LOCUS       sequence-350017-        9394 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3186
FEATURES             Location/Qualifiers
     source          1..9394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2148..2172)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2180..2221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2332..2920
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2385..2405)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2803..2814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2992..3225
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3303..3424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3340..3359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3394..3413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3464..3599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3619..3638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3620..3638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3648..3665)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3648..3664)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3657..3679)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3806..4261
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3893..3912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4103..4124
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4287..4391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4392..4460,4461..5252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4610..4629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5423..6011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5912..5931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6165..6182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6299..6320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6335..6340,6341..6358,6359..6365)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6373..6389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6378..6400
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6397..6413
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6397..6413
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6432..6452
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6434..6452
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6480..6706
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6707..6887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6934..7059
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7552..7785
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7970..8014
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8245..8455
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8267..8463
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8314..8449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8495..9097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8495..8514)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8991..9011
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9140..9257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggtttc agacgaagat gaattgaatc ttctggttat
     1261 tgtagttgat gccaacccaa tttggtgggg aaagcaagca ttaaaggaat ctcagttcac
     1321 tttatccaaa tgcatagatg ccgtgatggt gctgggaaat tcgcatttat tcatgaatcg
     1381 ttccaacaaa cttgctgtga tagcaagtca cattcaagaa agccgattct tatatcctgg
     1441 aaagaatggc agacttggag acttcttcgg agaccctggc aaccctcctg aatttaatcc
     1501 ctctgggagt aaagatggaa aatacgaact tttaacctca gcaaatgaag ttattgttga
     1561 agagattaaa gatctaatga ccaaaagtga cataaagggt caacatacag aaactttgct
     1621 ggcaggatcc ctggccaaag ccctttgcta cattcataga atgaacaagg aagttaaaga
     1681 caatcaggaa atgaaatcaa ggatattggt gattaaggct gcagaagaca gtgcgttgca
     1741 gtatatgaac ttcatgaatg tcatctttgc agcacagaaa cagaatattt tgattgatgc
     1801 ctgtgtttta gactccgact cagggctcct ccaacaggct tgtgacatca cgggaggact
     1861 gtacctgaag gtgcctcaga tgccttctct tctgcagtat ttgctgtggg tgtttcttcc
     1921 cgatcaagat cagagatctc agttaatcct cccacccccg gttcatgttg actacagggc
     1981 tgcttgcttc tgtcatcgaa atctcattga aattggttat gtctgttctg tgtgtttgtc
     2041 aatattctgc aatttcagcc ccatttgtac tacgtgcgag acagccttta aaatttctct
     2101 gcctccagtg ctgaaagcca agaaaaagaa actgaaagtg tctgcctgcc caactttctt
     2161 gtacaaagtg gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     2221 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2281 tggaaaggac gatagccact gagatggggt cgacacacgc gttaagtcga caatcaacct
     2341 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2401 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2461 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2521 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2581 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2641 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2701 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2761 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2821 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2881 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     2941 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3001 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3061 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3121 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3181 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     3241 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     3301 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3421 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     3481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3541 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3601 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     3661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3781 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4141 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4201 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4261 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6181 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6241 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6301 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     6361 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     6421 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     6481 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     6541 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     6601 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     6661 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     6721 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6781 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6841 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6901 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6961 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7021 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7081 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     7141 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     7201 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     7261 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     7321 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     7381 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     7441 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     7501 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     7561 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     7621 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     7681 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     7741 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     7801 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     7861 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     7921 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     7981 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8041 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     8101 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     8161 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     8221 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     8281 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8341 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8401 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     8461 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     8521 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     8581 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     8641 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     8701 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     8761 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     8821 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     8881 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8941 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9001 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9061 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
     9121 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9181 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9241 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
     9301 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     9361 atggaccttc taggtcttga aaggagtggg aatt
//
