LOCUS       sequence-351664-       10117 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway destination vector to insert genes of interest having a 
            C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Evans-Yamamoto D, Rouleau FD, Nanda P, Makanae K, Liu Y, Despres PC,
            Matsuo H, Seki M, Dube AK, Ascencio D, Yachie N, Landry CR
  TITLE     Barcode fusion genetics-protein-fragment complementation assay 
            (BFG-PCA): tools and resources that expand the potential for binary 
            protein interaction discovery.
  JOURNAL   Nucleic Acids Res. 2022 Feb 7. pii: 6523796. doi: 
            10.1093/nar/gkac045.
  PUBMED    35137167
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2022 Feb 7. pii: 6523796. doi: 10.1093/nar/gkac045."
COMMENT     Sequence Label: pDEST-DHFR F[3]-C (LEU2)
FEATURES             Location/Qualifiers
     source          1..10117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        541..645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(646..714,715..1506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(864..883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1677..2265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2166..2185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2419..2436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2553..2574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2589..2594,2595..2612,2613..2619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2627..2643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2632..2654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2651..2667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2651..2667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2688..2706
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    4236..4360
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4534..4636
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             4637..5317
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWS*YHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(4703..4722)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5637..5942
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5821..5840
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5983..6107)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      6605..6792
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6660..6684)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(6944..6963)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6945..6963)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6970..6987)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6970..6986)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6979..7001)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7127..7582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7214..7233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7424..7445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7883..8290
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8291..9385
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9879..9898)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9998..10020
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10058..10076)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatcgcttgc ctgtaactta cacgcgcctc gtatctttta atgatggaat aatttgggaa
       61 tttactctgt gtttatttat ttttatgttt tgtatttgga ttttagaaag taaataaaga
      121 aggtagaaga gttacggaat gaagaaaaaa aaataaacaa aggtttaaaa aatttcaaca
      181 aaaagcgtac tttacatata tatttattag acaagaaaag cagattaaat agatatacat
      241 tcgattaacg ataagtaaaa tgtaaaatca caggattttc gtgtgtggtc ttctacacag
      301 acaagatgaa acaattcggc attaatacct gagagcagga agagcaagat aaaaggtagt
      361 atttgttggc gatcccccta gagtctttta catcttcgga aaacaaaaac tattttttct
      421 ttaatttctt tttttacttt ctatttttaa tttatatatt tatattaaaa aatttaaatt
      481 ataattattt ttatagcacg tgatgaaaag gacccaggtg gcacttttcg gggaaatgtg
      541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      661 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      721 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      781 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      841 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      901 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      961 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1021 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1081 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1141 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1201 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1261 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1321 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1381 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1441 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1501 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1561 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1801 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2341 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2401 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2461 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2641 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
     2701 taaagggaac aaaagctggt accgggcccc ccctcgagat ccgggatcga agaaatgatg
     2761 gtaaatgaaa taggaaatca aggagcatga aggcaaaaga caaatataag ggtcgaacga
     2821 aaaataaagt gaaaagtgtt gatatgatgt atttggcttt gcggcgccga aaaaacgagt
     2881 ttacgcaatt gcacaatcat gctgactctg tggcggaccc gcgctcttgc cggcccggcg
     2941 ataacgctgg gcgtgaggct gtgcccggcg gagttttttg cgcctgcatt ttccaaggtt
     3001 taccctgcgc taaggggcga gattggagaa gcaataagaa tgccggttgg ggttgcgatg
     3061 atgacgacca cgacaactgg tgtcattatt taagttgccg aaagaacctg agtgcatttg
     3121 caacatgagt atactagaag aatgagccaa gacttgcgag acgcgagttt gccggtggtg
     3181 cgaacaatag agcgaccatg accttgaagg tgagacgcgc ataaccgcta gagtactttg
     3241 aagaggaaac agcaataggg ttgctaccag tataaataga caggtacata caacactgga
     3301 aatggttgtc tgtttgagta cgctttcaat tcatttgggt gtgcacttta ttatgttaca
     3361 atatggaagg gaactttaca cttctcctat gcacatatat taatgtaact ataacggtcc
     3421 taaggtagcg aaattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct
     3481 cttttccgat ttttttctaa accgtggaat atttcggata tccttttgtt gtttccgggt
     3541 gtacaatatg gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata
     3601 ccttcgttgg tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac
     3661 cagacaagac ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg
     3721 tacataacga actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca
     3781 ctaccctttt tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct
     3841 ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat
     3901 gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt
     3961 ccagagctga tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca
     4021 cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata
     4081 aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt
     4141 ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa
     4201 gctataccaa gcatacaaag ggtgggtcga atcaaacaag tttgtacaaa aaagctgaac
     4261 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     4321 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcgg gtgatgctgc
     4381 caacttagcg gccgctaagt tggcagcatc acccgacgca ctttgcgccg aataaatacc
     4441 tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa
     4501 gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt
     4561 tcaccataat gaaataagat cactaccggg cgtatttttt gagtcatcga gattttcagg
     4621 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     4681 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     4741 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     4801 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     4861 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     4921 ccatgagcaa actgaaacgt tttcatcgct ctggagttaa taccacgacg atttccggca
     4981 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     5041 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     5101 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     5161 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     5221 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     5281 gcaggcgggg cgtaatctag aggatccggc ttactaaaag ccagataaca gtatgcgtat
     5341 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     5401 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     5461 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     5521 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     5581 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     5641 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     5701 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     5761 tgtcagataa agtcccccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     5821 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     5881 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     5941 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     6001 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     6061 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtagg aggaggaagt
     6121 actagtatga gtaaagtaga catggtttgg atagtcggag gcagttctgt ttaccaggaa
     6181 gccatgaatc aaccaggcca cctcagactc tttgtgacaa ggatcatgca ggaatttgaa
     6241 agtgacacgt ttttcccaga aattgatttg gggaaatata aacttctccc agaataccca
     6301 ggcgtcctct ctgaggtcca ggaggaaaaa ggcatcaagt ataagtttga agtctacgag
     6361 aagaaagact aatttaagta agtaatggca aacagctatt atgggtatta tgggttaggg
     6421 ataacagggt aataagctct aagtaagtaa cggccgccac cgcggtggag ctttggactt
     6481 cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga
     6541 aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa
     6601 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     6661 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     6721 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     6781 cacctctacc ggcatgccga gcaaatgcct gcaaatcgct ccccatttca cccaattgta
     6841 gatatgctaa ctccagcaat gagttgatga atctcggtgt gtattttatg tcctcagagg
     6901 acaacacctg ttgtaatcgt tcttccacac ggatcccaat tcgccctata gtgagtcgta
     6961 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7021 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7081 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     7141 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7201 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7261 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7321 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7381 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7441 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7501 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7561 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7621 tttcacaccg catatcgacc ggtcgaggag aacttctagt atatctacat acctaatatt
     7681 attgccttat taaaaatgga atcccaacaa ttacatcaaa atccacattc tcttcaaaat
     7741 caattgtcct gtacttcctt gttcatgtgt gttcaaaaac gttatattta taggataatt
     7801 atactctatt tctcaacaag taattggttg tttggccgag cggtctaagg cgcctgattc
     7861 aagaaatatc ttgaccgcag ttaactgtgg gaatactcag gtatcgtaag atgcaagagt
     7921 tcgaatctct tagcaaccat tatttttttc ctcaacataa cgagaacaca caggggcgct
     7981 atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac
     8041 gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag cgccggaacc
     8101 ggcttttcat atagaataga gaagcgttca tgactaaatg cttgcatcac aatacttgaa
     8161 gttgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc aatcgtctta
     8221 ctttctaact tttcttacct tttacatttc agcaatatat atatatatat ttcaaggata
     8281 taccattcta atgtctgccc ctaagaagat cgtcgttttg ccaggtgacc acgttggtca
     8341 agaaatcaca gccgaagcca ttaaggttct taaagctatt tctgatgttc gttccaatgt
     8401 caagttcgat ttcgaaaatc atttaattgg tggtgctgct atcgatgcta caggtgttcc
     8461 acttccagat gaggcgctgg aagcctccaa gaaggctgat gccgttttgt taggtgctgt
     8521 gggtggtcct aaatggggta ccggtagtgt tagacctgaa caaggtttac taaaaatccg
     8581 taaagaactt caattgtacg ccaacttaag accatgtaac tttgcatccg actctctttt
     8641 agacttatct ccaatcaagc cacaatttgc taaaggtact gacttcgttg ttgtcagaga
     8701 attagtggga ggtatttact ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg
     8761 ggatagtgaa caatacaccg ttccagaagt gcaaagaatc acaagaatgg ccgctttcat
     8821 ggccctacaa catgagccac cattgcctat ttggtccttg gataaagcta atgttttggc
     8881 ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc aagaacgaat tccctacatt
     8941 gaaggttcaa catcaattga ttgattctgc cgccatgatc ctagttaaga acccaaccca
     9001 cctaaatggt attataatca ccagcaacat gtttggtgat atcatctccg atgaagcctc
     9061 cgttatccca ggttccttgg gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa
     9121 gaacaccgca tttggtttgt acgaaccatg ccacggttct gctccagatt tgccaaagaa
     9181 taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa
     9241 cttgcctgaa gaaggtaagg ccattgaaga tgcagttaaa aaggttttgg atgcaggtat
     9301 cagaactggt gatttaggtg gttccaacag taccaccgaa gtcggtgatg ctgtcgccga
     9361 agaagttaag aaaatccttg cttaaaaaga ttctcttttt ttatgatatt tgtacataaa
     9421 ctttataaat gaaattcata atagaaacga cacgaaatta caaaatggaa tatgttcata
     9481 gggtagacga aactatatac gcaatctaca tacatttatc aagaaggaga aaaaggagga
     9541 tgtaaaggaa tacaggtaag caaattgata ctaatggctc aacgtgataa ggaaaaagaa
     9601 ttgcacttta acattaatat tgacaaggag gagggcacca cacaaaaagt taggtgtaac
     9661 agaaaatcat gaaactatga ttcctaattt atatattgga ggattttctc taaaaaaaaa
     9721 aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt taagtcaata
     9781 ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac tctgtcagaa
     9841 acggccttaa cgacgtagtc gatatggtgc actctcagta caatctgctc tgatgccgca
     9901 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9961 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
    10021 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
    10081 taggttaatg tcatgataat aatggtttct taggacg
//
