LOCUS       sequence-35193-e         912 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Kim do J, Jang JY, Yoon HJ, Suh SW
  TITLE     Crystal structure of YlqF, a circularly permuted GTPase: 
            implications for its GTPase activation in 50 S ribosomal subunit 
            assembly.
  JOURNAL   Proteins. 2008 Sep . 72(4):1363-70.
  PUBMED    18536017
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2008 Sep . 72(4):1363-70."
COMMENT     Sequence Label: ylqF-pET21a
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             846..868
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    883..907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgct taaacccctc
       61 gagtttgctc ttgttgtaca ggagtaatat cttcagggcg ttcgaaggaa acgcgccctg
      121 cttttccctg agccacttct gtgaaaaaaa gcatcaaggc tctttctata tcggggactc
      181 ctcctttttt gaggagtcct ctttttctcg cgaagtcttc gaagaattcg ctgaacgatg
      241 attctattcc aatgcttcga gcgaatatct caaaagctct ttcgaagatt ctctggtctt
      301 ctattctctc taccggtagg cttcccacaa gaaggagctt cgctgcgagg tcctcactga
      361 agatgttctt gtagagaata ccgggtgtgt ccagaatctt cacaccgttc tccagggaga
      421 accactgaat gccctttgtg atgccgggct gtgctccaac ggagctcgct ctttttccct
      481 tcagcttgtt tatgatcgtt gattttcccg tgtttggaac accaacgatc agaacgcggg
      541 caagcctgtc gaaggagagc tttttcagga ggaccttcct tggttcacct ttgtgagtgg
      601 tgatcactct ttttccctgc tttttgaaga actccaccca cttcttcgtt gttttctcgt
      661 ccgctatgtc cactttgttc agaaggatga tcgtttcttt tctagagaag tccacaccgt
      721 acgctgatgt ggcaaagggg gcccgcgcgt cgcggacctc caccactgtg tttacaagcc
      781 tcagaagatc ctttatttgc cgtttcgctt tctctatatg accggggtac cagctcatat
      841 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      901 caattcccct aa
//
