LOCUS       sequence-351583-        2961 bp    DNA     circular SYN 15-APR-2025
DEFINITION  The plasmid is part of a GG toolkit for cell-cell communication in 
            S. cerevisiae. This plasmid is an L0 part plasmid and contains the 
            Ste2 gene of Saccharomyces cerevisiae (receiver part)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Krink N, Loechner AC, Anders A, Kahnt J, Rensing S, Hochberg G, 
            Sourjik V
  TITLE     Construction of multicellular yeast networks using the communication
            toolkit with variable specificity and attenuation
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pYCTK027
FEATURES             Location/Qualifiers
     source          1..2961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(24..58)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(129..788)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     703..722
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      970..1558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1459..1478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgaggatcc tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
       61 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      121 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      181 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      241 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
      301 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
      361 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      421 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
      481 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
      541 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
      601 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
      661 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
      721 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
      781 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
      841 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
      901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1621 ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcata tgtctgatgc
     1681 ggctccttca ttgagcaatc tattttatga tccaacgtat aatcctggtc aaagcaccat
     1741 taactacact tccatatatg ggaatggatc taccatcact ttcgatgagt tgcaaggttt
     1801 agttaacagt actgttactc aggccattat gtttggtgtc agatgtggtg cagctgcttt
     1861 gactttgatt gtcatgtgga tgacatcgag aagcagaaaa acgccgattt tcattatcaa
     1921 ccaagtttca ttgtttttaa tcattttgca ttctgcactc tattttaaat atttactgtc
     1981 taattactct tcagtgactt acgctctcac cggatttcct cagttcatca gtagaggtga
     2041 cgttcatgtt tatggtgcta caaatataat tcaagtcctt cttgtggctt ctattgagac
     2101 ttcactggtg tttcagataa aagttatttt cacaggcgac aacttcaaaa ggataggttt
     2161 gatgctgacg tcgatatctt tcactttagg gattgctaca gttaccatgt attttgtaag
     2221 cgctgttaaa ggtatgattg tgacttataa tgatgttagt gccacccaag ataaatactt
     2281 caatgcatcc acaattttac ttgcatcctc aataaacttt atgtcatttg tcctggtagt
     2341 taaattgatt ttagctatta gatcaagaag attccttggt cttaagcagt tcgatagttt
     2401 ccatatttta ctcataatgt catgtcaatc tttgttggtt ccatcgataa tattcatcct
     2461 cgcatacagt ttgaaaccaa accagggaac agatgtcttg actactgttg caacattact
     2521 tgctgtattg tctttaccat tatcatcaat gtgggccacg gctgctaata atgcatccaa
     2581 aacaaacaca attacttcag actttacaac atccacagat aggttttatc caggcacgct
     2641 gtctagcttt caaactgata gtatcaacaa cgatgctaaa agcagtctca gaagtagatt
     2701 atatgaccta tatcctagaa ggaaggaaac aacatcggat aaacattcgg aaagaacttt
     2761 tgtttctgag actgcagatg atatagagaa aaatcagttt tatcagttgc ccacacctac
     2821 gagttcaaaa aatactagga taggaccgtt tgctgatgca agttacaaag agggagaagt
     2881 tgaacccgtc gacatgtaca ctcccgatac ggcagctgat gaggaagcca gaaagttctg
     2941 gactgaagat aataataatt t
//
