LOCUS       sequence-348966-       10518 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the 
            puromycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Mustafa EH, Laven-Law G, Kikhtyak Z, Nguyen V, Ali S, Pace AA, Iggo 
            R, Kebede A, Noll B, Wang S, Winter JM, Dwyer AR, Tilley WD, Hickey 
            TE
  TITLE     Selective inhibition of CDK9 in triple negative breast cancer.
  JOURNAL   Oncogene. 2023 Nov 24. doi: 10.1038/s41388-023-02892-3.
  PUBMED    38001268
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2023 Nov 24. doi: 10.1038/s41388-023-02892-3."
COMMENT     Sequence Label: pDRM209 LTP (Luciferase tdTomato Puro)
FEATURES             Location/Qualifiers
     source          1..10518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..350
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    843..1076
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1261..1305
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1572..1689
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2291..2300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2297..3946
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2363..2381)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             3956..4015
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     regulatory      4028..4037
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4034..5461
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             5471..5524
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5525..6124
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5525..5544)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6021..6041
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6140..6728
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6193..6213)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6611..6622)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6815..7048
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7120..7241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7157..7176)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7211..7230
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7281..7416
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7436..7455)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7437..7455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7465..7482)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7465..7481)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7474..7496)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7623..8076
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7710..7729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7918..7939
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8102..8206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8207..8275,8276..9067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8425..8444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9238..9826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9727..9746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9980..9997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10114..10135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10147..10152,10153..10170,10171..10177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10185..10201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10190..10212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10209..10225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10209..10225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10244..10264
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10246..10264
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10292..10518
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcgactggtg agtacgccaa
      301 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      361 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      421 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      481 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      541 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      601 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      661 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      721 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      781 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      841 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
      901 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
      961 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1021 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1081 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1141 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1201 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1261 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1321 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1381 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1441 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     1501 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     1561 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1621 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1681 caaaatttta tcgatcacga gactagcctc gaggatatcg agatcgatta gtccaatttg
     1741 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     1801 agcctataga gtacgagcca tagatagaat aaaagatttt atttagtctc cagaaaaagg
     1861 ggggaatgaa agaccccacc tgtaggtttg gcaagctagg atcaaggtta ggaacagaga
     1921 gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1981 gccaagaaca gttggaacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2221 tcaataaaag agcccacaac ccctcactcg gcgcgatcga tgaattcgag ctcggtaccc
     2281 ggggatccaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
     2341 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
     2401 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga
     2461 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
     2521 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
     2581 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
     2641 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa
     2701 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa
     2761 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
     2821 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat
     2881 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
     2941 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
     3001 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
     3061 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
     3121 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
     3181 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     3241 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
     3301 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     3361 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     3421 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
     3481 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     3541 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
     3601 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
     3661 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
     3721 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     3781 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     3841 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     3901 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtggcta gcggacagtg
     3961 tactaattat gctctcttga aattggctgg agatgttgag agcaaccctg gacctcatat
     4021 accggtcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa
     4081 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4141 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     4201 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
     4261 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
     4321 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
     4381 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
     4441 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     4501 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
     4561 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt
     4621 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4681 gcgctccgag ggccgccacc acctgttcct ggggcatggc accggcagca ccggcagcgg
     4741 cagctccggc accgcctcct ccgaggacaa caacatggcc gtcatcaaag agttcatgcg
     4801 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     4861 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     4921 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     4981 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     5041 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     5101 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     5161 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     5221 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     5281 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     5341 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     5401 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
     5461 gggaagcgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
     5521 acctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     5581 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     5641 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5701 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5761 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5821 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5881 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5941 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6001 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6061 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6121 ctgactcgaa ctagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6181 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6241 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6301 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6361 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6421 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6481 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     6541 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6601 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6661 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6721 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     6781 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     6841 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6901 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6961 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7021 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7081 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7141 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7201 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7261 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7321 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7381 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     7441 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7501 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7561 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7621 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7681 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7741 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7801 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7861 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gaccacgttc tttaatagtg
     7921 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7981 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8041 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     8101 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     8161 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8221 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8281 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8341 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8401 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8461 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8521 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8581 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8641 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8701 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8761 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8821 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8881 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8941 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9001 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9061 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     9121 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9181 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9241 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9301 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9361 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9421 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9481 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9541 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9601 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9661 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9721 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9781 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9841 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9901 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    10021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10081 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    10141 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10201 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
    10261 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
    10321 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
    10381 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
    10441 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
    10501 ctagctcgat acataaac
//
