LOCUS       sequence-346990-        8778 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Poorly reversible opto-kinesin for optogenetic heterodimerization to
            iLID via SSPB(micro).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Nijenhuis W, van Grinsven MMP, Kapitein LC
  TITLE     An optimized toolbox for the optogenetic control of intracellular 
            transport.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 
            10.1083/jcb.201907149.
  PUBMED    32328628
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133834. doi"; 
            pages: " 10.1083/jcb.201907149"
COMMENT     Sequence Label: pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     941..963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1026..1035
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2008..2017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2631..2633,2634..3344)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2631..2633,2634..3344)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2673..2694)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2934..2953)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3281..3302
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(3474..3680,3681..3683,3684..3803)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    3936..4070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3973..3992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4027..4046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4084..4100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4084..4100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4097..4119)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4108..4124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4132..4138,4139..4156,4157..4162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4177..4198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4315..4332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4486..5074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4566..4585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5245..6036,6037..6105))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5868..5887
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6106..6210)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6278..6296
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6334..6356)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6456..6475
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6669..6691
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6683..6700
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6684..6700
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8501..8776
                     /label=chicken beta-actin promoter
ORIGIN
        1 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
       61 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      121 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      181 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      241 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      301 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
      361 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
      421 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
      481 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
      541 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
      601 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
      661 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
      721 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
      781 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
      841 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgtccg
      901 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
      961 accggcgggg tttatatctt cccttctctg ttcctccgca gcccccaagc taggcaattg
     1021 ggcgcgccac catggccggg gcttcggtga aggtggcggt gcgtgtccgc cccttcaact
     1081 cccgggaaat gagccgagac tccaagtgca tcattcagat gtctggaagc accaccacta
     1141 ttgtcaaccc caaacagccc aaggagacac ccaagagctt cagctttgat tactcctact
     1201 ggtcacatac ctcgccggag gacatcaact atgcctctca gaagcaggtc tacagggaca
     1261 ttggggaaga gatgctacag catgcctttg agggctacaa tgtgtgcatc tttgcctatg
     1321 ggcagacggg ggccggcaag tcctacacca tgatggggaa acaggagaag gaccagcaag
     1381 gcatcattcc acagctctgc gaagacctct tctctcgaat caacgacacc accaacgaca
     1441 acatgtccta ctctgtggag gtcagctaca tggagattta ctgtgagcga gtccgtgacc
     1501 tcctgaaccc caagaacaaa ggcaacctgc gtgtgaggga gcaccctctg ctggggcctt
     1561 atgtggagga cctctccaag ctggctgtaa cctcctacaa tgacatccag gacctcatgg
     1621 actcagggaa caaggccagg actgtggcgg ccacaaacat gaatgagacc agcagccgtt
     1681 ctcacgcagt cttcaacatc atctttaccc agaagcgcca tgatgcagag accaacatca
     1741 ccacagagaa agtgagcaaa atcagcctgg tggacctggc aggcagtgaa cgagccgact
     1801 ccacaggagc caagggcacg cgactcaagg agggggccaa catcaacaag tctttgacca
     1861 ccctgggaaa ggtcatctct gctctggctg aaatggactc tggacccaac aagaacaaga
     1921 aaaagaagaa gacagatttc atcccttacc gagactccgt attgacatgg cttctccggg
     1981 aaaacctcgg tggcaactca aggacagcca tggtggcagc cctgagccct gcggacatca
     2041 actatgatga gaccctcagc acactgagat atgcagatcg ggccaaacaa atccgctgca
     2101 atgccatcat caatgaagac cccaactcta gaggtggcgg gggcagtggg ggatcccata
     2161 ctctttatgc ccccggtgga tatgacatta tgggatatct gtgccagatc atgaaccggc
     2221 caaacccgca ggtggaactg ggccccgtgg atacatcctg cgccttgatt ctttgtgacc
     2281 tgaaacagaa agacaccccg atagtttacg cgagtgaagc cttcctctac atgacaggtt
     2341 acagcaacgc agaggtgctg ggccggaatt gccggtttct gcaaagccct gacggcatgg
     2401 tgaagcccaa gagcacccgg aagtacgtgg atagtaacac aatcaatact atgcgcaagg
     2461 caatcgacag gaatgccgag gtgcaggttg aagtagtcaa ttttaaaaag aatggacagc
     2521 gatttgttaa tttcctgact atgatacctg ttagggacga aacaggcgag tatcgatact
     2581 ctatgggatt ccagtgcgaa gtcgacggga gcgggggtgg gagtaagctt gtgagcaagg
     2641 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2701 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2761 tgaagttgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     2821 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     2881 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2941 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3001 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3061 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcaa
     3121 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     3181 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     3241 agtccaaact gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3301 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggctagc ggaggcggtg
     3361 gctcaggcgg aggagggtca ggagggggag gcagtggcgg gggcggctct ggaggagggg
     3421 gttctggtgg tggtagctac gtagatctga gcggcctgac cctgcaggaa ttcagctccc
     3481 cgaaacgccc taagctgctg cgtgaatatt acgattggct ggttgataac agctttaccc
     3541 catatctggt ggtggatgcc acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag
     3601 acggtcagat cgtgctgaat ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg
     3661 attttatcca gttcaacgcc cagtttaagg gcgtgtctcg tgaactgtat atcccgatgg
     3721 gtgccgctct ggccatttac gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag
     3781 aaatctatga cgagctgaat attggttaaa ccggtcctag ataagtaatg atcataatca
     3841 gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     3901 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     3961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aattcgtaat
     4081 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4921 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5161 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5221 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5341 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5521 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6241 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     6301 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     6361 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     6421 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     6481 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6541 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6601 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     6661 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagttgg gatctttgca
     6721 ttggcccacg gctctcagga tggggatgct ccccttcagc acccggttcc ccttggaaac
     6781 tgatggtcct ggctctgtgg catggcagtg gcactgtgag gagcccccta ccagcagcac
     6841 acagtggggt tggcactgcc acgctccgga tgccgcgctc tgatccaacc ccataatcaa
     6901 gggaacccga attgccccat cattgccccc accaccccca tcctgccggg ccctcacacc
     6961 ccacgctgcc ttctggtgac attccccagc ccaaacccac ggcttcatgg ctaccgcggg
     7021 gcatttccca ttgccgcccc attatcagct ctgcacacct cccgctgtac ccatgcctcg
     7081 tggctgccct tctttgacgt ataatcttct aattaatacc cggccttgtc aaagtggagc
     7141 acaaacgtta attaattccc cagcaggcag gtaattaaca gtgtgactcc ctttttgctg
     7201 cgagtggggc tgatacagag agatgtggca ctatggagcc cacggggtcc tggcactggg
     7261 tgcccacgga ggtccccatg tgctgcagtg tcaccgcctc cgaggagaca gtattgtccc
     7321 tgcggtgtcc ctgcagctca gctctgtcca cagggccacc tccagtttgg aggggacaca
     7381 atgcagcccc gatgcaaccc atcctcgcag catcccaggg acaaagaccc cactgcaaga
     7441 ccgcacacag ggctgggtcc cgctccccta atatctacag tgcttcttgc atggcccctt
     7501 aatcaatgca gttaatcagc atgcgctcat gcaccgctct ggagctgcaa agcccctcgc
     7561 agcgctgctc accaacaccg cgcaccgccc cggcccagcc tgcagcacgc gctgcaaaca
     7621 ggaaagaaac aaaatattgc ccaaatgtag gcaaaggcat tcggctgcct tgacctccgc
     7681 cgggccgggc cctgcctgac tcagctcctt actcagcgct cgcttcctcc ctccggctgc
     7741 caccgccgca gcgcacaccc tgacaaagag tggcccttaa cgggctctga ggtgcaccca
     7801 gcagtgcact cagcagtcca agggccggcc tggaggtttg caccgctacg tgctgacatt
     7861 agcattgaac ttggccctgg gtagtgctgc aggccgggcg gggtgggtgt agagagtgca
     7921 gcgcgcgttg cacccggtgc cccttcccct cccttgcatc ccagcaggct gcaccccagc
     7981 accaggcccg tgcatgcatg ctcctggtgt tattgcagcc tggtgcatgc atgcgtcttg
     8041 atggtgcagc gctgtgcatg catcctcctt ggtgtgtagc agtctagtgc atgcataccc
     8101 ctcggtgtta ttgctgctct gtgcacgcac gctcattgta tcacttcatc ccagtgcatg
     8161 cactcacact ggagcgattg ctgctcggtg cacgcacact cattgtatca ctgcagctca
     8221 gtggctgcac gcacaccggt gttattgctg ctcggtgcgt gcatgcacat cagtgtcgct
     8281 gcagctcagt gcatgcacgc tcattgccca tcgctatccc tgcctctcct gctggcgctc
     8341 cccgggaggt gacttcaagg ggaccgcagg accacctcgg gggtgggggg agggctgcac
     8401 acgcggaccc cgctccccct ccccaacaaa gcactgtgga atcaaaaagg ggggaggggg
     8461 gatggagggg cgcgtcacac ccccgcccca caccctcacc tcgaggtgag ccccacgttc
     8521 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     8581 taattatttt gtgcagcgat gggggcgggg gggggggggg cgcgcgccag gcggggcggg
     8641 gsggggsgrg gggsggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     8701 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     8761 aagcgcgcgg cgggcggg
//
