LOCUS       sequence-352791-       11857 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Tet-inducible AXL expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Axelrod HD, Valkenburg KC, Amend SR, Hicks JL, Parsana P, Torga G, 
            DeMarzo AM, Pienta KJ
  TITLE     AXL Is a Putative Tumor Suppressor and Dormancy Regulator in 
            Prostate Cancer.
  JOURNAL   Mol Cancer Res. 2019 Feb;17(2):356-369. doi: 
            10.1158/1541-7786.MCR-18-0718. Epub 2018 Oct 5.
  PUBMED    30291220
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1541-7786.MCR-18-0718"; journalName: "Mol Cancer Res"; 
            date: "2019-02"; volume: "17"; issue: "2"; pages: "356-369"
COMMENT     Sequence Label: pLVX-TetOne-Puro-hAXL
FEATURES             Location/Qualifiers
     source          1..11857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..107
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(116..445)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      130..265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(456..1202)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(1221..1731)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1284..1302)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        1748..2115
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    1756..1774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1792..1810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1828..1846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1864..1882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1900..1918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1936..1954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1972..1990
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    4999..5133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5177..5292
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(5555..5599)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5784..6017
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6514..6639
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6686..7319
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7487..7621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7669..7773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7774..7842,7843..8634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7992..8011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8805..9393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9294..9313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9547..9564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9681..9702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9717..9722,9723..9740,9741..9747)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9755..9771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9760..9782
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9779..9795
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9779..9795
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             9923..10556
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10763..11351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             10869..10880
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     11278..11298
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(11365..11857,1..107))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11448..11468)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg
       61 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt cgcgttttgc
      121 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
      181 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
      241 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
      301 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
      361 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
      421 actttccaca ccctaactga cacacattcg cgcgtttacc cggggagcat gtcaaggtca
      481 aaatcgtcaa gagcgtcagc aggcagcata tcaaggtcaa agtcgtcaag ggcatcggct
      541 gggagcatgt ctaagtcaaa atcgtcaagg gcgtcggtcg gcccgccgct ttcgcacttt
      601 agctgtttct ccaggccaca tatgattagt tccaggccga aaaggaaggc aggttcggct
      661 ccctgccggt cgaacagctc aattgcttgt ttcagaagtg ggggcataga atcggtggta
      721 ggtgtctctc tttcctcttt tgctacttga tgctcctgtt cctccaatac gcagcccagt
      781 gtaaagtggc ccacggcgga cagagcgtac agtgcgttct ccagggagaa gccttgctga
      841 cacaggaacg cgagctgatt ttccagggtt tcgtactgtt tctctgttgg gcgggtgccg
      901 agatgcactt tagccccgtc gcgatgtgag aggagagcac agcggtatga cttggcgttg
      961 ttccgcagaa agtcttgcca tgactcgcct tccagggggc aggagtgggt atgatgcctg
     1021 tccagcatct cgattggcag ggcatcgagc agggcccgct tgttcttcac gtgccagtac
     1081 agggtaggct gctcaactcc cagcttttga gcgagtttcc ttgtcgtcag gccttcgata
     1141 ccgactccat tgagtaattc cagagcagag tttatgactt tgctcttgtc cagtctagac
     1201 atggtaattc gatgatcccc ctggggagag aggtcggtga ttcggtcaac gagggagccg
     1261 actgccgacg tgcgctccgg aggcttgcag aatgcggaac accgcgcggg caggaacagg
     1321 gcccacacta ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc gctgctctcg
     1381 gcgcgccctg ctgagcagcc gctattggcc acagcccatc gcggtcggcg cgctgccatt
     1441 gctccctggc gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc cgtttgtcac
     1501 gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc aggaagcgtc
     1561 gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac ggacgtgaag
     1621 aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc agagcagccg
     1681 cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc cgtggaatta
     1741 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
     1801 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
     1861 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
     1921 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
     1981 gtgatagaga acgtataagc tttgcttatg taaaccaggg cgcctataaa agagtgctga
     2041 ttttttgagt aaacttcaat tccacaacac ttttgtctta taccaacttt ccgtaccact
     2101 tcctaccctc gtaaagaatt catggcgtgg cggtgcccca ggatgggcag ggtcccgctg
     2161 gcctggtgct tggcgctgtg cggctgggcg tgcatggccc ccaggggcac gcaggctgaa
     2221 gaaagtccct tcgtgggcaa cccagggaat atcacaggtg cccggggact cacgggcacc
     2281 cttcggtgtc agctccaggt tcagggagag ccccccgagg tacattggct tcgggatgga
     2341 cagatcctgg agctcgcgga cagcacccag acccaggtgc ccctgggtga ggatgaacag
     2401 gatgactgga tagtggtcag ccagctcaga atcacctccc tgcagctttc cgacacggga
     2461 cagtaccagt gtttggtgtt tctgggacat cagaccttcg tgtcccagcc tggctatgtt
     2521 gggctggagg gcttgcctta cttcctggag gagcccgaag acaggactgt ggccgccaac
     2581 acccccttca acctgagctg ccaagctcag ggacccccag agcccgtgga cctactctgg
     2641 ctccaggatg ctgtccccct ggccacggct ccaggtcacg gcccccagcg cagcctgcat
     2701 gttccagggc tgaacaagac atcctctttc tcctgcgaag cccataacgc caagggggtc
     2761 accacatccc gcacagccac catcacagtg ctcccccagc agccccgtaa cctccacctg
     2821 gtctcccgcc aacccacgga gctggaggtg gcttggactc caggcctgag cggcatctac
     2881 cccctgaccc actgcaccct gcaggctgtg ctgtcagacg atgggatggg catccaggcg
     2941 ggagaaccag accccccaga ggagcccctc acctcgcaag catccgtgcc cccccatcag
     3001 cttcggctag gcagcctcca tcctcacacc ccttatcaca tccgcgtggc atgcaccagc
     3061 agccagggcc cctcatcctg gacccactgg cttcctgtgg agacgccgga gggagtgccc
     3121 ctgggccccc ctgagaacat tagtgctacg cggaatggga gccaggcctt cgtgcattgg
     3181 caagagcccc gggcgcccct gcagggtacc ctgttagggt accggctggc gtatcaaggc
     3241 caggacaccc cagaggtgct aatggacata gggctaaggc aagaggtgac cctggagctg
     3301 cagggggacg ggtctgtgtc caatctgaca gtgtgtgtgg cagcctacac tgctgctggg
     3361 gatggaccct ggagcctccc agtacccctg gaggcctggc gcccagggca agcacagcca
     3421 gtccaccagc tggtgaagga accttcaact cctgccttct cgtggccctg gtggtatgta
     3481 ctgctaggag cagtcgtggc cgctgcctgt gtcctcatct tggctctctt ccttgtccac
     3541 cggcgaaaga aggagacccg ttatggagaa gtgtttgaac caacagtgga aagaggtgaa
     3601 ctggtagtca ggtaccgcgt gcgcaagtcc tacagtcgtc ggaccactga agctaccttg
     3661 aacagcttgg gcatcagtga agagctgaag gagaagctgc gggatgtgat ggtggaccgg
     3721 cacaaggtgg ccctggggaa gactctggga gagggagagt ttggagctgt gatggaaggc
     3781 cagctcaacc aggacgactc catcctcaag gtggctgtga agacgatgaa gattgccatc
     3841 tgcacgaggt cagagctgga ggatttcctg agtgaagcgg tctgcatgaa ggaatttgac
     3901 catcccaacg tcatgaggct catcggtgtc tgtttccagg gttctgaacg agagagcttc
     3961 ccagcacctg tggtcatctt acctttcatg aaacatggag acctacacag cttcctcctc
     4021 tattcccggc tcggggacca gccagtgtac ctgcccactc agatgctagt gaagttcatg
     4081 gcagacatcg ccagtggcat ggagtatctg agtaccaaga gattcataca ccgggacctg
     4141 gcggccagga actgcatgct gaatgagaac atgtccgtgt gtgtggcgga cttcgggctc
     4201 tccaagaaga tctacaatgg ggactactac cgccagggac gtatcgccaa gatgccagtc
     4261 aagtggattg ccattgagag tctagctgac cgtgtctaca ccagcaagag cgatgtgtgg
     4321 tccttcgggg tgacaatgtg ggagattgcc acaagaggcc aaaccccata tccgggcgtg
     4381 gagaacagcg agatttatga ctatctgcgc cagggaaatc gcctgaagca gcctgcggac
     4441 tgtctggatg gactgtatgc cttgatgtcg cggtgctggg agctaaatcc ccaggaccgg
     4501 ccaagtttta cagagctgcg ggaagatttg gagaacacac tgaaggcctt gcctcctgcc
     4561 caggagcctg acgaaatcct ctatgtcaac atggatgagg gtggaggtta tcctgaaccc
     4621 cctggagctg caggaggagc tgacccccca acccagccag accctaagga ttcctgtagc
     4681 tgcctcactg cggctgaggt ccatcctgct ggacgctatg tcctctgccc ttccacaacc
     4741 cctagccccg ctcagcctgc tgataggggc tccccagcag ccccagggca ggaggatggt
     4801 gcctgaaccg gtgccggcgg atccagacca cctcccctgc gagctaagct ggacagccaa
     4861 tgacgggtaa gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact
     4921 gcctaatcca aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc
     4981 agcttccgag ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     5041 aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta
     5101 tttgtaacca ttataagctg caataaacaa gttaagtcga taaactggat ctctgctgtc
     5161 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     5221 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     5281 ttttctttta aaggcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt
     5341 ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga
     5401 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
     5461 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
     5521 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
     5581 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
     5641 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct
     5701 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
     5761 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
     5821 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
     5881 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
     5941 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
     6001 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
     6061 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
     6121 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cggccgcttg
     6181 ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     6241 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     6301 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     6361 gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc
     6421 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     6481 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     6541 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     6601 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     6661 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     6721 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     6781 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     6841 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
     6901 tctgagggct cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc
     6961 ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc
     7021 tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc
     7081 cgggtcatcc atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt
     7141 ggcctcttct accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag
     7201 tggatatctg acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt
     7261 gtggtagatc cacagatcaa ggatatcttg tcttctttgg gagtgaatta gcccttccaa
     7321 ctactaagtt tgtagtacat atttaacaaa tacaatttct ttaaaatgaa aataattcag
     7381 aggaatcaca ggtttagagt aaatgaaacc acaggtaatt ggcagtggta atagggtatg
     7441 gggtgggaag tttgggatga ttttggttag cttgagttat ccagttgatc cagacatgat
     7501 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     7561 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     7621 tgatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7981 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8281 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9601 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9661 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9721 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9781 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg taaacgctag
     9841 caatgtcaag gcctctcact ctctgatatt catttctttg caagttataa atactgaata
     9901 ataagatgac atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg
     9961 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
    10021 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
    10081 atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct
    10141 gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt ccccagcgga
    10201 aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat gcagctctcg
    10261 ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt ctctctccgg
    10321 gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc ctttattggc
    10381 ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa aggtcagtgg
    10441 atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg
    10501 gtagatccac agatcaagga tatcttgtct tcgttgggag tgaattagcc cttccagtcc
    10561 cctcttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
    10621 aggtacctga ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc
    10681 acaatcagca ttggtagctg ctgtattgct acttgtgatt gctccatgtt tttctaggtc
    10741 tcgactgcag aattaattcc aggcggggag gcggcccaaa gggagatccg actcgtctga
    10801 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
    10861 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
    10921 gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg
    10981 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
    11041 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
    11101 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
    11161 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
    11221 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
    11281 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
    11341 agaggttgat tgttccagac gcgttcaggc accgggcttg cgggtcatgc accaggtgcg
    11401 cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg
    11461 gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc
    11521 cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc
    11581 gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat
    11641 ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc
    11701 gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc
    11761 gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag
    11821 ctcggtgacc cgctcgatgt ggcggtccgg gtcgacg
//
