LOCUS       sequence-34974-e         750 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Wei X, Prickett TD, Viloria CG, Molinolo A, Lin JC, Cardenas-Navia 
            I, Cruz P, Rosenberg SA, Davies MA, Gershenwald JE, Lopez-Otin C, 
            Samuels Y
  TITLE     Mutational and functional analysis reveals ADAMTS18 
            metalloproteinase as a novel driver in melanoma.
  JOURNAL   Mol Cancer Res. 2010 Nov . 8(11):1513-25.
  PUBMED    21047771
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2010 Nov . 8(11):1513-25."
COMMENT     Sequence Label: pCDF1-ADAMTS18 (G312E)
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..640)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(124..144)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     621..640
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 agtatcttta aattttgtaa tccagaggtt gattgtcgac tcaggcaccg ggcttgcggg
       61 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
      121 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
      181 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
      241 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
      301 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
      361 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
      421 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
      481 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccggatcg acggtgtggc
      541 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt
      601 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ctagcgtagg cgccggtcac
      661 agcttggatc tgtaacggcg cagaacagaa aacgaaacaa agacgtagag ttgagcaagc
      721 agggtcaggc aaagcgtgga gagccggctg
//
