LOCUS       sequence-352089-        6609 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Sars-CoV-2 protein mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: pDON223 Spike D1118H
FEATURES             Location/Qualifiers
     source          1..6609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..28
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(187..214)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(306..392)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     441..463
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     455..472
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     456..472
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    488..587
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4407..4506)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4523..4542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4524..4542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4547..4563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4547..4563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4994..5785
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5878..6466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6367..6386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
       61 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      121 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
      181 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      241 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      301 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      361 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
      421 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
      481 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      541 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatgt tcgtgttcct
      601 ggtgctgctg ccactggtga gctcccagtg cgtgaacctg accaccagaa cccagctgcc
      661 acctgcctac accaactcct tcaccagggg cgtgtactac ccagacaagg tgttcagatc
      721 cagcgtgctg cactctaccc aggacctgtt cctgccattc ttctctaacg tgacctggtt
      781 ccacgccatc cacgtgtctg gaaccaacgg caccaagaga ttcgacaacc cagtgctgcc
      841 attcaacgac ggcgtgtact tcgcttctac cgagaagtct aacatcatca ggggttggat
      901 cttcggaacc accctggact ctaagaccca gtctctgctg atcgtgaaca acgctaccaa
      961 cgtggtgatc aaggtgtgcg agttccagtt ctgcaacgac ccattcctgg gagtgtacta
     1021 ccacaagaac aacaagtctt ggatggaaag cgagttcagg gtgtacagct ctgctaacaa
     1081 ctgcaccttc gagtacgtgt ctcagccttt cctgatggac ctggaaggaa agcagggaaa
     1141 cttcaagaac ctgagagagt tcgtgttcaa gaacatcgac ggttacttca agatatacag
     1201 caagcacacc ccaatcaacc tggtgaggga cctgccacag ggtttctctg ctctggagcc
     1261 cctggtggac ctgccaatcg gcatcaacat caccaggttc cagaccctgc tggctctgca
     1321 caggtcttac ctgaccccag gcgacagcag ctctggatgg accgccggcg ccgctgctta
     1381 ctacgtggga tacctgcagc caaggacctt cctgctgaag tacaacgaaa acggcaccat
     1441 caccgacgcc gtggactgcg ctctggaccc actgtctgag accaagtgca ccctgaagtc
     1501 tttcaccgtg gaaaagggaa tctaccagac cagcaacttc cgcgtgcagc ccaccgagtc
     1561 tatcgtgaga ttcccaaaca tcaccaacct gtgcccattc ggagaagtgt tcaacgctac
     1621 caggttcgcc agcgtgtacg cttggaacag gaagagaatc agcaactgcg tggccgacta
     1681 ctctgtgctg tacaactctg cctccttctc caccttcaag tgctacggcg tgtccccaac
     1741 caagctgaac gacctgtgct tcaccaacgt gtacgctgac tccttcgtga tcagaggaga
     1801 cgaagtgaga cagatcgctc ccggtcagac cggaaagatc gccgactaca actacaagct
     1861 gccagacgac ttcaccggtt gcgtgatcgc ttggaactct aacaacctgg actctaaggt
     1921 gggaggcaac tacaactacc tgtacaggct gttccgcaag agcaacctga agcctttcga
     1981 aagggacatc agcaccgaaa tctaccaggc tggatctacc ccatgcaacg gagtggaagg
     2041 attcaactgc tacttccctc tgcagtctta cggattccag ccaaccaacg gagtgggata
     2101 ccagccatac agagtggtgg tgctgtcttt cgagctgctg cacgctcctg ctaccgtgtg
     2161 cggaccaaag aagtctacca acctggtgaa gaacaagtgc gtgaacttca acttcaacgg
     2221 actgaccgga accggagtgc tgaccgaatc caacaagaag ttcctgcctt tccagcagtt
     2281 cggtagagac atcgccgaca ccaccgacgc tgtgagagac ccacagaccc tggaaatcct
     2341 ggacatcacc ccctgctctt tcggaggagt gagcgtgatc accccaggaa ccaacacctc
     2401 caaccaggtg gccgtgctgt accaggacgt gaactgcacc gaagtgccag tggccatcca
     2461 cgctgaccag ctgaccccaa cctggagagt gtacagcacc ggatctaacg tgttccagac
     2521 ccgtgccggc tgcctgatcg gagccgagca cgtgaacaac tcctacgaat gcgacatccc
     2581 aatcggagct ggcatctgcg cttcttacca gacccagacc aacagcccaa gaagggccag
     2641 atccgtggcc tctcagagca tcatcgccta caccatgagc ctgggcgctg aaaacagcgt
     2701 ggcttacagc aacaactcta tcgccatccc aaccaacttc accatctctg tgaccaccga
     2761 aatcctgcca gtgagcatga ccaagaccag cgtggactgc accatgtaca tctgcggaga
     2821 ctctaccgaa tgctctaacc tgctgctgca gtacggcagc ttctgcaccc agctgaaccg
     2881 cgctctgacc ggaatcgctg tggagcagga caagaacacc caggaagtgt tcgctcaggt
     2941 gaagcagata tacaagaccc cacctatcaa ggacttcgga ggattcaact tctctcagat
     3001 cctgcccgac ccatctaagc cctctaagag gtctttcatc gaagacctgc tgttcaacaa
     3061 ggtgaccctg gccgacgccg gtttcatcaa gcagtacggt gactgcctgg gagacatcgc
     3121 cgctagagac ctgatctgcg cccagaagtt caacggtctg accgtgctgc caccactgct
     3181 gaccgacgag atgatcgccc agtacacctc tgccctgctg gctggaacca tcacctctgg
     3241 ttggaccttc ggagctggcg ccgccctgca gatcccattc gctatgcaga tggcctaccg
     3301 tttcaacggt atcggagtga cccagaacgt gctgtacgaa aaccagaagc tgatcgctaa
     3361 ccagttcaac tctgctatcg gaaagatcca ggactccctg tcctctaccg cttctgccct
     3421 gggaaagctg caggacgtgg tgaaccagaa cgcccaggcc ctgaacaccc tggtgaagca
     3481 gctgtcttct aacttcggag ccatctcctc tgtgctgaac gacatcctgt ctaggctgga
     3541 caaggtggaa gccgaggtgc agatcgacag actgatcacc ggaagactgc agtctctgca
     3601 gacctacgtg acccagcagc tgatcagggc cgctgaaatc agagcttctg ccaacctggc
     3661 tgccaccaag atgtccgaat gcgtgctggg ccagtctaag agggtggact tctgcggaaa
     3721 gggataccac ctgatgtcct tcccacagtc tgctccacac ggagtggtgt tcctgcacgt
     3781 gacctacgtg cccgcccagg aaaagaactt caccaccgcc ccagctatct gccacgacgg
     3841 aaaggctcac ttccccaggg agggagtgtt cgtgtctaac ggaacccact ggttcgtgac
     3901 ccagaggaac ttctacgagc cacagatcat caccacccac aacaccttcg tgtctggaaa
     3961 ctgcgacgtg gtgatcggaa tcgtgaacaa caccgtgtac gacccactgc agccagaact
     4021 ggactctttc aaggaggaac tggacaagta cttcaagaac cacacctctc cagacgtgga
     4081 cctgggagac atctccggaa tcaacgcctc tgtggtgaac atccagaagg aaatcgacag
     4141 actgaacgag gtggccaaga acctgaacga atctctgatc gacctgcagg aactgggaaa
     4201 gtacgagcag tacatcaagt ggccctggta catctggctg ggtttcatcg ctggtctgat
     4261 cgctatcgtg atggtgacca tcatgctgtg ctgcatgacc tcttgctgct cttgcctgaa
     4321 gggatgctgc tcttgcggat cttgctgcaa gttcgacgag gacgactccg agcctgtgct
     4381 gaagggtgtg aagctgcact acacctaccc aactttcttg tacaaagttg gcattataag
     4441 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     4501 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     4561 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     4621 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     4681 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     4741 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     4801 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     4861 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     4921 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     4981 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     5041 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     5101 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     5161 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     5221 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     5281 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     5341 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     5401 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     5461 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     5521 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     5581 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     5641 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     5701 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     5761 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     5821 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6601 cagccgaac
//
