LOCUS       sequence-348301-        9871 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3159
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2625..2649)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2657..2698
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2809..3397
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2862..2882)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3280..3291)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3469..3702
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3780..3901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3817..3836)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3871..3890
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3941..4076
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4096..4115)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4097..4115)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4125..4142)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4125..4141)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4134..4156)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4283..4738
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4370..4389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4580..4601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4764..4868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4869..4937,4938..5729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5087..5106)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5900..6488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6389..6408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6642..6659
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6776..6797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6812..6817,6818..6835,6836..6842)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6850..6866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6855..6877
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6874..6890
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6874..6890
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6909..6929
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6911..6929
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6957..7183
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7184..7364
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7411..7536
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8029..8262
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8447..8491
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8722..8932
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8744..8940
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8791..8926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8972..9574
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8972..8991)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9468..9488
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9617..9734
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtcgtc catcttgcca ttcacgccgc cagttgtgaa
     1261 gagactgctg ggatggaaga agtcagctgg tgggtctgga ggagcaggcg gaggagagca
     1321 gaatgggcag gaagaaaagt ggtgtgagaa agcagtgaaa agtctggtga agaagctaaa
     1381 gaaaacagga cgattagatg agcttgagaa agccatcacc actcaaaact gtaatactaa
     1441 atgtgttacc ataccaagca cttgctctga aatttgggga ctgagtacac caaatacgat
     1501 agatcagtgg gatacaacag gcctttacag cttctctgaa caaaccaggt ctcttgatgg
     1561 tcgtctccag gtatcccatc gaaaaggatt gccacatgtt atatattgcc gattatggcg
     1621 ctggcctgat cttcacagtc atcatgaact caaggcaatt gaaaactgcg aatatgcttt
     1681 taatcttaaa aaggatgaag tatgtgtaaa cccttaccac tatcagagag ttgagacacc
     1741 agttttgcct ccagtattag tgccccgaca caccgagatc ctaacagaac ttccgcctct
     1801 ggatgactat actcactcca ttccagaaaa cactaacttc ccagcaggaa ttgagccaca
     1861 gagtaattat attccagaaa cgccacctcc tggatatatc agtgaagatg gagaaacaag
     1921 tgaccaacag ttgaatcaaa gtatggacac aggctctcca gcagaactat ctcctactac
     1981 tctttcccct gttaatcata gcttggattt acagccagtt acttactcag aacctgcatt
     2041 ttggtgttcg atagcatatt atgaattaaa tcagagggtt ggagaaacct tccatgcatc
     2101 acagccctca ctcactgtag atggctttac agacccatca aattcagaga ggttctgctt
     2161 aggtttactc tccaatgtta accgaaatgc cacggtagaa atgacaagaa ggcatatagg
     2221 aagaggagtg cgcttatact acataggtgg ggaagttttt gctgagtgcc taagtgatag
     2281 tgcaatcttt gtgcagagcc ccaattgtaa tcagagatat ggctggcacc ctgcaacagt
     2341 gtgtaaaatt ccaccaggct gtaatctgaa gatcttcaac aaccaggaat ttgctgctct
     2401 tctggctcag tctgttaatc agggttttga agccgtctat cagctaacta gaatgtgcac
     2461 cataagaatg agttttgtga aagggtgggg agcagaatac cgaaggcaga cggtaacaag
     2521 tactccttgc tggattgaac ttcatctgaa tggacctcta cagtggttgg acaaagtatt
     2581 aactcagatg ggatcccctt cagtgcgttg ctcaagcatg tcatacccaa ctttcttgta
     2641 caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta
     2701 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     2761 aaaggacgat ttcggtcgaa catagacgac cggacgcgtt aagtcgacaa tcaacctctg
     2821 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2881 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2941 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3001 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3061 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3121 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3181 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3241 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3301 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3361 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     3421 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3481 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     3541 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     3601 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     3661 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     3721 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     3781 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3841 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3901 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     3961 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4021 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4081 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4141 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4201 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4261 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4321 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4381 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4441 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4501 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4561 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4621 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4681 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4741 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4801 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4861 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4921 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4981 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5041 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5101 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5161 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5221 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5281 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5341 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5401 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5461 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5521 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5581 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5641 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5701 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5761 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6601 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6661 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6721 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6781 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6841 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6901 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     6961 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7021 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     7081 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     7141 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     7201 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7261 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7321 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7381 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7441 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7501 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7561 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7621 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7681 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7741 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7801 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7861 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     7921 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     7981 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8041 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8101 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8161 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8221 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8281 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8341 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8401 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8461 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8521 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     8581 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     8641 ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt
     8701 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca
     8761 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8821 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8881 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8941 aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     9001 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     9061 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     9121 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     9181 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     9241 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9301 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9361 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9421 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9481 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     9541 ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg
     9601 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     9661 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     9721 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
     9781 aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
     9841 gaccttctag gtcttgaaag gagtgggaat t
//
