LOCUS       sequence-351807-       10582 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression of  V5-tagged mouse Senp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10582)
  TITLE     Gerace lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV V5-Senp2
FEATURES             Location/Qualifiers
     source          1..10582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3387..3396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3396..3437
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5376..5925
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5520..5537)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5747..5766
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5928..6527
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5928..5947)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6424..6444
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6551..7139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6604..6624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7022..7033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7346..7979
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8107..8123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8107..8123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8120..8142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8131..8147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8155..8161,8162..8179,8180..8185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8200..8221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8338..8355)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8509..9097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8589..8608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9268..10059,10060..10128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9891..9910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10129..10233)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10281..10415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10318..10337)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10372..10391
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgccg ccatgggtaa gcctatccct aaccctctcc
     3421 tcggtctcga ttctacgggg ggcggagggg gtgggcccat gtacagatgg ctggctaagg
     3481 ttctcggcac cattcttcgc ttgtgtgagc ggcccgcgcc cggggcccgc gccctactca
     3541 agaggcggcg ctccagcagc actctgtttt ctactgcagt ggacactgat gagatcccag
     3601 ccaaaaggcc aagattagat tgctttattc accaagtgaa gaacagtctc tacaatgctg
     3661 ccagtttatt tggattcccg ttccagctga ccacaaaacc catggtgtct tctgcttgta
     3721 atggaacacg gaatgtggcc ccttctggag aggtcttttc gaactcttca tcttgtgagc
     3781 tgatgagttc tggatcctgc agcagcatgc tgaaactggg taataaatct cctaatggaa
     3841 taagcgacta tccaaagatc agagtgacag tgacccgcga tcagccacgg agagtcctgc
     3901 cctcctttgg ttttacgctg aaatcggaag gctataatag aaggccaagt ggtcgtcgcc
     3961 atagcaaaag caatccagag agttccttaa catggaagcc tcaggagcaa ggtgtgacag
     4021 agatgatttc tgaagagggt ggcaagggtg tgaggcgtcc ccactgcacc gtggaggagg
     4081 gtgtccaaaa ggatgaaaga gaaaagtatc gaaagttatt ggagcgactt aaagaaggtg
     4141 ctcatggaag cacgttccct cccacggttt cacaccacag ttctcagaga attcagatgg
     4201 acacattaaa gaccaaaggc tgggtggagg agcagaatca tggcgtcaga acaacccatt
     4261 ttgttccaaa acagtataga gttgttgaaa caagaggacc tctctgttca atgagaagtg
     4321 aaaagaggta ttcaaaaggg aaagctgata cagagaaggt ggtgggactc cgatttgaaa
     4381 aggaaggtac aagaggacac caaatggagc ctgatctgtc agaagaagtg tctgcccgac
     4441 ttcgtctagg aagtggaagc aatggcttac tcagaaggaa aatatcagtg cttgagataa
     4501 aggaaaagaa cttcccgagc aaagagaagg acagaagaac ggaagacctc tttgaattta
     4561 cagaggacat ggaaaaggaa atcagtaatg ccctgggcca cggcccacca gatgagattt
     4621 tgagcagcgc tttcaagttg cgaatcaccc gcggagacat ccagacctta aagaactatc
     4681 attggctcaa cgatgaggtc attaattttt atatgaatct tttagtggaa aggagtaaaa
     4741 agcaaggtta tccagcactt catgcattca gtacattctt ctatcctaag ctaaagtctg
     4801 gaggttacca agcagtgaaa agatggacca aaggggtaaa tctcttcgaa caagaacttg
     4861 ttctggtacc tattcatcgg aaggtacatt ggagcctggt ggtgatggac ctaagaaaaa
     4921 agtgtcttaa atatctggat tctatgggac aaaaaggcca caggatctgt gagattcttc
     4981 ttcagtattt acaggatgaa agcaagacca aaagaaatac agatctcaac ctcttagagt
     5041 ggacccacta cagcatgaag ccacatgaga ttcctcagca gttgaatggg agtgactgtg
     5101 gaatgtttac ttgtaaatat gcagattata tttctaggga caaacctatc acattcactc
     5161 agcaccagat gcctctcttc cggaagaaga tggtgtggga gatccttcac cagcagttgc
     5221 tgtgataatg ggccgcgacg atgtttaaac ttcgaaacgc gtgaattcct cgagggcggc
     5281 cgctctagag tcgacgggcc gcggtaacaa ttgttaacta acttaagcta gcaacggttt
     5341 ccctctagcg ggatcaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga
     5401 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     5461 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     5521 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     5581 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     5641 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     5701 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     5761 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     5821 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     5881 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg accgagtaca
     5941 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6001 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6061 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6121 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6181 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6241 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6301 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6361 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6421 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6481 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccc gggcggggcg
     6541 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6601 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6661 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6721 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6781 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6841 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6901 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     6961 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7021 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7081 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7141 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     7201 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     7261 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7321 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     7381 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     7441 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     7501 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     7561 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     7621 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     7681 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     7741 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     7801 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7861 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7921 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7981 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8041 gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta
     8101 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8161 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8221 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8281 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8341 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8401 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8461 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8521 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8581 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8641 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8701 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8761 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8821 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8881 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8941 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9001 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9061 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9121 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9181 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9241 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9301 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9361 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9421 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9481 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9541 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9601 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9661 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9721 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9781 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9841 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9901 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9961 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10021 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10081 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10141 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10201 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10261 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
    10321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10381 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10441 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    10501 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10561 tatgtactac aaacttagta gt
//
