LOCUS       sequence-351804-       11045 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of mouse Nup88-V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11045)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV Nup88-V5
FEATURES             Location/Qualifiers
     source          1..11045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3402..3411
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3406..3415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5661..5702
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5839..6388
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5983..6000)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6210..6229
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6391..6990
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6391..6410)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6887..6907
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7014..7602
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7067..7087)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7485..7496)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7809..8442
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8570..8586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8570..8586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8583..8605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8594..8610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8618..8624,8625..8642,8643..8648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8663..8684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8801..8818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8972..9560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9052..9071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9731..10522,10523..10591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10354..10373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10592..10696)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10744..10878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10781..10800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10835..10854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca tcggcccgcc cgccgccatg gcggctgccg
     3421 tggggccctt gggcgatggg gagctgtggc agagctggct tcctaaccac gtcgtgttct
     3481 tgcggctccg cgagggcgta agaaaccaga gtccagccga agcggagaag ccagcggctt
     3541 cgacttcacc ctcgtgcccg tctctgccgc cgcatttgcc gacgagaaac ctggtcttcg
     3601 gcctcggagg ggaactgttt ctgtgggacg cagaaggcag cgccttcttg gtggttcgcc
     3661 ttcgaggccc cagcggtggt ggcgtggagc cccctctctc ccagtatcag agattactct
     3721 gcattaatcc acccttgttt gaaatccatc aagtcttgtt gagtccaacc caacatcatg
     3781 tagcacttat cggaagtaaa ggacttatgg cattagaatt acctcagagg tgggggaagg
     3841 actctgaatt tgaaggtgga aaagcaactg tgaattgtag caccatcccg attgctgaga
     3901 gatttttcac cagctctacc tctctgactc tgaagcatgc tgcatggtat ccaagtgaga
     3961 tgctggatcc ccacatagtg ctgttgacat cagacaacgt gataagaatt tattctctcc
     4021 gtgagcccca gacacccact aaggtgattg tactttcaga agcagaagag gaaagtttaa
     4081 tactcaataa aggaagagca tatacagcgt ctctaggaga gactgcagtg gcgtttgact
     4141 tcgggcccct ggtaaccgtc tcaaagaata tatttgaaca gaaagacaga gatgtggtgg
     4201 cgtatccact gtacatcctg tatgagaatg gggagacctt ccttacctac gtgagcctgt
     4261 tacacagccc agggaatatt gggaagctgt tgggcccact gcctatgcat cctgcagctg
     4321 aagataacta cggttacgat gcctgtgcta tactctgttt gccctgtgtt ccaaatatct
     4381 tagtaattgc gactgagtca ggaatgctgt accactgtgt tgtactagag ggagaagaag
     4441 aagatgacca aacgttagaa aagtcctggg atcccagggc tgacttcatc ccttctctgt
     4501 acgtgtttga gtgtgttgag ttagagcttg ccctgaagct ggcatctgga gaggacgatc
     4561 cctttgcctc tgacttttcc tgcccaatta aactgcacag agatcccaag tgtccttcga
     4621 gataccactg cagccatgaa gctggcgtgc acagtgtggg gctgacttgg attcacaaac
     4681 tgcacaaatt tcttggatcg gatgaagaag ataaggatag tttacaagaa ctcactgctg
     4741 agcagaaatg ctttgtggag cacattcttt gtacaaagcc attgccgtgc aggcagccag
     4801 ctccaattcg aggattctgg atcgtcccag acatcctggg gcccacaatg atctgcatca
     4861 ccagtaccta tgaatgtctc atacggcctt tattaagtac agtccaccca gcatctcctc
     4921 ccctgctctg tacccaagaa gatgctgaag ttgcagagtc tccactgcgc attctggctg
     4981 aaactccaga ctcctttgag aagcatatta aaagaatctt gcagcgtagt gctgccaacc
     5041 cagcatttct caaatcatct gaaaaggatt tggctccccc tcccgaggag tgtcttcagc
     5101 ttatcagcag agccacccag gtgttccgag aacagtacat tctcaagcag gacctggcca
     5161 aggaggagat tcagcggagg gtcaaattat tatgtgacca aaagaggaaa caactcgaag
     5221 atctcaatta ctgtcgagag gaaaggaaaa gtctccggga aatggctgag cgcttagctg
     5281 acaaatatga ggaagccaaa gaaaaacaag aagatatcat gaacaggatg aaaaaagtgc
     5341 ttcacagttt tcatgctcag ctcccagttc tctctgacag tgagagagac atgaagaaag
     5401 agttacagct gatacctgat caactgcgac atctaggcaa cgccatcaaa caggttacta
     5461 tgaaaaaaga ttatcaacag cggaagatgg aaaaagtgct gagtcctcag aaacccacca
     5521 ttactctcag tgcctaccag cgaaagtgca ttcagtccat cctgaaagaa gagggtgaac
     5581 acataaggga aatggtgaag caaatcaatg acatccgaaa tcatgtcacc ttcggtgggc
     5641 cgggcggggg tggcggggga ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     5701 cgtgagttta aacttcgaaa cgcgtgaatt cctcgagggc ggccgctcta gagtcgacgg
     5761 gccgcggtaa caattgttaa ctaacttaag ctagcaacgg tttccctcta gcgggatcaa
     5821 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5881 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5941 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     6001 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     6061 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     6121 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     6181 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6241 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6301 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     6361 tggttttcct ttgaaaaaca cgataatacc atgaccgagt acaagcccac ggtgcgcctc
     6421 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6481 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6541 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6601 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6661 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6721 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6781 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6841 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6901 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6961 tggtgcatga cccgcaagcc cggtgcctga cccgggcggg gcgcgtctgg aacaatcaac
     7021 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7081 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7141 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7201 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7261 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7321 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7381 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7441 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7501 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7561 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     7621 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     7681 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     7741 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7801 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     7861 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     7921 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     7981 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     8041 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     8101 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     8161 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     8221 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     8281 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8341 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8401 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8461 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     8521 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8581 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8641 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8701 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10741 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10801 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10861 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    10921 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    10981 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    11041 gtagt
//
