LOCUS       sequence-351984-        5252 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AAV vector for mCre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: pAAV-syn-mCre
FEATURES             Location/Qualifiers
     source          1..5252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    9..233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   235..375
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   235..364
                     /label=AAV2 ITR
     rep_origin      450..905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(537..556)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     747..768
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(922..941)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1041..1063
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        1187..1291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1292..1360,1361..2152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1510..1529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2323..2911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2812..2831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   2973..3102
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        3136..3583
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3595..3604
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3607..3627
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    join(4665..5252,1)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4718..4738)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5136..5147)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctggatcct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
       61 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      121 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
      181 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcaggaac
      241 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
      301 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
      361 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      421 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
      481 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
      541 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      601 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      661 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
      721 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
      781 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
      841 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
      901 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      961 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     1021 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     1081 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1141 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1201 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1261 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1321 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1381 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1441 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1501 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1561 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1621 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1681 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1741 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1801 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1861 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1921 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1981 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2041 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2101 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2161 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2221 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2281 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2341 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2401 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2461 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2521 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2581 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2641 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2701 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2761 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2821 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2881 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2941 cctggccttt tgctggcctt ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct
     3001 cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag
     3061 cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgtcgacgc tagactgcag
     3121 agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc
     3181 ctacctgacg accgaccccg acccactgga caagcaccca acccccattc cccaaattgc
     3241 gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc
     3301 agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct
     3361 cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca
     3421 ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat
     3481 aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc
     3541 tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt cgccgccacc
     3601 atggtgccca agaagaagag gaaggtgagc aacctgctga ccgtgcacca gaacctgccc
     3661 gccctgcccg tggacgccac cagcgacgag gtgcgcaaga acctgatgga catgttccgc
     3721 gaccgccagg ccttcagcga gcacacctgg aagatgctgc tgagcgtgtg ccgcagctgg
     3781 gccgcctggt gcaagctgaa caaccgcaag tggttccccg ccgagcccga ggacgtgcgc
     3841 gactacctgc tgtacctgca ggcccgcggc ctggccgtga agaccatcca gcagcacctg
     3901 ggccagctga acatgctgca ccgccgcagc ggcctgcccc gccccagcga cagcaacgcc
     3961 gtgagcctgg tgatgcgccg catccgcaag gagaacgtgg acgccggcga gcgcgccaag
     4021 caggccctgg ccttcgagcg caccgacttc gaccaggtgc gcagcctgat ggagaacagc
     4081 gaccgctgcc aggacatccg caacctggcc ttcctgggca tcgcctacaa caccctgctg
     4141 cgcatcgccg agatcgcccg catccgcgtg aaggacatca gccgcaccga cggcggccgc
     4201 atgctgatcc acatcggccg caccaagacc ctggtgagca ccgccggcgt ggagaaggcc
     4261 ctgagcctgg gcgtgaccaa gctggtggag cgctggatca gcgtgagcgg cgtggccgac
     4321 gaccccaaca actacctgtt ctgccgcgtg cgcaagaacg gcgtggccgc ccccagcgcc
     4381 accagccagc tgagcacccg ggccctggag ggcatcttcg aggccaccca ccgcctgatc
     4441 tacggcgcca aggacgacag cggccagcgc tacctggcct ggagcggcca cagcgcccgc
     4501 gtgggcgccg cccgcgacat ggcccgcgcc ggcgtgagca tccccgagat catgcaggcc
     4561 ggcggctgga ccaacgtgaa catcgtgatg aactacatcc gcaacctgga cagcgagacc
     4621 ggcgccatgg tgcgcctgct ggaggacggc gactgataat ccggaatcaa cctctggatt
     4681 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4741 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4801 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4861 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4921 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4981 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5041 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5101 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5161 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5221 cgagtcggat ctccctttgg gccgcctccc cg
//
