LOCUS       sequence-347045-       12384 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Doxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with 
            dTomato as reporter fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 12384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: 
            SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
FEATURES             Location/Qualifiers
     source          1..12384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    235..256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(271..276,277..294,295..301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    309..325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     314..336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     333..349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     368..388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        370..388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        433..812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        869..1060
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(892..912)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1079..1305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1306..1486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1533..1658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2151..2384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2569..2613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2826..2943
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2989..3367
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3141..3159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3177..3195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3213..3231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3276..3300
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5184..5249
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(5262..5836,5837..5839,5840..5848)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5429..5446)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5656..5675
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5855..6556
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5988..6006)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6256..6275
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6450..6473
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        6581..7080
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6616..6639)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7013..7032
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      7087..7096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7093..7095,7096..7098,7099..7809)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             7822..7878
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7879..8625
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8652..9240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8705..8725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9123..9134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9312..9545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9623..9744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9660..9679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9714..9733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9784..9919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9939..9958)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9940..9958)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9968..9985)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9968..9984)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9977..9999)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10126..10581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10213..10232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10423..10444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10607..10711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10712..10780,10781..11572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10930..10949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11743..12331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12232..12251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
       61 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      181 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      241 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      301 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      361 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cggtaccgtc
      421 gacctgcagg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      781 acatctacgt attagtcatc gctattacca tggcgcgcca tgcagggcct gaaataacct
      841 ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt
      901 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      961 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     1021 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg
     1081 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     1141 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     1201 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     1261 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     1321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2761 acccacctcc caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc
     2821 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2881 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2941 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gtttactccc
     3001 tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac
     3061 cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag
     3121 agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca gtttactccc
     3181 tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag aacgtataag
     3241 ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa ccgtcagatc
     3301 gcctggagca attccacaac acttttgtct tataccaact ttccgtacca cttcctaccc
     3361 tcgtaaagac cggtgccacc atgaagttgc aagccgtcat ggaaactctc attcagcggc
     3421 aacagagagc acgacaagaa ctggaagctc gccaagcccc tcctcctccc cctcctgaac
     3481 ctacaggagt tcgcgctcga acaaccatga ctgacgagga cagagagcca gaaaatgccc
     3541 ggatgcatcg gactcagatg gcagccttgg ccgccatgcg cgctgctgct gctggattgg
     3601 gtcacccttc tagtcccgga ggctctgaag acgggccccc aatctccggt gatgaagaca
     3661 ccgccaggga gggtactctt tctagtccag cactccatgg cagcgtgttg gagggtgctg
     3721 gtcatgccga gggagatcgg catttgatgg atgttgggtc agacgatgat gatacaaagt
     3781 ctaaatggga agagcaggag ttggaagaat tgggagagga ggaagaggaa gaagaggagg
     3841 aggacgattt tgaggaggaa gaagaagagg aggagggttt gggtcctccc gaatccgcct
     3901 ccctggggac agctggactc ttcacccgaa aggcccctcc agcacaggca tttcggggtg
     3961 acggagggcc ccgcatgctc tctggacccg aaagattggg tcctggcccc gcccacccaa
     4021 gtcacatggc ctcacagatg cctccacccg accatggcga ctggactttc gaggaacaat
     4081 ttaaacagct ttatgagctc gacgcagatc caaaacggaa agagtttctg gatgatctct
     4141 tctcctttat gcagaagcga ggcacccctg taaacaggat tccaataatg gctaagcaag
     4201 tactggacct gtttatgctg tatgtccttg taactgagaa gggagggctc gtcgaagtaa
     4261 tcaacaagaa actttggaga gaaattacaa agggattgaa tttgcctacc agtataacct
     4321 cagctgcatt cacactgaga actcagtaca tgaaatatct ctatccctat gaatgcgaac
     4381 gcaggggtct ttcttctcct aatgaactcc aagctgctat tgatagtaat aggagagaag
     4441 gcaggcgcca gagcttcggg ggaagcctct tcgcttatag ccccagtgga gcccattcaa
     4501 tgctccctag tccaaaactc cctgtcactc cactcggact ggctgcttct actaatggct
     4561 catcaattac cccagcacct aaaatcaaga aggaagaaga ctcagcaatc ccaattaccg
     4621 tgcccggtag gttgcctgtg tccttggccg gccatcctgt tgtggccgca caagcagcag
     4681 cagtacaggc cgctgccgca caggccgctg tcgctgccca agcagctgca ctcgaacaac
     4741 ttcgagaaaa actggaatcc accgagccac ctgagaagaa aatggctctg gttgcagatg
     4801 agcaacagag acttatgcag cgcgctgttc agcagtcctt tctggcaatg accgcccaac
     4861 ttcccatgaa cattagaatt aatagccaag cttccgaaag ccgccaagat agtgccgttt
     4921 ccttgacatc tgcaaatggt tccaatagta tttcaatgag tgttgaaatg aatgggatag
     4981 tatatactgg cgtccttttt gcccagcctc ctcctcctac agcaccttca gcaccaggta
     5041 agggcggagt cagctctatt ggtacaaata ccactaccgg aagtcgcact ggggccagtg
     5101 ggtcaaccgt ctctggcggg caggtgggac ttcctggcgt ttctactccc accatgagtt
     5161 ccaccagtaa caattccctg ccagactaca aggaccacga cggtgactac aaggaccacg
     5221 acatcgacta caaggacgac gacgacaagt aaacgcgttc gcccctctcc ctcccccccc
     5281 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     5341 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     5401 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     5461 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     5521 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     5581 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     5641 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5701 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt
     5761 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5821 aaaacacgat gataatatgg ccacaaccgg atccatggtg agcaagggcg aggaggtcat
     5881 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
     5941 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6001 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
     6061 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
     6121 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
     6181 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
     6241 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     6301 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     6361 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     6421 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     6481 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat
     6541 ggacgagctg tacaagtgag cgatatcgcg taattctacc gggtagggga ggcgcttttc
     6601 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     6661 ggcctctggc ctcgcacaca ttccacatcc accggaaggc gccaaccggc tccgttcttt
     6721 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     6781 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtgtcactag tctcgtgcag
     6841 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     6901 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     6961 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcca ccggaggccc ggcattctgc
     7021 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     7081 gctagcgcca ccatgggatc taagggcgag gaactgttca ccggcgtggt gcccatcctg
     7141 gtggaactgg atggcgacgt gaacggccac aagttctctg tgcggggaga gggcgaaggc
     7201 gacgccacaa atggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     7261 ccttggccta ccctcgtgac cacactgacc tacggcgtgc agtgcttcag cagatacccc
     7321 gaccatatga agcggcacga cttcttcaag agcgccatgc ccgagggcta cgtgcaggaa
     7381 cggaccatca gcttcaagga cgacggcacc tacaagacca gagccgaagt gaagttcgag
     7441 ggcgacaccc tcgtgaaccg gatcgagctg aagggcatcg atttcaaaga ggacggcaac
     7501 atcctgggcc acaagctgga gtacaacttc aacagccaca atgtgtatat caccgccgac
     7561 aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacgtgga agatggcagc
     7621 gtgcagctgg ccgaccacta ccagcagaac acccccatcg gagatggccc cgtgctgctg
     7681 cccgacaacc actacctgag cacccagagc gtgctgagca aggaccccaa cgagaagcgg
     7741 gaccacatgg tgctgctgga attcgtgacc gccgctggca tcacccacgg catggacgag
     7801 ctgtataagg gaggaagcgg cgccaccaat ttcagcctgc tgaaacaggc cggcgacgtg
     7861 gaagagaacc ctggccctat gagccggctg gacaagagca aagtgatcaa cagcgccctg
     7921 gaactgctga acggcgtggg catcgagggc ctgaccaccc ggaagctggc ccagaaactg
     7981 ggcgtggaac agcccaccct gtactggcac gtgaagaaca agcgggccct gctggacgcc
     8041 ctgcccatcg agatgctgga ccggcaccac acccacagct gccctctgga aggcgagagc
     8101 tggcaggact tcctgcggaa caacgccaag agctacagat gcgccctgct gagccaccgg
     8161 gacggcgcca aagtgcacct gggcaccaga cccaccgaga agcagtacga gacactggaa
     8221 aaccagctgg ccttcctgtg ccagcagggc ttcagcctgg aaaacgccct gtacgccctg
     8281 agcgccgtgg gccacttcac cctgggctgc gtgctggaag aacaggaaca ccaggtcgcc
     8341 aaagaggaaa gagagacacc caccaccgac agcatgcccc ccctgctgaa gcaggccatc
     8401 gagctgttcg acagacaggg cgccgagccc gccttcctgt tcggcctgga actgatcatc
     8461 tgcggcctcg agaagcagct gaagtgcgag agcggcggac ccaccgacgc cctggacgac
     8521 ttcgacctgg acatgctgcc cgccgatgcc ctggatgatt ttgatctgga tatgctccct
     8581 gctgacgctc tcgatgactt cgatctcgat atgctgccag gctaatgtac aagtgatcgc
     8641 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     8701 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     8761 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     8821 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     8881 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     8941 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     9001 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     9061 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     9121 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     9181 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     9241 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9301 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     9361 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9421 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9481 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9541 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9601 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     9661 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9721 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     9781 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9901 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     9961 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
    10021 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    10081 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
    10141 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    10201 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    10261 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10321 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    10381 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10441 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    10501 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    10561 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10621 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10681 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10741 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10801 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10861 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10921 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    10981 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    11041 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    11101 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    11161 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    11221 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    11281 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
    11341 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    11401 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    11461 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    11521 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    11581 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11641 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11701 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11761 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11821 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    11881 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    11941 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    12001 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    12061 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    12121 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    12181 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    12241 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    12301 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    12361 cctggccttt tgctggcctt ttgc
//
