LOCUS       sequence-351717-       11598 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of mNeonGreen-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, 
            Atsuta-Tsunoda K, Aoki K, Tsukiji S
  TITLE     A chemogenetic platform for controlling plasma membrane signaling 
            and synthetic signal oscillation.
  JOURNAL   Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 
            10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
  PUBMED    35835118
REFERENCE   2  (bases 1 to 11598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.06.005"; journalName: "Cell Chem Biol"; 
            date: "2022-09-15- 15"; volume: "29"; issue: "9"; pages: 
            "1446-1464.e10"
COMMENT     Sequence Label: pCSIIpuro-mNG-eDHFR(69K6)
FEATURES             Location/Qualifiers
     source          1..11598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(35..55)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        40..369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      220..355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     282..301
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        417..464
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             483..857
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    987..1108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1024..1043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1078..1097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1157..1173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1157..1173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1170..1192)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1181..1197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1205..1211,1212..1229,1230..1235))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1250..1271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1388..1405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1559..2147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1639..1658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2318..3109,3110..3178))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2941..2960
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3179..3283)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3358..3377)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3549..3928
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3930..4128
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4083..4103
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4146..4326
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4373..4498
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4990..5223
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5408..5452
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5726..5843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5899..7061
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6130..7052
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7009..7029
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7083..7092
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7089..7796
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      7344..7353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8421..8970
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8565..8582)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8792..8811
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8973..9572
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8973..8992)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9469..9489
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9607..10195
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9660..9680)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10078..10089)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10714..10894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10920..10937)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10926..11150
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11196..11598,1..26)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11283..11302)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11493..11514
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
       61 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      121 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      181 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
      241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      301 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      361 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
      421 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
      481 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
      541 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
      601 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
      661 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
      721 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
      781 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
      841 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
      901 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
      961 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     1021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1081 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     1141 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1201 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1261 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1321 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1381 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1441 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1501 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1561 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1621 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1681 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1741 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1801 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1861 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1921 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1981 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2041 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2101 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2161 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2221 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2281 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2341 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2401 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2461 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2521 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2581 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2641 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2701 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2761 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2821 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2881 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2941 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3001 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3061 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3121 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3181 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3241 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3301 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     3361 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     3421 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     3481 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     3541 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     3601 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     3661 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3721 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3781 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3841 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3901 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3961 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     4021 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     4081 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
     4141 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4201 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4261 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4321 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     4381 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     4441 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     4501 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     4561 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     4621 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     4681 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     4741 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     4801 agagcaaaac aaaagtaaga ccaccgcaca gcaagggccg ctgatcttca gacctggagg
     4861 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     4921 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     4981 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     5041 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     5101 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     5161 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     5221 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     5281 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     5341 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     5401 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     5461 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     5521 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     5581 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     5641 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     5701 tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     5761 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     5821 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagaaat tcactttgaa
     5881 ttaattcaag cttcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5941 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     6001 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     6061 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     6121 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     6181 ccttgcgtgc cttgaattac ttccacctgg ctccagtacg tgattcttga tcccgagctg
     6241 gagccagggg cgggccttgc gctttaggag ccccttcgcc tcgtgcttga gttgaggcct
     6301 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     6361 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     6421 ggcaagatag tcttgtaaat gcgggccagg atctgcacac tggtatttcg gtttttgggg
     6481 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     6541 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     6601 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     6661 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tccagggggc tcaaaatgga
     6721 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa ggggcctttc
     6781 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     6841 attagttctg gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     6901 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     6961 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     7021 cagtggttca aagttttttt cttccatttc aggtgtcgtg aacacgctac cggtccgaat
     7081 tggccaccat ggtgagcaag ggcgaggagg ataacatggc ctctctccca gcgacacatg
     7141 agttacacat ctttggctcc atcaacggtg tggactttga catggtgggt cagggcaccg
     7201 gcaatccaaa tgatggttat gaggagttaa acctgaagtc caccaagggt gacctccagt
     7261 tctccccctg gattctggtc cctcatatcg ggtatggctt ccatcagtac ctgccctacc
     7321 ctgacgggat gtcgcctttc caggccgcca tggtagatgg ctccggctac caagtccatc
     7381 gcacaatgca gtttgaagat ggtgcctccc ttactgttaa ctaccgctac acctacgagg
     7441 gaagccacat caaaggagag gcccaggtga aggggactgg tttccctgct gacggtcctg
     7501 tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc gaagaagact taccccaacg
     7561 acaaaaccat catcagtacc tttaagtgga gttacaccac tggaaatggc aagcgctacc
     7621 ggagcactgc gcggaccacc tacacctttg ccaagccaat ggcggctaac tatctgaaga
     7681 accagccgat gtacgtgttc cgtaagacgg agctcaagca ctccaagacc gagctcaact
     7741 tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat ggacgagctc tacaagtctg
     7801 ggtccgcagc cagtagcgcc ggaagtatga tcagtctgat tgcggcgtta gcggtagatc
     7861 gcgttatcgg catggaaaac gccatgccgt ggaacctgcc tgccgatctc gcctggttta
     7921 aacgcaacac cttaaataaa cccgtgatta tgggccgcca tacctgggaa tcaatcggtc
     7981 gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgggtacg gacaaaaaaa
     8041 agaaaaagaa agatcgcgta acgtgggtga agtcggtgga tgaagccatc gcggcgtgtg
     8101 gtgacgtacc agaaatcatg gtgattggcg gcggtcgcgt ttatgaacag ttcttgccaa
     8161 aagcgcaaaa actgtatctg acgcatatcg acgcagaagt ggaaggcgac acccatttcc
     8221 cggattacga gccggatgac tgggaatcgg tattcagcga attccacgat gctgatgcgc
     8281 agaactctca cagctattgc tttgagattc tggagcggcg gctgtacaag taaagcggcc
     8341 gcggctctag aggatccgtt aactaactta agctagcaac ggtttccctc tagcgggatc
     8401 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     8461 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     8521 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     8581 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     8641 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     8701 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     8761 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     8821 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     8881 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     8941 cgtggttttc ctttgaaaaa cacgataata ccatgaccga gtacaagccc acggtgcgcc
     9001 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     9061 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     9121 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     9181 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     9241 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     9301 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     9361 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     9421 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     9481 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     9541 cctggtgcat gacccgcaag cccggtgcct gactagagtt aacatcgagg gatcaagctt
     9601 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9661 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9721 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9781 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9841 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9901 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9961 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
    10021 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10081 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10141 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
    10201 taccgtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
    10261 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
    10321 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    10381 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
    10441 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
    10501 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
    10561 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
    10621 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
    10681 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
    10741 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10801 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10861 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
    10921 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
    10981 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
    11041 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
    11101 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
    11161 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
    11221 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    11281 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    11341 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    11401 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    11461 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    11521 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    11581 ttggttaaaa aatgagct
//
