LOCUS       sequence-352591-        6634 bp    DNA     circular SYN 14-APR-2025
DEFINITION  C. elegans mNeonGreen co-injection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Witten G, DeMott E, Huang G, Zelasko F, de Jesus B, Mulchand C, 
            Schuck L, Pullman S, Perez A, Mahableshwarkar P, Wu Z, Cardona EA, 
            Pierce JT, Dickinson DJ, Doonan R
  TITLE     mScarlet and split fluorophore mScarlet resources for plasmid-based 
            CRISPR/Cas9 knock-in in C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 14;2023:10.17912/micropub.biology.000871. 
            doi: 10.17912/micropub.biology.000871. eCollection 2023.
  PUBMED    37396790
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-14"; volume: 
            "2023:10.17912/micropub.biology.000871. doi"; pages: " 
            10.17912/micropub.biology.000871. eCollection 2023"
COMMENT     Sequence Label: pGLOW79
FEATURES             Location/Qualifiers
     source          1..6634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2504..2528
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2837..2846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3396..3420)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4190..4210)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4218..4235)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4218..4234)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4227..4249)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4562..4584
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4622..4640)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4708..4812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4813..4881,4882..5673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSKATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5031..5050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5844..6432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6333..6352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6559..6581
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6578..6594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6578..6594
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agtgattata gtctctgttt tcgttaattt tgaattttgc ttgataaggc tgcaacaaag
       61 atcaggttga catatatttt cagtaattta ttttaacctg tactctatca ctgccggcta
      121 taataagttc ttgaataaaa taattttccc gacaaaacat gagtatttct ttcgaaaata
      181 aaagtgcagg ctaattagag attattctgt aattaactgc ataatttgtc acgtgccata
      241 gttttacatt ccactacgtc atagttctta aaatactaat ctcctgaaaa tagaagtagg
      301 tgaagaaagt ttaattatca gttctaaaat gacaattgat ctttggaata tgttctgaaa
      361 ctaccgatca ttgaacagat gctatttgaa tgatatagaa ttgtatattt gcaatttctg
      421 aaacgcgttc ttaaaggcac acagattaat tcaaaagggt ctggccgcaa aaaggtttat
      481 ggtggccgat tttgagtttt gtgtgtgatt gctttttcac aatcagtgtt ttcaggatta
      541 tgtgatgaac tagatcttca agtttcgtta catttcatat gttttcggaa ctcacgaagt
      601 acatattggg tattgtgctc aaaaaattca gcaatcagct tcgctccgct gactttagaa
      661 cccaaaaaaa tagtatggcc aaactgactg tgttacgatc atttcaattt ttcaatacat
      721 atttaagatt tctaagagta agaaggtcaa aaactgttat ggaatacata tatatttttc
      781 aggttacaat tagtcaaaaa gtgcactgaa atatacgttt taatttcacg aataacccaa
      841 ttagttcaat gtatttttgg tcaaccaacg ttaaagtttg gcttccaacc aattatcatt
      901 tctgatcaac cacaatgttt tttctttatc tgcaagttaa ttttttattt ttatccagat
      961 gtttggcata tttttcaatt cttcactagc gcccacttct tgcacttccg gcgccctgaa
     1021 tctaatgcat ctgttgcaag aattgaaaga ccaatcaaca cattgttttc ttcacgagat
     1081 actgaagaaa atgaataaaa acagagaaaa agagccatgt gattagtgac aactgttgct
     1141 aacagatacc atagcttgga cttggtacgt gatggcaacg tatgggtcaa caaaaatgat
     1201 tgcagagggg gtgcaaaaca gtcaagtcga gaaaatatga aaaacagaaa acaaagaaca
     1261 gaaaaatggg tttgagagtc agtataattt ataaaagaaa aattgtacat agaaattaac
     1321 catttttgta gaagaagtta tttttcaagc atcgttaaaa attattcaaa gcaccttatt
     1381 tcatatttaa ttttaaacat ggttaaatga acaacacggt gcgcaatcag gaaaacttga
     1441 aatctgaaac tgttgttgtg atcttcttcg caactgttca gatagcacta gtgtaatgtt
     1501 aagagtgcgc gaatataatg gaatataatg gatcacacct cctgccatca ggtaaacgtc
     1561 tctgttatca catatttcca actattaaat ttttaccttt tacagtttta catttttttg
     1621 aaaaaagtaa ctttttgtct tcaaaatccc tgacgaaaat atcaaatatt ttaatcgaga
     1681 ctgcagagga accgattgat gatttggaaa atccagcttt acctgtgtaa gaactgaaaa
     1741 gtttcataac cctagggtat tcccagttac attccccact ggctaacaat agcacccagt
     1801 ttttcatcac ctttcttcaa atttctcggc gatttgttaa aaacaaaatt tgtgtccctt
     1861 ctctgatatc tctatgtctc taaacacaag ttcatcggaa aacgaaggag ggtaggtgtt
     1921 ggttgggctc ccgaagtgaa aatagaagag caagaataga atattagaga gagagtgcag
     1981 agagggcggg atagctcccg ggattccgtt ttcttcttct ttatcttcaa cgatgatgtg
     2041 tgtgcgtgtt gtatagattc tgttgctccc ccacaactcg ctccgaaggc tcaatacaat
     2101 tcaattgata ttggagtaga gcctaccgga gtgggaggat aataagaaac ataagaagaa
     2161 gaagaagaag aagcatgctt ctggtttttg atgctatgaa aacggcacaa aaagatgatt
     2221 gaggtccctt ttcaatacct tctctcatct ttcaaatccc attgaaacct aaaacttctc
     2281 accacgcttt accattgttc tccaaaaact tatagcaatg tctataactt ttttatctct
     2341 gaaaagcagt gttccatttt tctttttcct attttatttc aattgtttct cacatttcgt
     2401 ttggattctt tgcttgtcaa ccagcttctt cttccacttt taccgtctaa ttttcagggc
     2461 agggagccat caaacccacg accactagat ccatctagaa atgacaagtt tgtacaaaaa
     2521 agcaggctta atggtctcca agggagagga ggacaacatg gcctccctcc cagccaccca
     2581 cgagctccac atcttcggat ccatcaacgg agtcgacttc gacatggtcg gacaaggaac
     2641 cggaaaccca aacgacggat acgaggagct caacctcaag tccaccaagg taagtttaaa
     2701 catatatata ctaactaacc ctgattattt aaattttcag ggagacctcc aattctcccc
     2761 atggatcctc gtcccacaca tcggatacgg attccaccaa tacctcccat acccagacgg
     2821 aatgtcccca ttccaagccg ccatggtcga cggatccgga taccaagtcc accgtaccat
     2881 gcaattcgag gacggagcct ccctcaccgt caactaccgt tacacctacg agggatccca
     2941 catcaaggta agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggg
     3001 agaggcccaa gtcaagggaa ccggattccc agccgacgga ccagtcatga ccaactccct
     3061 caccgccgcc gactggtgcc gttccaagaa gacctaccca aacgacaagg taagtttaaa
     3121 catgatttta ctaactaact aatctgattt aaattttcag accatcatct ccaccttcaa
     3181 gtggtcctac accaccggaa acggaaagcg ttaccgttcc accgcccgta ccacctacac
     3241 cttcgccaag ccaatggccg ccaactacct caagaaccaa ccaatgtacg tcttccgtaa
     3301 gaccgagctc aagcactcca agaccgagct caacttcaag gagtggcaaa aggccttcac
     3361 cgacgtcatg ggaatggacg agctctacaa gtagtaccca gctttcttgt acaaagtggg
     3421 tgatatctga gctccgcatc ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct
     3481 tacttctaag tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac
     3541 cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt
     3601 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
     3661 gtcgaaaaaa attgtgctcc ctccccccat taataataat tatatcccaa aatctacaca
     3721 atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga aacatcatag
     3781 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
     3841 tatttcttcg cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt
     3901 tggagtattt ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt
     3961 tgctttttgg ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta
     4021 aaatcacaag tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg
     4081 ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt
     4141 tgccttaaat gacagaatac attcacaata taccaaacat aactgtttcc aactttatta
     4201 tacatagttg ataattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4261 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4321 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     4381 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4441 agtgcaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4501 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4561 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4621 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4681 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5041 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5341 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5521 gagcgtgggt ctcgcggtat cattgcagca ctggggtcag atggtaagcc ctcccgtatc
     5581 gtagttatct acacgacggg gagtaaggca actatggatg aacgaaatag acagatcgct
     5641 gagataggtg cctcactgat taagcattgg taactatcag accaagttta ctcatatata
     5701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5821 gtagaaaaga tcaaaggatc ttcttgagat catttttttc tgcgcgtaat ctgctgcttg
     5881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggtctt ttgctggcct
     6481 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6541 tttgagtgag ctgataccag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6601 tacgccaagc tataaacttt gtatagaaaa gttg
//
