LOCUS       sequence-350385-       19814 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Destination Expression vector for monocot plant primer editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19814)
  AUTHORS   Wang L, Kaya HB, Zhang N, Rai R, Willmann MR, Carpenter SCD, Read 
            AC, Martin F, Fei Z, Leach JE, Martin GB, Bogdanove AJ
  TITLE     Spelling Changes and Fluorescent Tagging With Prime Editing Vectors 
            for Plants.
  JOURNAL   Front Genome Ed. 2021 Mar 4;3:617553. doi: 
            10.3389/fgeed.2021.617553. eCollection 2021.
  PUBMED    34713247
REFERENCE   2  (bases 1 to 19814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2021-03-4"; volume: "3:617553. doi"; pages: " 
            10.3389/fgeed.2021.617553. eCollection 2021"
COMMENT     Sequence Label: pPPEM
FEATURES             Location/Qualifiers
     source          1..19814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..61)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(45..61)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(58..80)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    69..85
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(93..99,100..117,118..123))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    138..159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        350..1026
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     963..983
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1093..2118
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2159..2333
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2411..2435
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2860..3654
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5079..5667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5568..5587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5821..5838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5853..5993
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5919..5938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6079..6101
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6337..6531
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6597..7670)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8099..8728)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10029..10053
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10241..10263
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10255..10272
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10256..10272
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10294..10418
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10443..10448,10449..10466,10467..10473)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             10829..11134
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11013..11032
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(11175..11299)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             13147..13212
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             13219..13239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(13264..15777,15778..15780,15781..17364)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             19540..19560
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      join(19586..19814,1..24)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cggtgtcatc tatgttacta gatcgggaat taattcgtaa tcatgtcata gctgtttcct
       61 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      241 agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg
      301 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
      361 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
      421 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
      481 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
      541 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
      601 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      661 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      721 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      781 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      841 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      901 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
      961 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
     1021 cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg cagatccggg
     1081 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     1141 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     1201 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     1261 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     1321 gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg ccgttcacag
     1381 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca accggtcgcg
     1441 gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     1501 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     1561 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     1621 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     1681 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     1741 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     1801 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     1861 gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     1921 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     1981 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     2041 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     2101 ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca agctcgagtt
     2161 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
     2221 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     2281 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa
     2341 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     2401 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     2461 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     2521 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     2581 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     2641 gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga
     2701 tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag
     2761 gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg
     2821 tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca
     2881 ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct
     2941 cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac
     3001 agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg
     3061 gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat
     3121 ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc
     3181 cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata
     3241 tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg
     3301 aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat
     3361 ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc
     3421 cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt
     3481 gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg
     3541 atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc
     3601 aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta
     3661 gaatgcatga ccaaaatccc ttaacgctga gagatcccct cataatttcc ccaaagcgta
     3721 accatgtgtg aataaatttt gagctagtag ggttgcagcc acgagtaagt cttcccttgt
     3781 tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg agagtacgtt
     3841 tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct gagcatgaac
     3901 gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga tatcaaaacg
     3961 ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac ttttcaagtc
     4021 tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt gtttgggtgt
     4081 tcgaatttca acaggtaagt tagttgctag aatccatggc tcctttgccg acgctgagta
     4141 gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt ttcggccttt
     4201 agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag tcagcctctt
     4261 atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga taggtttcca
     4321 gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta accagtacca
     4381 acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac tgacaatgag
     4441 cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt catgagcttt
     4501 ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag aacgaccgtg
     4561 tagtgcgact gaagctcgca ataccataag ccgtttttgc tcacggatat cagaccagtc
     4621 aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc aacggtatac
     4681 agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc gtttgatgtt
     4741 atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag tgagagtttt
     4801 acagtcaagt aaggcgtggc aagccaacgt taagctgttg agtcgtttta agtgtaattc
     4861 ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt tcgcacattt tgttgtctga
     4921 ttattgattt ttggcgaaac catttgatca tatgacaaga tgtgtatcta ccttaactta
     4981 atgattttga taaaaatcat taggggattc atcagccctt aacgtgagtt ttcgttccac
     5041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5221 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5821 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     5881 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     5941 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     6001 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6061 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6121 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     6181 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     6241 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     6301 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     6361 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     6421 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     6481 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
     6541 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     6601 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     6661 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
     6721 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
     6781 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
     6841 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
     6901 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
     6961 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
     7021 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
     7081 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
     7141 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
     7201 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
     7261 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
     7321 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
     7381 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
     7441 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
     7501 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
     7561 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
     7621 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
     7681 cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg
     7741 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
     7801 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
     7861 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
     7921 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
     7981 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
     8041 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
     8101 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
     8161 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
     8221 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
     8281 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
     8341 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
     8401 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
     8461 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
     8521 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
     8581 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
     8641 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
     8701 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
     8761 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
     8821 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
     8881 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
     8941 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
     9001 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
     9061 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
     9121 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
     9181 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
     9241 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
     9301 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
     9361 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
     9421 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
     9481 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
     9541 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
     9601 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
     9661 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
     9721 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
     9781 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
     9841 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
     9901 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
     9961 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
    10021 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
    10081 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac
    10141 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10201 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10261 acgacggcca gtgccatgct agagacgggg atcacaagtt tgtacaaaaa agctgaacga
    10321 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
    10381 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
    10441 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatcgatccg
    10501 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
    10561 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
    10621 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
    10681 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
    10741 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
    10801 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
    10861 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
    10921 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
    10981 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
    11041 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
    11101 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
    11161 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
    11221 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
    11281 ctttcttgta caaagtggtg aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc
    11341 cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca catatttttt
    11401 ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta aactttactc
    11461 tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat
    11521 gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt
    11581 tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata
    11641 cttcatccat tttattagta catccattta gggtttaggg ttaatggttt ttatagacta
    11701 atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc
    11761 tattttagtt tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa
    11821 aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga
    11881 gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca accagcgaac
    11941 cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg
    12001 gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat
    12061 tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg
    12121 caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc
    12181 cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc
    12241 acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg
    12301 ccgctcgtcc tccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
    12361 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
    12421 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
    12481 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
    12541 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
    12601 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
    12661 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
    12721 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
    12781 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
    12841 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
    12901 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
    12961 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
    13021 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
    13081 aggtcgactc tagaggatct ggaattcccg ggtaccggat ccgatccaaa tccgaatccg
    13141 gccatggact ataaggatca cgatggcgac tacaaggatc atgacattga ctataaggat
    13201 gacgacgata agatggcacc taagaagaaa aggaaagtcg gcattcatgg cgttccggca
    13261 gccgacaaaa agtatagcat cggcctcgat attgggacaa actctgtggg ctgggcggta
    13321 attaccgacg agtacaaggt gcctagtaag aaatttaaag tgctcggaaa cactgacagg
    13381 cactctataa agaagaacct gatcggggca ctgcttttcg actccggaga gacggcggag
    13441 gcgacgcgtc tcaagcgtac cgcgcgccgc aggtacacaa gaaggaagaa taggatctgc
    13501 tacttgcagg aaatcttcag taacgagatg gcgaaggtcg acgatagttt ctttcatcgg
    13561 ttggaagaat cgttcctcgt agaggaggac aaaaagcacg agcgtcaccc aatattcggg
    13621 aatattgttg acgaggttgc ctaccatgag aaatatccta caatatatca cctccgtaag
    13681 aagcttgtcg attcaactga taaggctgat ctcagactca tctatcttgc cctcgcacat
    13741 atgattaagt ttcgtggcca cttcttgatt gaaggcgacc tcaacccgga caactcagat
    13801 gttgacaagc tttttataca gctcgtccag acatataacc agctgtttga agagaatccc
    13861 atcaatgcga gtggggttga tgctaaggcc attttgtccg ccaggttgtc caaatctcgc
    13921 agactggaaa acctgatcgc acagcttccc ggtgaaaaga aaaacgggct cttcggcaat
    13981 ctcatcgcac tgtccctcgg cctcacccca aacttcaagt ctaacttcga cctggccgag
    14041 gatgcgaagc tccagctgtc aaaagataca tacgacgacg atttggacaa tctgcttgcg
    14101 caaataggcg accagtatgc ggacctgttc ctggctgcca aaaatctgtc agatgcaatc
    14161 ctcctgtccg atatattgcg tgtgaacacc gaaatcacga aggcaccgct tagcgcatcc
    14221 atgatcaaga gatacgacga gcaccatcag gacctcacac tcctcaaggc gcttgttcgt
    14281 cagcagcttc ccgagaaata taaggaaatt tttttcgatc aaagcaagaa tggatatgct
    14341 ggctatattg acggtggcgc ttcgcaggag gagttctata aattcattaa gccgattctg
    14401 gagaagatgg acggaacgga ggagctcctc gtcaagctta accgggaaga cctgttgcgg
    14461 aagcagagga cttttgataa cggctctatt ccgcaccaaa tccatctggg tgagttgcac
    14521 gcaatcttga gaagacaaga ggatttctac ccgttcctta aggataacag agagaagata
    14581 gaaaaaatac tgaccttcag gataccatac tatgtgggcc cactggcgcg cggaaatagt
    14641 cgtttcgcat ggatgactag aaagtccgaa gaaacgatca cgccatggaa ttttgaggaa
    14701 gtggtcgaca agggcgcctc tgcccagagc ttcatcgaaa ggatgaccaa ttttgacaaa
    14761 aatctgccta acgaaaaggt gcttccgaag cacagcctgt tgtatgaata cttcacagtt
    14821 tataacgagc tcactaaggt caagtacgtc acggagggca tgcgtaagcc tgctttcctg
    14881 tctggtgaac aaaaaaaggc gattgtggac ctccttttca agacgaaccg taaagttact
    14941 gtgaagcaac tgaaagagga ttactttaag aaaattgagt gcttcgacag tgtggagatt
    15001 tccggtgtcg aggaccggtt taacgccagc ctgggtacgt atcatgacct gcttaaaatt
    15061 atcaaggata aagatttcct ggataatgaa gagaacgaag atatactgga ggacattgtg
    15121 ttgactttga ccctcttcga ggacagagag atgattgagg aaagactgaa gacctacgca
    15181 cacctttttg atgacaaggt catgaaacaa ctcaagcgcc ggcgctatac tggctggggc
    15241 cggctttctc gcaagctcat caatgggatt cgggataagc aatcaggcaa gacaattttg
    15301 gacttcctca aatccgacgg attcgcaaat aggaatttta tgcagctgat acatgacgac
    15361 tctttgacat tcaaagaaga catacagaag gctcaggtct ccggccaagg agattctttg
    15421 cacgagcata tcgctaactt ggcaggtagc cccgccataa aaaagggcat tcttcaaacg
    15481 gtaaaagttg ttgacgaact cgtgaaggtt atgggccgtc ataagccgga aaacattgtt
    15541 attgaaatgg ctagggaaaa tcagacgacc cagaagggac agaaaaatag cagggagcgg
    15601 atgaagagaa ttgaagaggg aattaaggag cttggatctc agattcttaa ggagcaccct
    15661 gtggagaaca cccaacttca gaatgaaaag ctctaccttt actaccttca aaacggccgg
    15721 gatatgtacg tcgatcagga acttgacatt aaccggttga gcgattatga cgttgacgct
    15781 attgtgcccc aatctttcct taaagacgac tctatcgaca ataaagtgct gacgcgcagc
    15841 gataaaaatc gcggtaagtc ggataatgtc ccgtcggaag aggtggttaa aaaaatgaag
    15901 aactattgga ggcaactcct gaatgccaag ctgatcactc agaggaaatt cgacaatctc
    15961 accaaggcag aaaggggtgg acttagcgag ctcgacaagg ccggttttat caaaagacag
    16021 ctggtggaga cacgccaaat caccaaacac gttgcccaga tcctggattc gaggatgaac
    16081 acgaagtatg acgagaacga caagttgatt agggaagtca aggtcatcac tttgaagtcc
    16141 aagctggtga gcgactttcg caaagacttc cagttttaca aagtcaggga aattaataac
    16201 taccaccacg cccacgacgc ctaccttaac gccgtggttg gcacagcact catcaagaaa
    16261 taccctaagc tcgaatctga gttcgtctat ggcgactata aggtctacga cgttagaaaa
    16321 atgatcgcga aatctgagca ggaaataggc aaggcaactg ccaagtactt cttctattcc
    16381 aatatcatga acttttttaa gacggagatt accctggcga atggtgagat ccgcaagcgc
    16441 cctttgattg agacaaacgg agaaacagga gagatcgtat gggacaaagg gcgggacttt
    16501 gctactgtta ggaaggtgct ctctatgcca caagttaaca ttgtcaaaaa aactgaagtg
    16561 cagacaggtg ggtttagcaa ggaatctatc ctgccgaaga ggaactctga caagctgatc
    16621 gcccgcaaga aagattggga tccgaaaaag tacggaggat tcgactcccc cacagttgcg
    16681 tactccgtgc ttgtcgtggc caaagtggag aagggcaagt ctaagaagct caagagcgtc
    16741 aaagagttgt tggggatcac gattatggag cggtcgtctt tcgaaaagaa tccgatagat
    16801 tttctcgagg ccaagggtta taaagaagtc aagaaggatc ttatcatcaa gctccctaag
    16861 tactccctct ttgagcttga aaacggacgg aaaagaatgc tggcttcagc gggtgaactt
    16921 cagaagggta atgaactcgc tctgccctca aaatatgtga atttccttta cctggcatca
    16981 cactatgaga agcttaaggg gtctccagag gacaacgagc agaagcaact gttcgttgaa
    17041 caacacaagc actaccttga cgagattatc gagcaaatca gcgagtttag caagcgcgtt
    17101 atactggcag acgcaaatct tgataaggtc cttagcgcct acaacaagca tagagacaaa
    17161 cccatccggg agcaggccga gaacattatt catctcttca ccttgacgaa tcttggggcc
    17221 ccggccgcgt tcaagtactt cgatactacc atagacagaa agcgctatac atcgacaaag
    17281 gaagttcttg acgccacgct gatccaccaa agtataacag gcctctatga gacacgcatc
    17341 gacctttcgc agttgggcgg tgacctctct ggcggcagta gtggcggatc ctcaggaagt
    17401 gagactcccg gaacatccga atctgctact ccagagagct caggtggcag ctccggaggt
    17461 agctccacac ttaacattga agacgaatat cgcctccatg agacaagtaa ggaacccgac
    17521 gtttcgcttg gctctacctg gctctccgac ttcccccaag cttgggccga aaccggcggt
    17581 atggggcttg ctgttcgcca ggccccattg ataataccgc tgaaagcgac aagcacacca
    17641 gtttcgatca aacaataccc aatgtctcag gaggcgaggc tcggcattaa gccgcacatc
    17701 caacggttgc tcgaccaggg tattcttgtg ccctgccaat cgccgtggaa cacccccttg
    17761 ttgcccgtta agaaaccagg aaccaacgac tacaggccgg tgcaagactt gcgcgaggtg
    17821 aacaaacggg tggaggatat tcacccgact gtccccaacc catataattt gctttcaggt
    17881 ttgcccccta gccatcagtg gtataccgtg ctggacttga aagatgcctt cttttgtttg
    17941 cgcctccacc cgacttcaca gcccctgttt gcgttcgaat ggcgcgatcc ggagatgggt
    18001 atttcggggc aacttacgtg gacaagactg ccacagggat ttaaaaattc gccaacactc
    18061 ttcaacgagg cccttcacag ggatcttgcc gacttccgca tccagcaccc tgaccttatc
    18121 cttctccagt acgtcgacga tttgcttctc gcagctacta gcgaactcga ctgccagcaa
    18181 ggcacacggg ctctgctcca gacactgggg aaccttggct accgggcctc tgccaaaaaa
    18241 gcgcaaatat gccagaagca ggttaagtac ttggggtatt tgctgaagga ggggcaaagg
    18301 tggctcacag aggcacgcaa agaaacggtc atgggccagc ctaccccaaa aacaccaaga
    18361 caactgcgcg agtttttggg taaagcagga ttttgccggc tgtttatccc gggtttcgcc
    18421 gaaatggccg cacctttgta tccgttgact aagcccggta ctctgtttaa ctggggaccc
    18481 gaccaacaaa aagcatatca agagatcaag caggcattgc tgaccgcgcc ggccctgggt
    18541 ttgcccgact tgaccaaacc gtttgaattg tttgttgacg aaaagcaggg atacgcaaag
    18601 ggcgttctca cgcaaaaact cggaccttgg agaagacccg ttgcatacct tagtaaaaag
    18661 ctggatccgg tggccgctgg atggccaccg tgtttgagga tggtcgccgc tattgcggtc
    18721 cttacgaagg atgccggcaa actgacgatg gggcaaccac ttgttattct tgcacctcac
    18781 gccgtcgagg ctcttgttaa acaaccaccc gatcgctggc tgtctaatgc tcggatgacc
    18841 cattaccaag ctcttttgct ggatacagac cgggttcaat tcgggccggt cgtcgccctc
    18901 aacccggcca ctttgctgcc gctcccagag gaaggcctgc aacacaattg tcttgacatt
    18961 cttgccgagg cccacggtac aaggcccgac ctcaccgacc agcctcttcc ggacgctgac
    19021 catacctggt acacagacgg gagcagtctt ctgcaagagg gacagaggaa agcaggggcc
    19081 gcagttacca ctgaaactga agtgatatgg gctaaagcat tgcccgcggg aactagcgca
    19141 cagcgcgcgg agctcattgc tttgactcag gcactgaaaa tggccgaagg caagaagctg
    19201 aatgtctaca ccgactcgag atatgctttc gctactgccc atatacatgg ggagatatat
    19261 aggcgcaggg gatggctcac ctctgaaggt aaggagatta agaacaaaga tgagatcctg
    19321 gctttgctca aagctttgtt cctccctaag aggttgtcaa taatccattg tcccgggcat
    19381 caaaaaggtc attcggcgga ggctaggggt aacaggatgg cggaccaggc agctcgcaag
    19441 gccgccatta cagagacacc cgatacctca actttgttga tcgaaaactc cagccccagc
    19501 ggaggatcaa aaagaaccgc agacggttcg gaattcgaac ccaaaaagaa gaggaaagtt
    19561 tgaactagtg agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
    19621 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
    19681 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
    19741 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
    19801 taaattatcg cgcg
//
