LOCUS       sequence-352072-        9896 bp    DNA     circular SYN 14-APR-2025
DEFINITION  doxycycline-inducible expression of Human IRF5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Halitzki E, Zahan T, Arh N, 
            Zimmermannova O, Ferreira AG, Li H, Karlsson S, Scheding S, Pereira 
            CF
  TITLE     Single-cell transcriptional profiling informs efficient 
            reprogramming of human somatic cells to cross-presenting dendritic 
            cells.
  JOURNAL   Sci Immunol. 2022 Mar 4;7(69):eabg5539. doi: 
            10.1126/sciimmunol.abg5539. Epub 2022 Mar 4.
  PUBMED    35245086
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2022-03-4"; volume: "7(69):eabg5539. doi"; pages: 
            " 10.1126/sciimmunol.abg5539. Epub 2022 Mar 4"
COMMENT     Sequence Label: pFUW-TetO-IRF5
FEATURES             Location/Qualifiers
     source          1..9896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(20..38,39..61,62..80,81..103,104..122,123..145,
                     146..164,165..187,188..206,207..229,230..248,249..271,
                     272..290)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        323..361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     358..382
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1994..2582
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2047..2067)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2465..2476)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2585..2601)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2586..2602)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3107..3287
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3313..3330)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3319..3543
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3589..4017
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3676..3695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3886..3907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4026..4046)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4031..4360
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4211..4346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4273..4292
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4408..4455
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4474..4848
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4978..5099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5015..5034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5069..5088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5148..5164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5148..5164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5161..5183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5172..5188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5196..5202,5203..5220,5221..5226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5241..5262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5379..5396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5550..6138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5630..5649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6309..7100,7101..7169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6932..6951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7170..7274)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7349..7368)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7540..7919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7921..8119
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8137..8317
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8364..8489
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8982..9215
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9400..9444
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9742..9859
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
       61 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
      121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
      181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
      241 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
      301 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      421 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc accggttcta gaatgaacca
      481 gtccatccca gtggctccca ccccaccccg ccgcgtgcgg ctgaagccct ggctggtggc
      541 ccaggtgaac agctgccagt acccagggct tcaatgggtc aacggggaaa agaaattatt
      601 ctgcatcccc tggaggcatg ccacaaggca tggtcccagc caggacggag ataacaccat
      661 cttcaaggcc tgggccaagg agacagggaa atacaccgaa ggcgtggatg aagccgatcc
      721 ggccaagtgg aaggccaacc tgcgctgtgc ccttaacaag agccgggact tccgcctcat
      781 ctacgacggg ccccgggaca tgccacctca gccctacaag atctacgagg tctgctccaa
      841 tggccctgct cccacagact cccagccccc tgaggattac tcttttggtg caggagagga
      901 ggaggaagaa gaggaagagc tgcagaggat gttgccaagc ctgagcctca cagaggatgt
      961 caagtggccg cccactctgc agccgcccac tctgcggccg cctactctgc agccgcccac
     1021 tctgcagccg cccgtggtgc tgggtccccc tgctccagac cccagccccc tggctcctcc
     1081 ccctggcaac cctgctggct tcagggagct tctctctgag gtcctggagc ctgggcccct
     1141 gcctgccagc ctgccccctg caggcgaaca gctcctgcca gacctgctga tcagccccca
     1201 catgctgcct ctgaccgacc tggagatcaa gtttcagtac cgggggcggc caccccgggc
     1261 cctcaccatc agcaaccccc atggctgccg gctcttctac agccagctgg aggccaccca
     1321 ggagcaggtg gaactcttcg gccccataag cctggagcaa gtgcgcttcc ccagccctga
     1381 ggacatcccc agtgacaagc agcgcttcta cacgaaccag ctgctggatg tcctggaccg
     1441 cgggctcatc ctccagctac agggccagga cctttatgcc atccgcctgt gtcagtgcaa
     1501 ggtgttctgg agcgggcctt gtgcctcagc ccatgactca tgccccaacc ccatccagcg
     1561 ggaggtcaag accaagcttt tcagcctgga gcattttctc aatgagctca tcctgttcca
     1621 aaagggccag accaacaccc caccaccctt cgagatcttc ttctgctttg gggaagaatg
     1681 gcctgaccgc aaaccccgag agaagaagct cattactgta caggtggtgc ctgtagcagc
     1741 tcgactgctg ctggagatgt tctcagggga gctatcttgg tcagctgata gtatccggct
     1801 acagatctca aacccagacc tcaaagaccg catggtggag caattcaagg agctccatca
     1861 catctggcag tcccagcagc ggttgcagcc tgtggcccag gcccctcctg gagcaggcct
     1921 tggtgttggc caggggccct ggcctatgca cccagctggc atgcaataat tcgaagatat
     1981 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2041 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2101 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2161 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2221 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2281 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2341 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2401 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2461 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2521 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2581 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     2641 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     2701 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     2761 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     2821 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     2881 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     2941 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     3001 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     3061 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     3121 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     3181 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     3241 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     3301 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     3361 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     3421 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     3481 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3541 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     3601 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3661 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3721 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3781 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3841 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3901 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3961 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     4021 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4081 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4141 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4261 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4321 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4381 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     4441 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     4501 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     4561 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     4621 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     4681 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     4741 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     4801 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     4861 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4921 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4981 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5041 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5101 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5161 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5221 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5281 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5341 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5401 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5461 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5521 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5581 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5641 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5701 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5761 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5821 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5881 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5941 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6001 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6061 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6121 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6181 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6241 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6301 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6361 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6421 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6481 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6541 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6601 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6661 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6721 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6781 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6841 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6901 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6961 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7021 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7081 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7141 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7201 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7261 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     7321 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7381 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     7441 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     7501 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     7561 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     7621 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7681 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     7741 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     7801 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7861 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7921 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     7981 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     8041 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     8101 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     8161 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8221 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8281 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     8341 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8401 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8461 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8521 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8581 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8641 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     8701 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8761 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8821 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     8881 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     8941 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9001 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9061 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     9121 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     9181 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     9241 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     9301 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     9361 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     9421 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9481 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9541 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     9601 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     9661 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     9721 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     9781 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     9841 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggt
//
