LOCUS       sequence-350034-       10084 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3190
FEATURES             Location/Qualifiers
     source          1..10084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2838..2862)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2870..2911
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3022..3610
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3075..3095)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3493..3504)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3682..3915
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3993..4114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4030..4049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4084..4103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4154..4289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4309..4328)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4310..4328)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4338..4355)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4338..4354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4347..4369)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4496..4951
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4583..4602)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4793..4814
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4977..5081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5082..5150,5151..5942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5300..5319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6113..6701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6602..6621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6855..6872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6989..7010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7025..7030,7031..7048,7049..7055)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7063..7079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7068..7090
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7087..7103
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7087..7103
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7122..7142
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7124..7142
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7170..7396
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7397..7577
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7624..7749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8242..8475
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8660..8704
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8935..9145
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8957..9153
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9004..9139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9185..9787
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9185..9204)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9681..9701
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9830..9947
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggtcac ctgcgcccac ctgggccggc gcgcgcgact
     1261 cccggcagct cagccctctg cctgcccagg aacctgcttt tcccaagagg agagaatggc
     1321 tgctgggtac ctgccccgct ggtcccagga attagtaacc tttgaggacg tgtccatgga
     1381 cttctcccag gaggagtggg agttgctgga gcctgctcag aagaacctgt acagagaggt
     1441 gatgctggag aactacagga acgtggtctc cctggaagcc ttgaagaacc aatgtactga
     1501 tgtggggatt aaagagggtc cactttcccc agcacaaacc tcacaagtca ctagtctttc
     1561 ctcatggacg gggtatttac tttttcaacc agtggcttct tcccacttgg agcaaagaga
     1621 agccctgtgg atagaggaaa aaggaactcc tcaagcctcc tgttcagatt ggatgactgt
     1681 actaagaaac caagactcaa cttacaagaa ggtggctttg caggaggaac cagccagtgg
     1741 tataaatatg ataaagctta tcagagaaga tgggggatgg aagcagttag aggacagcca
     1801 tgaagacccc caggggcttt tgagccaaaa ggcatccctt cacgtagtgg ccgttcctca
     1861 ggagaaggct actgcatggc atggatttgg ggaaaatggt aatctgagcc cagcccttgt
     1921 tttatcacag ggaagctcta aagggaacca cttgtgtggc agcgagttag atattacaag
     1981 cttggcatcc gattcagtct taaaccacca tcagctggga tatgcagatc ggagaccttg
     2041 tgaaagtaat gaatgtggaa atgccatccg ccagaacagt cactttattc aacacggggg
     2101 gaagatgttt gtgtatttgg aaaatgggca gtcattgaac cacggtatgg ccctgactat
     2161 ccacaacaaa atcaacacgg cagagaaacc ctttgagtgc caccagtgtg ggaaggtgtt
     2221 caaccggagg cattctttga gcgaacatca aagaattcac acgggggaga aaccctacga
     2281 gtgtcaggag tgtgggcgag cctttacgca cagctccacc ctcacgcgcc atctgagaac
     2341 tcatactgga gagaagccct acgggtgcgg tgagtgcggg aaagccttca acaggatctc
     2401 atcgctgact cagcatcaga ggattcacac cggggaaaag ccctataaat gtgaagactg
     2461 tgggaaatcc ttctgccaga gctcttacct gatcttgcac aagaggacac acaccggaga
     2521 gaagccctac gaatgcagtg aatgtggaaa ggccttcagt gaccgttcct ctctcaacca
     2581 gcacgagcga actcacacgg gcgagaaccc ctatgaatgt aagcagtgtg ggagagcctt
     2641 cagccagagg tcttcccttg tgaggcacga gagaactcac actggagaga aaccctacag
     2701 gtgtcaggaa tgtgggaaag ccttcagcca gagctcatcc cttgtcacac atcagaaaac
     2761 tcacagcagc cagaaaacct ataaaatcat tgactgcggg aaagcgttct accagaacag
     2821 acatcttatt ggatattacc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     2881 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag
     2941 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaccgtcaca taacatgtga
     3001 ctcaatacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     3061 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3121 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3181 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3241 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3301 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3361 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3421 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3481 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3541 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3601 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3661 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3721 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3901 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     3961 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     4021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4141 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4261 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4321 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4381 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4441 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4501 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4561 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4621 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4681 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4741 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4801 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4861 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4921 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4981 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5041 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5101 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5161 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5221 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5281 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5341 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5401 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5461 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5521 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5581 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5641 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5701 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5761 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5821 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5881 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5941 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6001 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6061 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6121 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6181 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6241 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6301 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6361 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6421 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6481 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6541 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6661 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6721 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6781 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6841 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6901 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6961 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7021 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7081 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7141 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7201 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7261 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7321 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7381 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7441 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7501 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7561 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7621 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7681 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7741 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7801 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7861 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     7921 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7981 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8041 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8101 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8161 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8221 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8281 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8341 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8401 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8461 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8521 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8581 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8641 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8701 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8761 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8821 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     8881 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8941 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     9001 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9061 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9121 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9181 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9241 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9301 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9361 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9421 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9481 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9541 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9601 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9661 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9721 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9781 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9841 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9901 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9961 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
    10021 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
    10081 aatt
//
