LOCUS       sequence-349065-        9874 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Template for in vitro transcription of PEmax.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen
            C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, 
            Liu DR
  TITLE     Enhanced prime editing systems by manipulating cellular determinants
            of editing outcomes.
  JOURNAL   Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 
            10.1016/j.cell.2021.09.018.
  PUBMED    34653350
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09"; journalName: "Cell"; date: "2021-10-28- 
            28"; volume: "184"; issue: "22"; pages: "5635-5652.e29"
COMMENT     Sequence Label: pT7-PEmax for IVT
FEATURES             Location/Qualifiers
     source          1..9874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             340..360
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             361..4461
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4519..4539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6640..6660
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6670..6696
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    6804..7028
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7099..7115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7099..7115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7112..7134)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7123..7139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7147..7153,7154..7171,7172..7177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7192..7213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7330..7347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7501..8089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7581..7600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8260..9051,9052..9120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8883..8902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9121..9225)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9304..9323)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9495..9874
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatctcgag ctcggtacct
      241 aatacgacac actataagga aataagagag aaaagaagag taagaagaaa tataagagcc
      301 accatgaaac ggacagccga cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc
      361 gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc
      421 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
      481 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
      541 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
      601 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
      661 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
      721 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
      781 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
      841 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
      901 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
      961 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagaaag
     1021 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     1081 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     1141 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     1201 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     1261 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     1321 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     1381 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     1441 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     1501 aagatggacg gcaccgagga actgctcgtg aagctgaaga gagaggacct gctgcggaag
     1561 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     1621 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     1681 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     1741 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     1801 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     1861 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     1921 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     1981 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     2041 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     2101 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     2161 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     2221 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     2281 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     2341 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     2401 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     2461 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     2521 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     2581 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     2641 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     2701 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     2761 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     2821 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc
     2881 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     2941 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     3001 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     3061 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     3121 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     3181 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     3241 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     3301 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     3361 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     3421 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     3481 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     3541 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     3601 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     3661 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     3721 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     3781 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     3841 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     3901 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     3961 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     4021 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     4081 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     4141 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     4201 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     4261 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     4321 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     4381 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     4441 ctgtctcagc tgggaggtga ctccggcgga agctctggtg gcagcaagcg gaccgccgac
     4501 ggctctgaat tcgagagccc taagaagaaa agaaaggtga gcggaggctc tagcggcgga
     4561 agcaccctga acattgaaga cgagtataga ctgcatgaaa caagcaagga acccgatgtg
     4621 tccctgggct ccacctggct gtccgacttt ccccaggcct gggccgagac aggaggaatg
     4681 ggcctggccg tgcggcaggc acccctgatc atccctctga aggccacctc tacacccgtg
     4741 agcatcaagc agtaccctat gtctcaggag gccagactgg gcatcaagcc tcacatccag
     4801 aggctgctgg accagggcat cctggtgcca tgccagagcc cctggaacac accactgctg
     4861 cccgtgaaga agccaggcac caatgactat agacccgtgc aggatctgag agaggtgaac
     4921 aagagggtgg aggatatcca ccccaccgtg cccaaccctt acaatctgct gtccggcctg
     4981 cccccttctc accagtggta tacagtgctg gacctgaagg atgccttctt ttgtctgaga
     5041 ctgcacccta ccagccagcc actgttcgcc tttgagtgga gggaccctga gatgggcatc
     5101 tctggccagc tgacctggac acgcctgcct cagggcttca agaatagccc aacactgttt
     5161 aacgaggccc tgcaccgcga cctggcagat ttccggatcc agcacccaga tctgatcctg
     5221 ctgcagtacg tggacgatct gctgctggcc gccaccagcg agctggattg ccagcaggga
     5281 acacgcgccc tgctgcagac cctgggaaac ctgggatata gggcatccgc caagaaggcc
     5341 cagatctgtc agaagcaggt gaagtacctg ggctatctgc tgaaggaggg ccagagatgg
     5401 ctgacagagg ccaggaagga gacagtgatg ggccagccaa cacccaagac cccaagacag
     5461 ctgagggagt tcctgggcaa agcaggattt tgcaggctgt tcatcccagg attcgcagag
     5521 atggcagcac ctctgtaccc actgaccaag ccgggcaccc tgtttaattg gggccctgac
     5581 cagcagaagg cctatcagga gatcaagcag gccctgctga cagcaccagc cctgggcctg
     5641 ccagacctga ccaagccttt cgagctgttt gtggatgaga agcagggcta cgccaagggc
     5701 gtgctgaccc agaagctggg accatggaga cggcccgtgg cctatctgtc caagaagctg
     5761 gacccagtgg cagcaggatg gccaccatgc ctgaggatgg tggcagcaat cgccgtgctg
     5821 acaaaggatg ccggcaagct gaccatggga cagccactgg tcatcctggc accacacgca
     5881 gtggaggccc tggtgaagca gcctccagat cgctggctgt ctaacgcccg gatgacacac
     5941 taccaggccc tgctgctgga caccgatcgc gtgcagtttg gccctgtggt ggccctgaat
     6001 ccagccaccc tgctgcctct gccagaggag ggcctgcagc acaactgtct ggacatcctg
     6061 gcagaggcac acggaacaag gccagacctg accgatcagc ccctgcctga cgccgatcac
     6121 acatggtata ccgatggaag ctccctgctg caggagggcc agaggaaggc aggagcagca
     6181 gtgaccacag agacagaagt gatctgggcc aaggccctgc cagcaggcac atccgcccag
     6241 cgggccgagc tgatcgccct gacccaggcc ctgaagatgg ccgagggcaa gaagctgaac
     6301 gtgtacacag actccagata tgccttcgcc accgcacaca tccacggaga gatctacagg
     6361 cgccggggct ggctgacctc tgagggcaag gagatcaaga acaaggatga gatcctggcc
     6421 ctgctgaagg ccctgtttct gcccaagcgg ctgagcatca tccactgtcc tggacaccag
     6481 aagggacact ccgccgaggc aaggggcaat cggatggccg accaggccgc cagaaaggct
     6541 gctattactg aaactcccga cacttccact ctgctgattg aaaactcctc cccttctggc
     6601 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagtctc ccaagaagaa gaggaaagtc
     6661 ggctctggcc ctgccgctaa gagagtgaag ctggactaat taattaagct gccttctgcg
     6721 gggcttgcct tctggccatg cccttcttct ctcccttgca cctgtacctc ttggtctttg
     6781 aataaagcct gagtaggaag cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6841 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6901 tgagaaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6961 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     7021 ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta
     7081 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag
     7201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8101 gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt
     8161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9241 agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtctactct
     9301 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     9361 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     9421 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     9481 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     9541 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     9601 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     9661 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     9721 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     9781 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     9841 gtacatctac gtattagtca tcgctattac catg
//
