LOCUS       sequence-35016-e         910 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: MKK7
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..724
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     682..704
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             752..772
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(899..910)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 attttgttta ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
      121 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
      181 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
      241 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
      301 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
      361 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
      421 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
      481 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
      541 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
      601 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
      661 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
      721 aaaatcggat gattacccag atctgggtac cgagaacctg tacttccaat ccaatgcggc
      781 gtcctccctg gagcagaagc tgtcccgcct ggaagccaag ctgaagcagg agaaccgtga
      841 ggcccgcagg aggatcgacc tcaacttgga tatcagccca cagcggccca ggcccaccct
      901 gcaactccca
//
