LOCUS       sequence-351160-        1257 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression plasmid for Saccharomyces cerevisiae 
            beta′-COPI-WD40 (residues 1-301)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Dey D, Singh S, Khan S, Martin M, Schnicker NJ, Gakhar L, Pierce BG,
            Hasan SS
  TITLE     An extended motif in the SARS-CoV-2 spike modulates binding and 
            release of host coatomer in retrograde trafficking.
  JOURNAL   Commun Biol. 2022 Feb 8;5(1):115. doi: 10.1038/s42003-022-03063-y.
  PUBMED    35136165
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-02-8"; volume: "5(1):115. doi"; pages: " 
            10.1038/s42003-022-03063-y"
COMMENT     Sequence Label: pSUMO-beta′-COPI-WD40
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             58..348
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 atgtggtcac acccccaatt tgaaaaaagt gctggtcatc accatcatca tcacgggtcg
       61 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
      121 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
      181 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
      241 ttaagattct tgtacgacgg tattagaatt caagctgatc agacccctga agatttggac
      301 atggaggata acgatattat tgaggctcac cgcgaacaga ttggaggttc gatgaagttg
      361 gatataaaga aaacttttag taatagatcc gatcgagtta aaggtatcga tttccatcct
      421 actgagccat gggttttgac taccttgtac tctggtcgtg ttgagatctg gaattacgaa
      481 acacaagttg aagttagatc catccaagtt acggaaactc ctgttagagc aggtaaattc
      541 attgcaagaa agaattggat aattgttggg agtgatgatt tcaggatcag agtcttcaac
      601 tacaatacag gtgaaaaagt tgttgatttc gaagctcatc cagactatat ccgttccatt
      661 gctgtacatc caacaaagcc ttatgtttta tccggcagtg atgatttgac agtcaaacta
      721 tggaattggg aaaataactg ggccttagaa caaacttttg aaggtcacga acactttgtc
      781 atgtgcgtcg cattcaatcc aaaggaccct agtacgtttg catctgggtg tttggaccga
      841 accgttaaag tttggtcatt gggacaatcg acaccaaatt tcacgttaac cacaggacaa
      901 gaaagaggtg tgaattatgt tgattattac ccgctacctg acaagcctta tatgattact
      961 gcgtcggacg acttaaccat caaaatttgg gattaccaaa cgaagtcatg tgtagccact
     1021 ctagaaggtc acatgtccaa tgtttctttt gctgttttcc atccaacttt acccattata
     1081 atttctggtt ccgaagatgg cactttaaag atatggaatt cttccactta caaggtagaa
     1141 aagactttaa acgttggttt agaaagaagt tggtgtatcg ctacccatcc aacaggacgt
     1201 aaaaactaca ttgcatccgg gtttgataat gggtttaccg tactttcctt gggatga
//
