LOCUS       sequence-35289-e         851 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3448 (=HL 3425 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             119..130
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    300..316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(368..454)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     607..624
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(710..730)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
       61 gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc atcgtacgtt
      121 tctcctcttt aataagcttt gagcctggct tatcgccggg cttttttatg gcaaaaaaaa
      181 gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc
      241 tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttcc gggagtatct
      301 tgttatccgc tcacaatgtc aatgttatcc gctcacattt atggatcccc taggtctagg
      361 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
      421 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca
      481 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
      541 tcaggtggga ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg
      601 cgttctgatt taatctgtat caggctgaaa atcttctctc atccgccaaa acagccaagc
      661 tagcttttat ttgtagagct catccatgcc atgtgtaatc ccagcagctg ttacaaactc
      721 aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag attgtgtgga
      781 caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata
      841 atggtctgct a
//
