg caaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     7981 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     8041 cttttaacag cgatcgcgta tttcgtctgg ctcaggcgca atcacgaatg aataacggtt
     8101 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     8161 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     8221 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     8281 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     8341 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     8401 aattgcagtt tcatttgatg ctcgatgagt ttttctaaag taactgacaa taaaaagatt
     8461 cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa
     8521 tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag
     8581 ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg
     8641 gagtatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt
     8701 aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta
     8761 aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag
     8821 gtattaatgc cgaattgttt catcttgtct gtgtagaaaa ccacacacga aaatcctgtg
     8881 attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct
     8941 aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt
     9001 ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa
     9061 acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga
     9121 ggcgcgtgta agttacaggc aagcgatccg tcccgagcgg ccgcgtgtta caaccaatta
     9181 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     9241 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     9301 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     9361 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     9421 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     9481 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     9541 attcgtgatt gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa
     9601 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     9661 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     9721 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     9781 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     9841 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     9901 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     9961 tttaatcgcg gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta
    10021 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
    10081 atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct
    10141 gagttgaagg atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg
    10921 cggccgc
//
