LOCUS       sequence-352984-       10807 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0071
FEATURES             Location/Qualifiers
     source          1..10807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3745..4333
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3798..3818)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4216..4227)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4405..4638
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4716..4837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4753..4772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4807..4826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4877..5012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5032..5051)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5033..5051)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5061..5078)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5061..5077)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5070..5092)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5219..5674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5306..5325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5516..5537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5700..5804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5805..5873,5874..6665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6023..6042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6836..7424
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7325..7344
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7578..7595
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7712..7733
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7748..7753,7754..7771,7772..7778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7786..7802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7791..7813
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7810..7826
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7810..7826
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7845..7865
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7847..7865
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7893..8119
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8120..8300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8347..8472
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8965..9198
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9383..9427
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9658..9868
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9680..9876
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9727..9862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9908..10510
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9908..9927)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10404..10424
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10553..10670
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 gaacgttttt actgtgtcat cagatcctca tccctctcct gcagcccctc cttcactttc
     1261 tttaccctta tcttcatctt ctacctcatc tgggactaaa aaacaaaaga ggaccccaac
     1321 ttatcagaga tctatgagct ttgatccaaa ccttctccac aacaatggac ataatgggta
     1381 ccctaatggt acttcagcag cactgcgtga aactggggtt attgaaaaac tgttaacctc
     1441 ttacggattt attcagtgtt cagaacgtca agctagactt ttcttccact gttcacagta
     1501 taatggcaac ctgcaagact taaaagtagg agatgatgtt gaatttgaag tatcatcgga
     1561 ccgacggact gggaaaccca ttgctgttaa actggtgaag ataaaacaag aaatcctccc
     1621 tgaagaacga atgaatggac aagaagtgtt ttatctgact tacacccctg aagatgtcga
     1681 agggaacgtt cagctggaaa ctggagataa aataaacttt gtaattgata acaataaaca
     1741 tactggtgct gtaagtgctc gcaacattat gctgttgaaa aagaaacaag cccgctgtca
     1801 gggagtagtt tgtgccatga aggaggcatt tggctttatt gaaagaggtg atgttgtaaa
     1861 agagatattc tttcactata gtgaatttaa gggtgactta gaaaccttac agcctggcga
     1921 tgatgtggaa ttcacaatca aggacagaaa tggtaaagaa gttgcaacag atgtcagact
     1981 attgcctcaa ggaacagtca tttttgaaga tatcagcatt gaacattttg aaggaactgt
     2041 aaccaaagtt atcccaaaag tacccagtaa aaaccagaat gacccattgc caggacgcat
     2101 caaagttgac tttgtgatcc ctaaagaact tccctttgga gacaaagata cgaaatccaa
     2161 ggtgaccctg ctggaaggtg accatgttag gtttaatatt tcaacagacc gacgtgacaa
     2221 attagagcga gcaaccaata tagaagttct gtcaaataca tttcagttca ctaatgaagc
     2281 ccgagaaatg ggtgtgattg ctgccatgag agatggtttt ggtttcatca agtgtgtgga
     2341 tcgtgatgtt cgtatgttct tccacttcag tgaaattctg gatgggaacc agctccatat
     2401 tgcagatgaa gtagagttta ctgtggttcc tgatatgctc tctgctcaaa gaaatcatgc
     2461 tattaggatt aaaaaacttc ccaagggcac ggtttcattt cattcccatt cagatcaccg
     2521 ttttctgggc acggtagaaa aagaagccac tttttccaat cctaaaacca ctagcccaaa
     2581 taaaggcaaa gagaaggagg ctgaggatgg cattattgct tatgatgact gtggggtgaa
     2641 actgactatt gcttttcaag ccaaggatgt ggaaggatct acttctcctc aaataggaga
     2701 taaggttgaa tttagtatta gtgacaaaca gaggcctgga cagcaggttg caacttgtgt
     2761 gcgactttta ggtcgtaatt ctaactccaa gaggctcttg ggttatgtgg caactctgaa
     2821 ggataatttt ggatttattg aaacagccaa tcatgataag gaaatctttt tccattacag
     2881 tgagttctct ggtgatgttg atagcctgga actgggggac atggtcgagt atagcttgtc
     2941 caaaggcaaa ggcaacaaag tcagtgcaga aaaagtgaac aaaacacact cagtgaatgg
     3001 cattactgag gaagctgatc ccaccattta ctctggcaaa gtaattcgcc ccctgaggag
     3061 tgttgatcca acacagactg agtaccaagg aatgattgag attgtggagg agggcgatat
     3121 gaaaggtgag gtctatccat ttggcatcgt tgggatggcc aacaaagggg attgcctgca
     3181 gaaaggggag agcgtcaagt tccaattgtg tgtcctgggc caaaatgcac aaactatggc
     3241 ttacaacatc acacccctgc gcagggccac agtggaatgt gtgaaagatc agtttggctt
     3301 cattaactat gaagtaggag atagcaagaa gctctttttc catgtgaaag aagttcagga
     3361 tggcattgag ctacaggcag gagatgaggt ggagttctca gtgattctta atcagcgcac
     3421 tggcaagtgc agcgcctgta atgtttggcg agtctgtgag ggccccaagg ctgttgcagc
     3481 tcctcgacct gatcggttgg tcaatcgctt gaagaatatc actctggatg atgccagtgc
     3541 tcctcgccta atggttcttc gtcagccaag gggaccagat aactcaatgg ggtttggtgc
     3601 agaaagaaag atccgtcaag ctggtgtcat tgactagtaa tgaactagtc cgtaacttga
     3661 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaaata cacaaaccga
     3721 tttattgaca cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3781 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3841 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3901 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3961 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4021 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4081 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4141 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4201 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4261 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4321 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4381 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4441 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4501 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4561 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4621 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     4681 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     4741 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4801 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     4861 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4921 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4981 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     5041 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5101 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5161 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     5221 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5281 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5341 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5401 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5461 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5521 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5581 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5641 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5701 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5761 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5821 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5881 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5941 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6001 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6061 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6121 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6181 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6241 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6301 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6361 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6421 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6481 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6541 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6601 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6661 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6721 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6781 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6841 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6901 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6961 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7021 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7081 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7141 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7201 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7261 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7321 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7381 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7441 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7501 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7561 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7621 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7681 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7741 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7801 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7861 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     7921 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     7981 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     8041 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     8101 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     8161 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8221 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8281 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8341 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     8401 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     8461 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8521 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8581 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8641 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8701 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8761 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8821 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8881 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8941 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9001 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9061 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9121 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9181 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9241 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9301 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9361 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9421 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9481 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9541 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9601 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     9661 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     9721 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9841 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     9901 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     9961 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
    10021 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
    10081 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
    10141 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
    10201 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
    10261 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
    10321 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
    10381 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
    10441 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
    10501 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
    10561 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    10621 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    10681 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
    10741 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
    10801 gggaatt
//
