LOCUS       sequence-348484-       10975 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3165
FEATURES             Location/Qualifiers
     source          1..10975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3729..3753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3761..3802
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3913..4501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3966..3986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4384..4395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4573..4806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4884..5005
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4921..4940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4975..4994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5045..5180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5200..5219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5201..5219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5229..5246)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5229..5245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5238..5260)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5387..5842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5474..5493)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5684..5705
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5868..5972
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5973..6041,6042..6833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6191..6210)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7004..7592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7493..7512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7746..7763
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7880..7901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7916..7921,7922..7939,7940..7946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7954..7970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7959..7981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7978..7994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7978..7994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8013..8033
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8015..8033
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8061..8287
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8288..8468
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8515..8640
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9133..9366
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9551..9595
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9826..10036
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9848..10044
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9895..10030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10076..10678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10076..10095)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10572..10592
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10721..10838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcggc ctccgcggcg gcagcctcgg cagcagcggc
     1261 ctcggccgcc tctggcagcc cgggcccggg cgagggctcc gctggcggcg aaaagcgctc
     1321 caccgcccct tcggccgcag cctcggcctc tgcctcagcc gcggcgtcgt cgcccgcggg
     1381 gggcggcgcc gaggcgctgg agctgctgga gcactgcggc gtgtgcagag agcgcctgcg
     1441 acccgagagg gagccccgcc tgctgccctg tttgcactcg gcctgtagtg cctgcttagg
     1501 gcccgcggcc cccgccgccg ccaacagctc gggggacggc ggggcggcgg gcgacggcac
     1561 cgtggtggac tgtcccgtgt gcaagcaaca gtgcttctcc aaagacatcg tggagaatta
     1621 tttcatgcgt gatagtggca gcaaggctgc caccgacgcc caggatgcga accagtgctg
     1681 cactagctgt gaggataatg ccccagccac cagctactgt gtggagtgct cggagcctct
     1741 gtgtgagacc tgtgtagagg cgcaccagcg ggtgaagtac accaaggacc atactgtgcg
     1801 ctctactggg ccagccaagt ctcgggatgg tgaacgtact gtctattgca acgtacacaa
     1861 gcatgaaccc cttgtgctgt tttgtgagag ctgtgatact ctcacctgcc gagactgcca
     1921 gctcaatgcc cacaaggacc accagtacca gttcttagag gatgcagtga ggaaccagcg
     1981 caagctcctg gcctcactgg tgaagcgcct tggggacaaa catgcaacat tgcagaagag
     2041 caccaaggag gttcgcagct caatccgcca ggtgtctgac gtacagaagc gtgtgcaagt
     2101 ggatgtcaag atggccatcc tgcagatcat gaaggagctg aataagcggg gccgtgtgct
     2161 ggtcaatgat gcccagaagg tgactgaggg gcagcaggag cgcctggagc ggcagcactg
     2221 gaccatgacc aagatccaga agcaccagga gcacattctg cgctttgcct cttgggctct
     2281 ggagagtgac aacaacacag cccttttgct ttctaagaag ttgatctact tccagctgca
     2341 ccgggccctc aagatgattg tggatcccgt ggagccacat ggcgagatga agtttcagtg
     2401 ggacctcaat gcctggacca agagtgccga ggcctttggc aagattgtgg cagagcgtcc
     2461 tggcactaac tcaacaggcc ctgcacccat ggcccctcca agagccccag ggcccctgag
     2521 caagcagggc tctggcagca gccagcccat ggaggtgcag gaaggctatg gctttgggtc
     2581 aggagatgat ccctactcaa gtgcagagcc ccatgtgtca ggtgtgaaac ggtcccgctc
     2641 aggtgagggc gaggtgagcg gccttatgcg caaggtgcca cgagtgagcc ttgaacgcct
     2701 ggacctggac ctcacagctg acagccagcc acccgtcttc aaggtcttcc caggcagtac
     2761 cactgaggac tacaacctta ttgttattga acgtggcgct gccgctgcag ctaccggcca
     2821 gccagggact gcgcctgcag gaacccctgg tgccccaccc ctggctggca tggccattgt
     2881 caaggaggag gagacggagg ctgccattgg agcccctcct actgccactg agggccctga
     2941 gaccaaacct gtgcttatgg ctcttgcgga gggtcctggt gctgagggtc cccgcctggc
     3001 ctcacctagt ggcagcacca gctcagggct ggaggtggtg gctcctgagg gtacctcagc
     3061 cccaggtggt ggcccgggaa ccctggatga cagtgccacc atttgccgtg tctgccagaa
     3121 gccaggcgat ctggttatgt gcaaccagtg tgagttttgt ttccacctgg actgtcacct
     3181 gccggccctg caggatgtac caggggagga gtggagctgc tcactctgcc atgtgctccc
     3241 tgacctgaag gaggaggatg gcagcctcag cctggatggt gcagacagca ctggcgtggt
     3301 ggccaagctc tcaccagcca accagcggaa atgtgagcgt gtactgctgg ccctattctg
     3361 tcacgaaccc tgccgccccc tgcatcagct ggctaccgac tccaccttct ccctggacca
     3421 gcccggtggc accctggatc tgaccctgat ccgtgcccgc ctccaggaga agttgtcacc
     3481 tccctacagc tccccacagg agtttgccca ggatgtgggc cgcatgttca agcaattcaa
     3541 caagttaact gaggacaagg cagacgtgca gtccatcatc ggcctgcagc gcttcttcga
     3601 gacgcgcatg aacgaggcct tcggtgacac caagttctct gctgtgctgg tggagccccc
     3661 gccgatgagc ctgcctggtg ctggcctgag ttcccaggag ctgtctggtg gccctggtga
     3721 tggcccctgc ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc
     3781 tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat
     3841 ttcttggctt tatatatctt gtggaaagga cgacgaaatg cccatacagc tcccccgacg
     3901 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4321 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4441 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4501 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4561 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4621 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4681 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4741 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4801 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     4861 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     4921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4981 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     5041 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5101 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5161 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     5221 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     5281 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     5341 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     5401 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5461 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5521 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5581 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5641 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5701 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5761 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5821 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5881 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5941 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6001 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6061 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6121 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6181 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6241 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6301 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6361 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6421 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6481 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6541 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6601 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6661 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6721 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6781 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6841 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7141 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7381 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7681 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7741 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7801 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7861 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     7921 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7981 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     8041 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     8101 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     8161 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     8221 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     8281 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8341 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8401 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8461 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     8521 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8581 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8641 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8701 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8761 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8821 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8881 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8941 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     9001 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9061 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9121 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     9181 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9241 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9301 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9361 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9421 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9481 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9541 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9601 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9661 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9721 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     9781 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9841 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    10021 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
    10081 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
    10141 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
    10201 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
    10261 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
    10321 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    10381 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    10441 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    10501 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    10561 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10621 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
    10681 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10861 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
    10921 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
