LOCUS       sequence-352515-        4148 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Encodes the X, C and S genes of Hepatitis B Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Crone MA, Freemont PS
  TITLE     Simple Low-Cost Production of DNA MS2 Virus-Like Particles As 
            Molecular Diagnostic Controls.
  JOURNAL   GEN Biotechnol. 2022 Dec 1;1(6):496-503. doi: 
            10.1089/genbio.2022.0033. Epub 2022 Dec 21.
  PUBMED    36644571
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/genbio.2022.0033"; journalName: "GEN Biotechnol"; date: 
            "2022-12-1- 1"; volume: "1"; issue: "6"; pages: "496-503"
COMMENT     Sequence Label: GA_HBV_MS2_(AmpR)
FEATURES             Location/Qualifiers
     source          1..4148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1476..2156
                     /codon_start=1
                     /product="major/small (S) envelope protein of hepatitis B 
                     virus"
                     /label=HBsAg (S)
                     /note="hepatitis B surface antigen"
                     /translation="MENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGS
                     PVCLGQNSQSPTSNHSPTSCPPICPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGML
                     PVCPLIPGSTTTSTGPCKTCTTPAQGNSMFPSCCCTKPTDGNCTCIPIPSSWAFAKYLW
                     EWASVRFSWLSLLVPFVQWFVGLSPTVWLSAIWMMWYWGPSLYSIVSPFIPLLPIFFCL
                     WVYI"
     primer_bind     complement(2291..2308)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2401..2989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2481..2500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3160..3951,3952..4020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3783..3802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4021..4125)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgcatg gtacctcacg tctcaatagg atggctgcta ggctgtactg ccaactggat
      421 ccttcgcggg acgtcctttg tttacgtccc gtcggcgctg aatcccgcgg acgacccctc
      481 tcggggccgc ttgggactct ctcgtcccct tctccgtctg ccgttccagc cgaccacggg
      541 gcgcacctct ctttacgcgg tctccccgtc tgtgccttct catctgccgg tccgtgtgca
      601 cttcgcttca cctctgcacg ttgcatggag accaccgtga acgcccatca gatcctgccc
      661 aaggtcttac ataagaggac tcttggactc ccagcaatgt caacgaccga ccttgaggcc
      721 tacttcaaag actgtgtgtt taaggactgg gaggagctgg gggaggagat taggttaaag
      781 gtctttgtat taggaggctg taggcataaa ttggtctgcg caccagcacc atgcaacttt
      841 ttcacctctg cctaatcatc tcttgtacat gtcccactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttggggcatg gacattgacc cttataaaga atttggagct actgtggagt
      961 tactctcgtt tttgccttct gacttctttc cttccgtcag agatctccta gacaccgcct
     1021 cagctctgta tcgagaagcc ttagagtctc ctgagcattg ctcacctcac catactgcac
     1081 tcaggcaagc cattctctgc tggggggaat tgatgactct agctacctgg gtgggtaata
     1141 atttggaaga tccagcatcc agggatctag tagtcaatta tgttaatact aacatgggtt
     1201 taaagatcag gcaactattg tggtttcata tatcttgcct tacttttgga agagagactg
     1261 tacttgaata tttggtctct ttcggagtgt ggattcgcac tcctccagcc tatagaccac
     1321 caaatgcccc tatcttatca acacttccgg aaactactgt tgttagacga cgggaccgag
     1381 gcaggtcccc tagaagaaga actccctcgc ctcgcagacg cagatctcaa tcgccgcgtc
     1441 gcagaagatc tcaatctcgg gaatctcaat gttagatgga gaacatcaca tcaggattcc
     1501 taggacccct gctcgtgtta caggcggggt ttttcttgtt gacaagaatc ctcacaatac
     1561 cgcagagtct agactcgtgg tggacttctc tcaattttct agggggatca cccgtgtgtc
     1621 ttggccaaaa ttcgcagtcc ccaacctcca atcactcacc aacctcctgt cctccaattt
     1681 gtcctggtta tcgctggatg tgtctgcggc gttttatcat attcctcttc atcctgctgc
     1741 tatgcctcat cttcttattg gttcttctgg attatcaagg tatgttgccc gtttgtcctc
     1801 taattccagg atcaacaaca accagtacgg gaccatgcaa aacctgcacg actcctgctc
     1861 aaggcaactc tatgtttccc tcatgttgct gtacaaaacc tacggatgga aattgcacct
     1921 gtattcccat cccatcgtcc tgggctttcg caaaatacct atgggagtgg gcctcagtcc
     1981 gtttctcttg gctcagttta ctagtgccat ttgttcagtg gttcgtaggg ctttccccca
     2041 ctgtttggct ttcagctata tggatgatgt ggtattgggg gccaagtctg tacagcatcg
     2101 tgagtccctt tataccgctg ttaccaattt tcttttgtct ctgggtatac atttaaacat
     2161 ggagacgagg gagctcctgg gcctcatggg ccttccgctc actgcccgct ttccagtcgg
     2221 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc
     2281 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc
     2341 ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2401 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2461 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2521 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2581 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2641 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2701 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2761 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2821 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2881 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2941 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3001 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3061 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3121 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3181 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3241 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3301 agaaccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3361 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3421 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3481 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3541 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3601 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3661 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3721 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3781 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3841 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3901 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3961 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4021 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4081 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4141 agtgccac
//
