LOCUS       sequence-351730-       10813 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Lmna.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Cheng LC, Zhang X, Abhinav K, Nguyen JA, Baboo S, Martinez-Bartolome
            S, Branon TC, Ting AY, Loose E, Yates JR 3rd, Gerace L
  TITLE     Shared and Distinctive Neighborhoods of Emerin and Lamin B Receptor 
            Revealed by Proximity Labeling and Quantitative Proteomics.
  JOURNAL   J Proteome Res. 2022 Sep 2;21(9):2197-2210. doi: 
            10.1021/acs.jproteome.2c00281. Epub 2022 Aug 16.
  PUBMED    35972904
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jproteome.2c00281"; journalName: "J Proteome Res"; 
            date: "2022-09-2- 2"; volume: "21"; issue: "9"; pages: "2197-2210"
COMMENT     Sequence Label: pLV V5-lamin A
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5607..6156
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5751..5768)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5978..5997
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6159..6758
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6159..6178)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6655..6675
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6782..7370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6835..6855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7253..7264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7577..8210
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8338..8354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8338..8354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8351..8373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8362..8378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8386..8392,8393..8410,8411..8416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8431..8452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8569..8586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8740..9328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8820..8839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9499..10290,10291..10359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10122..10141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10360..10464)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10512..10646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10549..10568)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10603..10622
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat ggagaccccg tcacagcggc
     3481 gcgccacccg cagtggggcg caggccagct ctaccccact gtcgcccact cggatcaccc
     3541 ggctgcagga gaaggaggac ctgcaggagc tcaatgaccg cctggccgtg tacatcgatc
     3601 gcgtgcgttc cctggagacc gagaacgcgg ggctgcgcct tcgcatcact gagtctgaag
     3661 aggtggtcag ccgagaggtg tccggcatca aggcggccta cgaggccgag ctgggggatg
     3721 cccgcaagac ccttgattct gtggccaagg agcgcgcccg cctccagcta gagctgagca
     3781 aagtgcgtga ggagttcaag gagctgaagg ctcgcaacac caagaaggag ggggacttgt
     3841 tggctgcgca ggcccggctc aaggacctcg aggctcttct caactccaag gaagctgccc
     3901 tgagcactgc tctcagtgag aagcgcacat tggagggcga gctccatgac ctgcgggggc
     3961 aggtagccaa gcttgaggcg gccctgggag aggctaagaa gcagcttcag gatgagatgc
     4021 tgaggcgagt ggatgctgag aacaggctac agacgctgaa ggaggagctt gacttccaga
     4081 agaacattta cagcgaggaa ctgcgtgaga ccaagcgccg gcatgagacg cggcttgtgg
     4141 agatcgataa cgggaagcag cgagagtttg agagccggct ggcagatgcc ctgcaggagc
     4201 tgcgggctca gcatgaggac caggtggaac agtataagaa ggagctagaa aagacatact
     4261 ccgccaagct ggataatgcc aggcagtctg ctgagaggaa cagcaacctc gtgggggctg
     4321 cccatgagga actgcagcag tctcgaatcc gcattgacag cctctcggcc cagctcagcc
     4381 agctccaaaa gcagttggca gccaaggagg caaagctgcg tgacctggag gactcgctgg
     4441 cccgtgagcg cgataccagc cggcgcctgc tggctgagaa agagcgagag atggcggaga
     4501 tgcgggcgag gatgcagcag cagctggacg agtaccagga gctgctggac atcaagctgg
     4561 ccctggacat ggagatccat gcctatcgaa agctgctgga gggcgaggag gagaggctgc
     4621 gcctgtcccc cagccctacc tcgcagcgca gccgtggccg cgcctcctcc cactcatccc
     4681 agtctcaggg tggaggcagc gtcaccaaaa agcgcaagct ggagtcttcc gagagccgga
     4741 gcagcttctc gcagcatgct cgcactagcg ggcgtgtggc ggtagaggaa gtcgatgaag
     4801 agggaaagtt cgtgcggctg cgcaacaagt ccaacgagga ccagtccatg ggcaactggc
     4861 agatcaggcg tcagaatggt gacgatcctt tgatgaccta tcgcttccca ccgaagttca
     4921 ccctaaaggc tgggcaggtg gtgacgatct gggcttcagg agctggggcc acccatagcc
     4981 cccctactga cttggtgtgg aaggcgcaga acacctgggg ctgtgggagc agccttcgca
     5041 ccgctctcat caactccact ggagaagaag tggccatgcg caagctggtg cgctcactga
     5101 ccatggttga ggacaatgag gatgacgacg aggatggaga agagctcctc catcaccacc
     5161 gtggttccca ctgcagcggc tcgggggacc ccgctgagta caacctgcgc tcacgcaccg
     5221 tgctgtgcgg gacgtgtggg cagcctgctg acaaggctgc cggtggagcg ggagcccagg
     5281 tgggcggatc catctcctct ggctcttctg cctccagtgt cacagtcact cgaagcttcc
     5341 gcagtgtggg gggcagtggg ggtggcagct tcggggacaa cctagtcacc cgctcctacc
     5401 tcctgggcaa ctccagtccc cggagccaga gctcccagaa ctgcagcatc atgtaataat
     5461 gggccgcgac gatgtttaaa cttcgaaacg cgtgaattcc tcgagggcgg ccgctctaga
     5521 gtcgacgggc cgcggtaaca attgttaact aacttaagct agcaacggtt tccctctagc
     5581 gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     5641 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5701 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5761 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5821 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5881 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5941 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     6001 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     6061 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     6121 cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac aagcccacgg
     6181 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6241 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6301 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6361 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6421 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6481 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6541 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6601 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6661 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6721 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc cgggcggggc gcgtctggaa
     6781 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6841 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6901 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6961 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7021 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7081 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7141 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7201 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7261 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7321 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
     7381 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     7441 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     7501 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7561 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat
     7621 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     7681 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     7741 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     7801 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     7861 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     7921 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     7981 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     8041 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8101 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8161 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8221 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8281 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8341 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8401 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8461 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8521 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8581 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8641 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8701 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8761 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8821 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8881 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8941 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9001 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9061 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9121 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9181 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9241 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9301 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9361 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9421 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9481 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9541 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9601 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9661 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9721 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9781 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9841 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9901 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9961 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10021 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10081 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10141 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10201 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10261 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10321 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10381 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10441 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10501 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10621 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10681 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    10741 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    10801 caaacttagt agt
//
