LOCUS       sequence-352790-        9334 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-TRIM33
FEATURES             Location/Qualifiers
     source          1..9334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..38)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     265..285
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(294..722)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(404..425)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     616..635
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    924..972
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     997..1016
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1056..1097)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    1105..1129
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2702..2713
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4589..4613)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4629..5339,5340..5342,5343..5345))
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4671..4692)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5020..5039
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5279..5300
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5449..5473)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5470..5673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5499..5519)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5674..6053
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6225..6244
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6319..6423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6424..6492,6493..7284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6642..6661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7455..8043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7944..7963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8197..8214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8331..8352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8367..8372,8373..8390,8391..8397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8405..8421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8410..8432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8429..8445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8429..8445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8494..8615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8505..8524)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8559..8578
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8773..9171)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(9190..9237)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(9285..9334,1..280))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9299..9334,1..100)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
       61 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      121 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
      181 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
      241 ctggggagcc tggggacttt ccacacccta actgacacac attccacaga attaattcgc
      301 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      361 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      421 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      481 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
      541 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
      601 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
      661 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
      721 gtggggatac cccctagagc cccagctgcg cagatctgct atggcagggc ctgccgcccc
      781 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
      841 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
      901 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc
      961 tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta
     1021 gcctcccccg tttaaactca ttactaaccg gtacgcgtag aatcgagacc gaggagaggg
     1081 ttagggatag gcttcccgtt atcaaccact ttgtacaaga aagctgggtc tagatatctc
     1141 gagagtaact ttatatgtac tggtctctca tctgacttta ggcgttttct gcggggctgt
     1201 ataaagtctt catcagagtc ctcagttacc tcaccatcat cctcttcctg ctcaaactct
     1261 ggcaaaggtg cgaaggtcct gtctgagtag atctctgtga gtttatcttc aaagtacaat
     1321 gcaactgctt tccctgcctg agctacttct gaatcagcct tcaaattaat ctcttgtgtg
     1381 tctgcataaa cttgaacaac tttcatcatt tcattaaacc tttcacagtt cttgaagatc
     1441 aaacggacat cggccacaaa gtcatccggg atttggtagt gttgggaatg ttttttctga
     1501 agcttctttt tcacggtgga taaatccatt ggtttcttta taattttata gtagtttggt
     1561 atcgaagcag gaacaggctc ctggaattca atacttaatt catggcaata gaggtaaagc
     1621 agaagacgtt cacatttcct ttggtccacg gggcttaacc cctgcgcagt tttccccttc
     1681 ttactatgtt gcaaattatc acaatcatat tcaacttctg gctttccaat atctctacaa
     1741 aatgtgcata tccagtcccc acttggaaag ctaagtagtg ttggaacatg acaagttaga
     1801 tgaaagacct ttggacattt ttcgcagcac aagagatctc ctccgttttg gcagacagca
     1861 caccagtctt catttgggtc atcatcttta ttgttgccat ctcctccaat ccttgccgac
     1921 ctgtgcatga ggcttcgaat tggggacttt ccattaacaa ggctgctgag ccctgactgt
     1981 ttgcagcttt cattcatatc tgcaggttca gttttcacat ggttttccag gcttgccaat
     2041 gcatccaatt cactttctag atgcaggttg gttgagagag gtggtgtcaa gctactctca
     2101 ggactgctca acatgcaagc actcctcctg ccatcctctg ttttttcttg ttttacacct
     2161 cctgaaaagc tacatatttc atcttcagtc ccaggttctt gcttgacctt cacctgatca
     2221 gatttgaaac taagacttgt cttctcagca gttcttcctg atgacccaca gctgcctcga
     2281 ctgccagtac tagaagtact tgggggtctc acaggtgtgt gagaattgga taaacctgat
     2341 gatcccggag aaagggcaga gggacctgga gaaggattca tggtgcttgt aggctgtggg
     2401 ggtaggtgac tgcctgagat gtatctactt agtagattat ctaaactact tgagccagca
     2461 tcttccaact gtatgggtgg aatatctggc agcgatggaa ggttttctgg attggtaact
     2521 gaggggatta gctctattgc tgtaacagat gggctggtgg gacctcggtt tgcatttgcc
     2581 atagttgctg tagtagggct cgttgggttg attgtggtgt tgtgtaccga tactacggga
     2641 aagggtccag catgccctgg gtttgagtgt tgtctttgga ggtgtggctg catcatggaa
     2701 tattgagggc cgctgtgcct gggtatccct ggtattctgg cagcattctg agccagtctc
     2761 atctgatggg cttgaaaagc tccacagttc atgttgcctc tttgcattgt ttgcacactg
     2821 atcaatcgag gaggctgttg ttgtaacatc tgaggggctg cttgtctagg atgctgttgt
     2881 gttgttgttg ttgtagttgg tacaggtgct ggtggttgct gcatcctcat ctgttgcaac
     2941 tgctgatgtt tctgtgcata tacttgttgt tgcatgtgct ggagtcgaag ctgtgctaag
     3001 ttaatctgtc caggggtttt actaatgtgg tttgtccctg gaggaacttg cccaactaca
     3061 acattaggag tataaccagg agctggttta ctctctatta ctagattacc taaattgact
     3121 acattctttg cccagaaggt gggatcacaa tggaaacgta ttgctccatt agcagcaggg
     3181 acaggatcac accgtgcttt caaaatatga cgcaactgga aagtaatcag tcgcttgctg
     3241 tatagtagtg ctgtgctgct gccacttgca attgcccaat ttgtgaagtt cataacatgc
     3301 ttcacctgcc gggaaaggcc tgtgatgtca ttctgctgct gtagtaactt catctgtctt
     3361 tcctttgtaa cattctctag ctgttgtaag agagattttc ctttcttatt aatttcattg
     3421 ataagggtga aaatggccac tttaatttcc tgttctactc gtttgttagt ctcatttact
     3481 tcttttatcc tattctgcac ctgagtagct gcaaaatgaa cataattctt cttctcaaga
     3541 agtttcgcca gtagattctc aattgcaccc ttctgatttt gaaaagcttc ttccaaaaac
     3601 tgatacctat gttctttgtg ttccaatagc tgacagtctc tacatgtcaa tctatcacat
     3661 gtttcacaga aaagtttcaa ctgttcttgt ttgtgtacag ggcagaaaac agggcgttga
     3721 ccagatgctc caacagactc tgagacatct tctttcttcc tgatcaagtg atctttagta
     3781 aattttactc tttgatgtgc ttcgatacat gtcttacata gccactctcc acattctaca
     3841 caaaagccaa ctgcacttgc attgtcttca caactagtac atacctgttc tgatttttca
     3901 tcagaactgc taggagcttc agatgtgtct ttcacaaaat aattatccac aaggtctatc
     3961 tgtctgcatt cttggcggca tactgggcac cgtattacac caacttgctg gatgtcgccg
     4021 ttgctgcccc ccgggatggg cacgctgagc tggcgctccg gctcgggcag gcagcgcagg
     4081 cagaaggagt gaagacaggg cagcagcttg ggctccgcct cacgccggct ctgcaagctc
     4141 tgctgacaca cggcgcaggt gtccaggagc gaggctggcg gtccaggagg cggccctgcc
     4201 gagggacccg gagcgggagc cgaggctgga gctggagccg gcgtcgatac tgcgcccccg
     4261 gcaactccag tgcccactga ggccgcagga gatgaagcag cctgggccga gcccgaggag
     4321 gccgcggcca cccccccgtc gtcgggcccg gccgcgccgc cctcagcgcc ggccctgccg
     4381 ccttcctcct cctcctcctc caccagcacc gcggtgagag gcggctccgc ctcctgcgcg
     4441 gcgggcccgg cggccccggc agttaccggc gcgctgccgc tgcccccgcc gccgctctca
     4501 gcctcgccgc cgcctttgtt ttccgccata aggatccagt cgaccatggt taagtatttc
     4561 cttagagaat tggttctatc tccttcgaag cctgcttttt tgtacaaact tgttgatggg
     4621 ctcgagccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     4681 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     4741 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     4801 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc gggtcttgaa gttcaccttg
     4861 atgccgttct tctgcttgtc ggcggtgata tagaccttgt ggctgttgta gttgtactcc
     4921 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     4981 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     5041 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     5101 tgctgcttca tgtggtcggg gtagcgggcg aagcactgca cgccgtaggt gaaggtggtc
     5161 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     5221 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     5281 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     5341 accatggtct cgaggatatc accggtaggg atcctctaga gtccggaggc tggatcggtc
     5401 ccggtgtctt ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt
     5461 cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     5521 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     5581 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     5641 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     5701 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     5761 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     5821 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     5881 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     5941 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     6001 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     6061 atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat
     6121 gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc
     6181 gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat
     6241 tgtactgaga gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc
     6301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6661 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6961 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7561 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8101 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8161 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8221 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8281 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8341 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8401 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8461 ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag tttggacaaa
     8521 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     8581 tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg
     8641 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     8701 ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc ctgctcctcg
     8761 gccacgaagt gcttagccct cccacacata accagagggc agcaattcac gaatcccaac
     8821 tgccgtcggc tgtccatcac tgtccttcac tatggctttg atcccaggat gcagatcgag
     8881 aagcacctgt cggcaccgtc cgcaggggct caagatgccc ctgttctcat ttccgatcgc
     8941 gacgatacaa gtcaggttgc cagctgccgc agcagcagca gtgcccagca ccacgagttc
     9001 tgcacaaggt cccccagtaa aatgatatac attgacacca gtgaagatgc ggccgtcgct
     9061 agagagagct gcgctggcga cgctgtagtc ttcagagatg gggatgctgt tgattgtagc
     9121 cgttgctctt tcaatgaggg tggattcttc ttgagacaaa ggcttggcca tggtttagtt
     9181 cctcaccttg tcgtattata ctatgccgat atactatgcc gatgattaat tgtcaacacg
     9241 tgctgatcag atccgaaaat ggatatacaa gctcccggga gctttttgca aaagcctagg
     9301 cctccaaaaa agcctcctca ctacttctgg aata
//
