LOCUS       sequence-352763-        8775 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HA.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(189..257,258..1049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(407..426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1220..1808
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1709..1728
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1962..1979
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2096..2117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2132..2137,2138..2155,2156..2162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2170..2186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2175..2197
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2194..2210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2194..2210
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2229..2249
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2231..2249
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2277..2503
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2504..2684
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2731..2856
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3349..3582
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3767..3811
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4109..4357
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4109..4129
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4280..4299
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4376..4451
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4499..4616
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4756..5934
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4987..5925
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5882..5902
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5983..5992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6031..6057
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6076..6120
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6130..6837
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6275..6293)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6543..6562
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6907..7495
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6960..6980)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7378..7389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7570..7803
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7875..7996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7912..7931)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7966..7985
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8036..8171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8191..8210)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8192..8210)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8220..8237)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8220..8236)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8229..8251)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8378..8775,1..58)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8465..8484)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8675..8696
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
       61 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      121 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      181 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      241 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      301 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      361 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      421 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      481 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      541 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      601 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      661 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      721 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      781 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      841 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      901 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      961 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1021 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1921 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1981 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2041 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2101 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2161 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2221 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     2281 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     2341 acaaggagag aaaaagcacc gtgcatggcg attggtggaa gtaaggtggt acgatcgtgc
     2401 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     2461 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     2521 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2581 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2641 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     2701 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     2761 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2821 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2881 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2941 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     3001 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3061 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3121 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3181 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3241 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3301 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3361 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     3421 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3481 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3541 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3601 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3661 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3721 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3781 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3841 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3901 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3961 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     4021 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
     4081 ctagtatcga gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt
     4141 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     4201 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     4261 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     4321 cttggcttta tatatcttgt ggaaaggacg aaacaccggg tcttcgagaa gacctgtttt
     4381 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     4441 cgagtcggtg ctttttttaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     4501 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4561 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     4621 tttattacag ggacagcaga gatccacttt ggccgcggct cgaggcgata agctttgcaa
     4681 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     4741 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     4801 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     4861 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     4921 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     4981 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     5041 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
     5101 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
     5161 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
     5221 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
     5281 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     5341 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     5401 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     5461 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     5521 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     5581 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     5641 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     5701 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     5761 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     5821 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     5881 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt
     5941 ctgcagtcga tcgacggtac cgcgggcccg ggatccacgg tcgccaccat gggcgacacc
     6001 tatcggtaca ttggaggagg cgggggaggg tacccatacg atgtgccaga ttacgctgga
     6061 ggaggcgggg gagggaagga gaccgccgcc gccaagttcg agaggcagca catggacagc
     6121 ggcatgctga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     6181 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     6241 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     6301 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     6361 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     6421 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     6481 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     6541 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     6601 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     6661 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     6721 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     6781 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagaaa
     6841 cggccagccg aggatatgga ggaagaacaa gcctttaaac gatctcgata aagcggccgc
     6901 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6961 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7021 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7081 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7141 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7201 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7261 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7321 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7381 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7441 catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggg
     7501 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7561 aggggggact ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta
     7621 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7681 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7741 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7801 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7861 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7921 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7981 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     8041 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8101 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8161 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8221 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8281 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8341 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8401 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8461 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8521 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8581 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8641 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8701 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8761 tattggttaa aaaat
//
