LOCUS       sequence-348316-        4808 bp    DNA     circular SYN 15-APR-2025
DEFINITION  High constitutive expression of Firefly Luciferase in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
  TITLE     Direct delivery and fast-treated Agrobacterium co-culture 
            (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
  JOURNAL   Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9.
  PUBMED    36253612
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 
            10.1038/s41596-022-00749-9."
COMMENT     Sequence Label: pMOD_A5801
FEATURES             Location/Qualifiers
     source          1..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1366..1711
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1654..1674
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1729..3381
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1795..1813)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      3391..3639
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        3716..3820
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccag atttgccttt tcaatttcag
      901 aaagaatgct aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat
      961 ctacccgagc aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt
     1021 caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag
     1081 aagtactatt ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga
     1141 gattggagtc tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca
     1201 aatagaggac ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt
     1261 acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta
     1321 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
     1381 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
     1441 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
     1501 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     1561 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat
     1621 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     1681 ataaggaagt tcatttcatt tggagagaac acgggggact cctgcaggat ggaagacgcc
     1741 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     1801 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     1861 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     1921 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     1981 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     2041 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     2101 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     2161 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     2221 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     2281 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     2341 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     2401 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     2461 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     2521 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     2581 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     2641 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     2701 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     2761 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     2821 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     2881 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     2941 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     3001 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     3061 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     3121 ttcgacgctg gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     3181 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     3241 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     3301 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     3361 ggcggaaaga tcgccgtgtg acgtcgacga tatgaagatg aagatgaaat atttggtgtg
     3421 tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa
     3481 tttgtggctt tttctaatat taaatgaatg taagatcaca ttataatgaa taaacaaatg
     3541 tttctataat ccattgtgaa tgttttgttg gatctcttct gcagcatata actactgtat
     3601 gtgctatggt atggactatg gaatatgatt aaagataagc cagagctctg gtgacggacg
     3661 tgggcaggtg tgatgaaaag ccttagggtc aggtggcact tttcggggaa atgtgcgatg
     3721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctaa tgagacaata
     3781 accctgataa atgcttcaat tatattgaaa aaggaagagt atgagtatcc aacatttccg
     3841 tgtcgccctt attccctttt ttgcggcctt ctgccttcct gtttttgctc acccagaaac
     3901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc cgagtgggtt acattgaact
     3961 ggatctcaac agcggcaaaa tcttagagag ttttcgcccc gaagaacgct tcccaatgat
     4021 gagcactttc aaagttctgc tatgtggcgc ggtgttatcc cgtattgatg ctgggcaaga
     4081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt caccagtcac
     4141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgtagtgctg ccataaccat
     4201 gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga aggagctaac
     4261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4381 tctccgcaaa ctattaactg gcgaactact tactcttgct tcacgccagc aactcattga
     4441 ctggatggag gcggacaaag ttgcaggacc acttctgcgc tcggcacttc cggctggctg
     4501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4681 actgtcagac gaagggaaat aaatagtagc ccgcctgatg tgcgggcttt tttttggtgg
     4741 tggttaccag tgtcgcttag ctaccgtagg tcctcttagg cggtccgatt agcgacatag
     4801 tcatcaat
//
