LOCUS       sequence-352986-       11873 bp    DNA     circular SYN 14-APR-2025
DEFINITION  To convert human skin fibroblasts into induced motor neurons (hiMN) 
            in combination with ISL1, LHX3, FGF2 and two small molecules, 
            forskolin and dorsomorphin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Liu ML, Zang T, Zhang CL
  TITLE     Direct Lineage Reprogramming Reveals Disease-Specific Phenotypes of 
            Motor Neurons from Human ALS Patients.
  JOURNAL   Cell Rep. 2016 Jan 5;14(1):115-28. doi: 
            10.1016/j.celrep.2015.12.018. Epub 2015 Dec 24.
  PUBMED    26725112
REFERENCE   2  (bases 1 to 11873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.12.018"; journalName: "Cell Rep"; date: 
            "2016-01-5- 5"; volume: "14"; issue: "1"; pages: "115-28"
COMMENT     Sequence Label: pCSC-NGN2-IRES-GFP-T2A-Sox11
FEATURES             Location/Qualifiers
     source          1..11873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3957..4524
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3975..4526
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4119..4136)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4346..4365
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4529..4531,4532..4534,4535..5245)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4529..4531,4532..4534,4535..5245)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4574..4595)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4835..4854)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5182..5203
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5264..5317
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      5342..5351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6680..7268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6733..6753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7151..7162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7675..7855
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7881..7898)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7887..8111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8157..8585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8244..8263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8454..8475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8594..8614)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8599..8928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8779..8914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8841..8860
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8976..9023
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9042..9416
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9546..9667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9583..9602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9637..9656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9716..9732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9716..9732)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9729..9751)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9740..9756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9764..9770,9771..9788,9789..9794))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9809..9830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9947..9964)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10118..10706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10198..10217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10877..11668,11669..11737))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11500..11519
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11738..11842)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct agaggatcca
     3121 ccggtgccac catgttcgtc aaatccgaga ccttggagtt gaaggaggaa gaggacgtgt
     3181 tagtgctgct cggatcggcc tcccccgcct tggcggccct gaccccgctg tcatccagcg
     3241 ccgacgaaga agaggaggag gagccgggcg cgtcaggcgg ggcgcgtcgg cagcgcgggg
     3301 ctgaggccgg gcagggggcg cggggcggcg tggctgcggg tgcggagggc tgccggcccg
     3361 cacggctgct gggtctggta cacgattgca aacggcgccc ttcccgggcg cgggccgtct
     3421 cccgaggcgc caagacggcc gagacggtgc agcgcatcaa gaagacccgt agactgaagg
     3481 ccaacaaccg cgagcgaaac cgcatgcaca acctcaacgc ggcactggac gcgctgcgcg
     3541 aggtgctccc cacgttcccc gaggacgcca agctcaccaa gatcgagacc ctgcgcttcg
     3601 cccacaacta catctgggca ctcaccgaga ccctgcgcct ggcggatcac tgcgggggcg
     3661 gcggcggggg cctgccgggg gcgctcttct ccgaggcagt gttgctgagc ccgggaggcg
     3721 ccagcgccgc cctgagcagc agcggagaca gcccctcgcc cgcctccacg tggagttgca
     3781 ccaacagccc cgcgccgtcc tcctccgtgt cctccaattc cacctccccc tacagctgca
     3841 ctttatcgcc cgccagcccg gccgggtcag acatggacta ttggcagccc ccacctcccg
     3901 acaagcaccg ctatgcacct cacctcccca tagccaggga ttgtatctag atgaattccg
     3961 cccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4021 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4081 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4141 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4201 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4261 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4321 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4381 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4441 tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     4501 ttcctttgaa aaacacgatg ataataccat ggtgagcaag ggcgaggagc tgttcaccgg
     4561 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     4621 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     4681 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     4741 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     4801 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     4861 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     4921 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     4981 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5041 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5101 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5161 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5221 tctcggcatg gacgagctgt acaagtccgg actcagatcc tacgagggga gaggaagtct
     5281 tctgacctgc ggagacgtcg aagagaatcc tggacccgct agcgctaccg gactcagatc
     5341 cgccaccatg gtgcagcagg ccgagagctc ggaagccgag agcaacctgc cccgggacgc
     5401 gctggacacc gaggagggcg agttcatggc gtgcagcccg gtggccctgg acgagagcga
     5461 cccggactgg tgcaagacgg cgtcgggcca catcaaacgg cccatgaacg ccttcatggt
     5521 gtggtccaag atcgagcgca ggaagatcat ggagcagtcg cccgacatgc acaacgccga
     5581 gatctccaag aggctgggca agcgctggaa gatgctgaag gacagcgaga agatcccgtt
     5641 catcagggag gcggagcgcc tgcgcctcaa gcacatggct gattatcccg actacaagta
     5701 ccggccgcgc aaaaagccca agacggaccc agcggccaag cccagcgcgg gccagagccc
     5761 cgacaagagc gcggcgggcg ccaaggcagc caagggcccc ggcaagaagt gcgccaagct
     5821 caaggcgcct gcgggcaagg cgggcgcggg caaggcggcg cagccggggg actgcgccgc
     5881 gggcaaggca gccaagtgcg tcttcctgga cgacgacgat gaagacgacg acgaagatga
     5941 cgagctgcag ctacggccca agccggacgc tgacgacgac gacgacgagc ccgcgcactc
     6001 gcacctgctg ccgccgccga cgcagcagca accccctcag ctgctgaggc gctacagcgt
     6061 ggccaaggtc cccgccagcc ccacgctcag cagtgccgcc gagtcccccg agggcgcgag
     6121 cctgtacgac gaagtgcgcg cgggcggccg gctctactac agcttcaaga acatcaccaa
     6181 gcagcagcct ccgcccgcgc ctcccgcgct gtcgcccgcg tcctcccgct gcgtgtccac
     6241 ctcctcatcc agcggcagca gcagcggcag cggcgccgag gatgcagacg acctcatgtt
     6301 cgacctgagc ttgaatttct cccagggcgc gcacagcgcc tgcgagcagc cactgggcgc
     6361 gggagcggcg gggaacctgt ccctgtcgct ggtggataag gacctggatt ccttcagcga
     6421 gggcagcctg ggttcccact tcgagttccc cgactactgc acgccggagc tgagcgagat
     6481 gatcgcgggg gactggctgg aggcgaactt ctccgacctg gtgttcacgt attgaagtac
     6541 gctcgaggga tcaagcttat cgaaacctgg aaaaacatgg agcaatcaca agtagcaata
     6601 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     6661 ttccagtcac acctcagaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6721 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6781 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6841 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6901 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6961 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7021 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7081 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7141 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7201 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7261 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     7321 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7381 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     7441 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     7501 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     7561 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     7621 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     7681 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7741 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7801 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     7861 gctttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     7921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     7981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8101 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     8161 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8221 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8281 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8341 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8401 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8461 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8521 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8581 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     8641 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8701 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     8761 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8821 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     8881 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     8941 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     9001 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     9061 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     9121 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     9181 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     9241 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     9301 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     9361 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     9421 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     9481 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     9541 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9601 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9661 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     9721 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9781 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9841 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9901 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9961 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10021 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10081 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10141 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10561 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10621 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10681 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10741 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10801 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10861 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10921 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10981 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11041 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11101 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11161 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11221 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11281 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11341 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11401 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11461 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11521 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11581 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11641 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11701 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11761 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11821 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
