LOCUS       sequence-347572-        9171 bp    DNA     circular SYN 14-APR-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses EGFP under the control of a 
            PGK promoter. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest eGFP
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1748..1872
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1897..1902,1903..1920,1921..1927)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1981..2640
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2047..2066)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2982..3287
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3166..3185
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3328..3452)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    3483..4071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3536..3556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3954..3965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4085..4584
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4120..4143)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4517..4536
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4622..4631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4628..4630,4631..4633,4634..5347)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4628..4630,4631..4633,4634..5344)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4673..4694)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4934..4953)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5281..5302
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5463..5696
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5768..5889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5805..5824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5859..5878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5929..6064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6084..6103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6085..6103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6113..6130)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6113..6129)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6122..6144)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6271..6726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6358..6377)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6568..6589
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6752..6856
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6857..6925,6926..7717)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7075..7094)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7888..8476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8377..8396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8630..8647
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8764..8785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8800..8805,8806..8823,8824..8830)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8838..8854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8843..8865
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8862..8878
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8862..8878
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8897..8917
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8899..8917
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8945..9171
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgtccag tgtggtggaa ttgagtattc cagtgtggtg gaattctgca
     1741 gatatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     1801 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     1861 ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1921 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
     1981 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     2041 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     2101 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     2161 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     2221 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     2281 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     2341 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     2401 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     2461 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     2521 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     2581 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     2641 agatctggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     2701 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
     2761 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     2821 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     2881 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     2941 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
     3001 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     3061 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     3121 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     3181 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     3241 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     3301 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
     3361 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     3421 gtttctcgtt cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     3481 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3841 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     4081 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     4141 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     4201 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     4261 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     4321 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     4381 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     4441 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     4501 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     4561 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccacactc ctgcaggtaa
     4621 agccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4681 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     4741 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     4801 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     4861 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     4921 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     4981 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5041 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5101 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5161 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5221 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5281 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5341 caagtaaagc ggccgcgctc tagagccgac gcgtatgcat ctcgagggcc cggtaccttt
     5401 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5461 actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5521 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5581 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5641 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5701 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5761 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5881 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     5941 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6001 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6061 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6241 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6661 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6721 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7081 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7141 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7201 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7261 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7321 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7381 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7441 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7501 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7561 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7621 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7681 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7921 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7981 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8041 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8101 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8161 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8221 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8281 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8341 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8401 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8461 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8521 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8581 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8641 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8701 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8761 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8821 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8881 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8941 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     9001 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     9061 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     9121 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa c
//
